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学科主题: 基础医学
题名:
Isolation and characterization of neural stem cells from human fetal striatum
作者: Li, XX; Xu, JC; Bai, Y; Wang, X; Dai, X; Liu, YN; Zhang, J; Zou, JH; Shen, L; Li, LS
关键词: neural stem cells ; striatum ; adherent ; expansion ; proliferation ; differentiation
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2005-01-14
DOI: 10.1016/j.bbrc.2004.11.044
卷: 326, 期:2, 页:425-434
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: PROGENITOR CELLS ; SPINAL-CORD ; IN-VITRO ; TELOMERASE IMMORTALIZATION ; HUNTINGTONS-DISEASE ; PROLIFERATION ; NEURONS ; BRAIN ; TRANSPLANTATION ; MOUSE
英文摘要:

This paper described that neural stem cells (hsNSCs) were isolated and expanded rapidly from human fetal striatum in adherent culture. The population was serum- and growth factor-dependent and expressed neural stem cell markers. They were capable of multi-differentiation into neurons, astrocytes, and oligodendrocytes. When plated in the dopaminergic neuron inducing medium, human striatum neural stem cells could differentiate into tyrosine hydroxylase positive neurons. hsNSCs were morphologically homogeneous and possessed high proliferation ability. The population doubled every 44.28 h and until now it has divided for more than 82 generations in vitro. Normal human diploid karyotype was unchanged throughout the in vitro culture period. Together, this study has exploited a method for continuous and rapid expansion of human neural stem cells as pure population, which maintained the capacity to generate almost fifty percent neurons. The availability of such cells may hold great interest for basic and applied neuroscience. (C) 2004 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000225997300024
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/49880
Appears in Collections:基础医学院_细胞生物学系_期刊论文

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作者单位: Peking Univ, Hlth Sci Ctr, Dept Cell Biol, Stem Cell Res Ctr, Beijing 100083, Peoples R China

Recommended Citation:
Li, XX,Xu, JC,Bai, Y,et al. Isolation and characterization of neural stem cells from human fetal striatum[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2005,326(2):425-434.
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