Objective: To investigate the localization of the novel gene colloctrin in mouse, rat and hu-man kidneys. Methods: The localization of collectrin mRNA and protein was observed by in situ hybridization and immunohistochemical staining, respectively. Results: Collectrin mRNA and protein were mainly localized to collecting duct in rat and human kidneys. It was consistent with the previous finding in mouse. Moreover,collectrin was also observed in distal tubules in rat and human kidneys. Conclusion: Collectrin mainly exists in the collecting duct in mouse, rat and human kidneys.