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Diversities of podocyte molecular changes induced by different antiproteinuria drugs
Xing, Y; Ding, J; Fan, QF; Guan, N
关键词Nephrin Podocin Cd2ap Alpha-actinin Antiproteinuria Drugs
刊名EXPERIMENTAL BIOLOGY AND MEDICINE
2006-05-01
231期:5页:585-593
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, Research & Experimental
研究领域[WOS]Research & Experimental Medicine
关键词[WOS]PUROMYCIN AMINONUCLEOSIDE NEPHROSIS ; ALPHA-ACTININ ; CD2-ASSOCIATED PROTEIN ; GLOMERULAR PROTEIN ; SLIT-DIAPHRAGM ; NEPHRIN ; EXPRESSION ; RATS ; CYTOSKELETON ; INHIBITION
英文摘要

Nephrin, podocin, MAP, and alpha-actinin-4 are important podocyte proteins that help maintain the integrity of the slit diaphragm and prevent proteinuria. Studies have shown that anglotensin-converting enzyme inhibitors, glucocorticoids, and all-trans retinoic acid (ATRA) have antiproteinuric effects. However, it is still unclear whether these drugs, with different pharmacological mechanisms, lead to a reduction in proteinuria by changing the expression and distribution of these important podocyte proteins. In this study, changes in the expression and distribution of nephrin, podocin, MAP, and alpha-actinin-4 were dynamically detected in Adriamycin-induced nephrotic (ADR) rats treated with three different drugs: lisinopril, prednisone, and ATRA. Nephropathy was induced by an intravenous injection of Adriamycin. After Adriamycin injection, rats received lisinopril, prednisone, and ATRA treatment, respectively. Renal tissues were collected at Days 3, 7, 14, and 28. The distribution and the expression of messenger RNA and protein of nephrin, podocin, CD2AP, and alpha-actinin-4 were detected by indirect immunofluorescence, real-time polymerase chain reaction, and Western blotting, respectively. With the intervention of lisinopril, prednisone, and ATRA, changes in the expression of nephrin, podocin, and CD2AP were diverse, which was different from that detected in ADR rats. After lisinoprif and prednisone intervention, podocin exhibited prominent earlier changes compared with those of nephrin and CD2AP, whereas CD2AP showed more prominent changes after ATRA intervention. There was no change in the expression of a-actinin-4 molecule. In summary, we conclude that the antiproteinuric effects of lisinopril, prednisone, and ATRA were achieved by changes in the expression and distribution of the important podcyte molecules nephrin, podocin, CD2AP, and alpha-actinin-4 The pattern in the change of podocyte molecules after lisinopril and prednisone intervention was similar, but the pattern in the change of podocyte molecules after ATRA intervention was different from that of lisinopril or prednisone intervention.

语种英语
WOS记录号WOS:000237183500013
引用统计
被引频次:21[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/49886
专题北京大学第一临床医学院_儿科
作者单位Peking Univ, Hosp 1, Dept Pediat, Beijing 100034, Peoples R China
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Xing, Y,Ding, J,Fan, QF,et al. Diversities of podocyte molecular changes induced by different antiproteinuria drugs[J]. EXPERIMENTAL BIOLOGY AND MEDICINE,2006,231(5):585-593.
APA Xing, Y,Ding, J,Fan, QF,&Guan, N.(2006).Diversities of podocyte molecular changes induced by different antiproteinuria drugs.EXPERIMENTAL BIOLOGY AND MEDICINE,231(5),585-593.
MLA Xing, Y,et al."Diversities of podocyte molecular changes induced by different antiproteinuria drugs".EXPERIMENTAL BIOLOGY AND MEDICINE 231.5(2006):585-593.
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