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学科主题: 基础医学
题名:
Ginsenoside Rb1 ameliorates lipopolysaccharide-induced albumin leakage from rat mesenteric venules by intervening in both trans-and paracellular pathway
作者: Zhang, Yu1,3,4; Sun, Kai2,3,4; Liu, Yu-Ying2,3,4; Zhang, Yun-Pei1,3,4; Hu, Bai-He2,3,4; Chang, Xin2,3,4; Yan, Li2,3,4; Pan, Chun-Shui2,3,4; Li, Quan2,3,4; Fan, Jing-Yu2,3,4; He, Ke1,3,4; Mao, Xiao-Wei1,3,4; Tu, Lei1,3,4; Wang, Chuan-She1,2,3,4; Han, Jing-Yan1,2,3,4
关键词: hyperpermeability ; caveolin-1 ; nuclear factor-kappa B ; Src ; Panax ginseng
刊名: AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
发表日期: 2014-02-01
DOI: 10.1152/ajpgi.00168.2013
卷: 306, 期:4, 页:G289-G300
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology ; Physiology
研究领域[WOS]: Gastroenterology & Hepatology ; Physiology
关键词[WOS]: INDUCED MICROCIRCULATORY DISTURBANCE ; BLOOD-BRAIN-BARRIER ; ENDOTHELIAL PERMEABILITY ; IN-VIVO ; TYROSINE PHOSPHORYLATION ; VASCULAR-PERMEABILITY ; SIGNALING PATHWAY ; LUNG INJURY ; SRC KINASE ; CAVEOLAE
英文摘要:

This study evaluated whether ginsenoside Rb1 ( Rb1), an ingredient of a Chinese medicine Panax ginseng, has beneficial effects on mesentery microvascular hyperpermeability induced by LPS and the underlying mechanisms. Male Wistar rats were continuously infused with LPS (5 mg (-1) kg center dot h (-1)) via the left jugular vein for 90 min. In some rats, Rb1 (5 mg center dot kg(-1)center dot h (-1)) was administrated through the left jugular vein 30 min after LPS infusion. The dynamics of fluorescein isothiocynatelabeled albumin leakage from mesentery venules was assessed by intravital microscopy. Intestinal tissue edema was evaluated by hematoxylin and eosin staining. The number of caveolae in endothelial cells of microvessels was examined by electron microscopy. Confocal microscopy and Western blotting were applied to detect caveolin-1 (Cav-1) expression and phosphorylation, junction-related proteins, and concerning signaling proteins in intestinal tissues and human umbilical vein endothelial cells. LPS infusion evoked an increased albumin leakage from mesentery venules that was significantly ameliorated by Rb1 posttreatment. Mortality and intestinal edema around microvessels were also reduced by Rb1. Rb1 decreased caveolae number in endothelial cells of microvessels. Cav-1 expression and phosphorylation, VE-Cadherin phosphorylation, ZO-1 degradation, nuclear factor-kappa B (NF-kappa B) activation, and Src kinase phosphorylation were inhibited by Rb1. Rb1 ameliorated microvascular hyperpermeability after the onset of endotoxemia and improved intestinal edema through inhibiting caveolae formation and junction disruption, which was correlated to suppression of NF-kappa B and Src activation.

语种: 英语
所属项目编号: H201-81072909
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000332478600003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/49864
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Peking Univ, Sch Basic Med Sci, Dept Integrat Chinese & Western Med, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Tasly Microcirculat Res Ctr, Beijing 100191, Peoples R China
3.State Adm Tradit Chinese Med China, Key Lab Microcirculat, Beijing, Peoples R China
4.State Adm Tradit Chinese Med, Key Lab Stasis & Phlegm, Beijing, Peoples R China

Recommended Citation:
Zhang, Yu,Sun, Kai,Liu, Yu-Ying,et al. Ginsenoside Rb1 ameliorates lipopolysaccharide-induced albumin leakage from rat mesenteric venules by intervening in both trans-and paracellular pathway[J]. AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY,2014,306(4):G289-G300.
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