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学科主题: 临床医学
题名:
Overexpression of heme oxygenase-1 protects smooth muscle cells against oxidative injury and inhibits cell proliferation
作者: Zhang, M1; Zhang, BH1; Chen, L1; An, W1
关键词: heme oxygenase ; retroviral vector ; free radical ; cell proliferation ; vascular smooth muscle cells
刊名: CELL RESEARCH
发表日期: 2002-06-01
卷: 12, 期:2, 页:123-132
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: GENE-TRANSFER ; CARBON-MONOXIDE ; EXPRESSION ; ANTIOXIDANT ; THERAPY ; STRESS ; ATHEROSCLEROSIS ; FIBROBLASTS ; INDUCTION ; REGULATOR
英文摘要:

To investigate whether the expression of exogenous heme oxygenase-1 (HO-1) gene within vascular smooth muscle cells (VSMC) could protect the cells from free radical attack and inhibit cell proliferation, we established an in vitro transfection of human HO-1 gone into rat VSMC mediated by a retroviral vector. The results showed that the profound expression of HO-1 protein as well as HO activity was 1.8- and 2.0-fold increased respectively in the transfected cells compared to the non-transfected ones. The treatment of VSMC with different concentrations of H2O2 led to the remarkable cell damage as indicated by survival rate and LDH leakage. However, the resistance of the HO-1 transfected VSMC against H2O2 was significantly raised. This protective effect was dramatically diminished when the transfected VSMC were pretreated with ZnPP-IX, a specific inhibitor of HO, for 24 h. In addition, we found that the growth potential of the transfected cells was significantly inhibited directly by increased activity of HO-1, and this effect might be related to decreased phosphorylation of MAPK. These results suggest that the overexpression of introduced hHO-1 is potentially able to reduce the risk factors of atherosclerosis, partially due to its cellular protection against oxidative injury and to its inhibitory effect on cellular proliferation.

语种: 英语
WOS记录号: WOS:000176627300005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/49859
Appears in Collections:北京大学第三临床医学院_运动医学研究所_期刊论文

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作者单位: 1.Capital Univ Med Sci, Dept Cell Biol, Beijing 100054, Peoples R China
2.Peking Univ, Inst Sports Med, Hosp 3, Beijing 100083, Peoples R China

Recommended Citation:
Zhang, M,Zhang, BH,Chen, L,et al. Overexpression of heme oxygenase-1 protects smooth muscle cells against oxidative injury and inhibits cell proliferation[J]. CELL RESEARCH,2002,12(2):123-132.
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