IR@PKUHSC  > 北京大学基础医学院  > 细胞生物学系
学科主题基础医学
Isolation and characterization of neural stem cells from human fetal striatum
Li, XX; Xu, JC; Bai, Y; Wang, X; Dai, X; Liu, YN; Zhang, J; Zou, JH; Shen, L; Li, LS
关键词Neural Stem Cells Striatum Adherent Expansion Proliferation Differentiation
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2005-01-14
DOI10.1016/j.bbrc.2004.11.044
326期:2页:425-434
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]PROGENITOR CELLS ; SPINAL-CORD ; IN-VITRO ; TELOMERASE IMMORTALIZATION ; HUNTINGTONS-DISEASE ; PROLIFERATION ; NEURONS ; BRAIN ; TRANSPLANTATION ; MOUSE
英文摘要

This paper described that neural stem cells (hsNSCs) were isolated and expanded rapidly from human fetal striatum in adherent culture. The population was serum- and growth factor-dependent and expressed neural stem cell markers. They were capable of multi-differentiation into neurons, astrocytes, and oligodendrocytes. When plated in the dopaminergic neuron inducing medium, human striatum neural stem cells could differentiate into tyrosine hydroxylase positive neurons. hsNSCs were morphologically homogeneous and possessed high proliferation ability. The population doubled every 44.28 h and until now it has divided for more than 82 generations in vitro. Normal human diploid karyotype was unchanged throughout the in vitro culture period. Together, this study has exploited a method for continuous and rapid expansion of human neural stem cells as pure population, which maintained the capacity to generate almost fifty percent neurons. The availability of such cells may hold great interest for basic and applied neuroscience. (C) 2004 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000225997300024
Citation statistics
Cited Times:24[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/49880
Collection北京大学基础医学院_细胞生物学系
作者单位Peking Univ, Hlth Sci Ctr, Dept Cell Biol, Stem Cell Res Ctr, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Li, XX,Xu, JC,Bai, Y,et al. Isolation and characterization of neural stem cells from human fetal striatum[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2005,326(2):425-434.
APA Li, XX.,Xu, JC.,Bai, Y.,Wang, X.,Dai, X.,...&Li, LS.(2005).Isolation and characterization of neural stem cells from human fetal striatum.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,326(2),425-434.
MLA Li, XX,et al."Isolation and characterization of neural stem cells from human fetal striatum".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 326.2(2005):425-434.
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