IR@PKUHSC  > 北京大学深圳医院
学科主题临床医学
Allergen challenge sensitizes TRPA1 in vagal sensory neurons and afferent C-fiber subtypes in guinea pig esophagus
Liu, Zhenyu1,2; Hu, Youtian2; Yu, Xiaoyun2; Xi, Jiefeng3; Fan, Xiaoming4; Tse, Chung-Ming2; Myers, Allen C.2; Pasricha, Pankaj J.2; Li, Xingde3; Yu, Shaoyong2
关键词Eosinophilic Esophagitis Nodose Jugular Dysphagia Heartburn
刊名AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
2015-03-15
DOI10.1152/ajpgi.00374.2014
308期:6页:G482-G488
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology ; Physiology
资助者NIH ; NIH
研究领域[WOS]Gastroenterology & Hepatology ; Physiology
关键词[WOS]EXPERIMENTAL EOSINOPHILIC ESOPHAGITIS ; MAST-CELL ACTIVATION ; MECHANICAL HYPERSENSITIVITY ; INFLAMMATION ; DERMATITIS ; BRADYKININ ; ANKTM1 ; PAIN
英文摘要

Transient receptor potential A1 (TRPA1) is a newly defined cationic ion channel, which selectively expresses in primary sensory afferent nerve, and is essential in mediating inflammatory nociception. Our previous study demonstrated that TRPA1 plays an important role in tissue mast cell activation-induced increase in the excitability of esophageal vagal nodose C fibers. The present study aims to determine whether prolonged antigen exposure in vivo sensitizes TRPA1 in a guinea pig model of eosinophilic esophagitis (EoE). Antigen challenge-induced responses in esophageal mucosa were first assessed by histological stains and Ussing chamber studies. TRPA1 function in vagal sensory neurons was then studied by calcium imaging and by whole cell patch-clamp recordings in 1,1′-dioctadecyl-3,3,3′, 3′-tetramethylindocarbocyanine perchlorate (DiI)-labeled esophageal vagal nodose and jugular neurons. Extracellular single-unit recordings were performed in vagal nodose and jugular C-fiber neuron subtypes using ex vivo esophageal-vagal preparations with intact nerve endings in the esophagus. Antigen challenge significantly increased infiltrations of eosinophils and mast cells in the esophagus. TRPA1 agonist allyl isothiocyanate (AITC)-induced calcium influx in nodose and jugular neurons was significantly increased, and current densities in esophageal DiI-labeled nodose and jugular neurons were also significantly increased in antigen-challenged animals. Prolonged antigen challenge decreased esophageal epithelial barrier resistance, which allowed intraesophageal-infused AITC-activating nodose and jugular C fibers at their nerve endings. Collectively, these results demonstrated that prolonged antigen challenge sensitized TRPA1 in esophageal sensory neurons and afferent C fibers. This novel finding will help us to better understand the molecular mechanism underlying esophageal sensory and motor dysfunctions in EoE.

语种英语
所属项目编号DK087991
资助者NIH ; NIH
WOS记录号WOS:000351063500003
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/49872
专题北京大学深圳医院
作者单位1.Fudan Univ, Div Gastroenterol & Hepatol, Jinshan Hosp, Shanghai 200433, Peoples R China
2.Peking Univ, Shenzhen Hosp, Div Gastroenterol, Shenzhen, Peoples R China
3.Johns Hopkins Univ, Sch Med, Dept Med, Baltimore, MD 21205 USA
4.Johns Hopkins Univ, Sch Med, Dept Biomed Engn, Baltimore, MD 21205 USA
推荐引用方式
GB/T 7714
Liu, Zhenyu,Hu, Youtian,Yu, Xiaoyun,et al. Allergen challenge sensitizes TRPA1 in vagal sensory neurons and afferent C-fiber subtypes in guinea pig esophagus[J]. AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY,2015,308(6):G482-G488.
APA Liu, Zhenyu.,Hu, Youtian.,Yu, Xiaoyun.,Xi, Jiefeng.,Fan, Xiaoming.,...&Yu, Shaoyong.(2015).Allergen challenge sensitizes TRPA1 in vagal sensory neurons and afferent C-fiber subtypes in guinea pig esophagus.AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY,308(6),G482-G488.
MLA Liu, Zhenyu,et al."Allergen challenge sensitizes TRPA1 in vagal sensory neurons and afferent C-fiber subtypes in guinea pig esophagus".AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY 308.6(2015):G482-G488.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Liu, Zhenyu]的文章
[Hu, Youtian]的文章
[Yu, Xiaoyun]的文章
百度学术
百度学术中相似的文章
[Liu, Zhenyu]的文章
[Hu, Youtian]的文章
[Yu, Xiaoyun]的文章
必应学术
必应学术中相似的文章
[Liu, Zhenyu]的文章
[Hu, Youtian]的文章
[Yu, Xiaoyun]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。