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学科主题: 口腔医学
题名:
Enhanced Osteogenic Behavior of ADSCs Produced by Deproteinized Antler Cancellous Bone and Evidence for Involvement of ERK Signaling Pathway
作者: Wei, Jinqi1,2; Xu, Mingming1; Zhang, Xuehui1,3; Meng, Song1; Wang, Yixiang4; Zhou, Tuanfeng2; Ma, Qi1; Han, Bing5; Wei, Yan1; Deng, Xuliang1,6
刊名: TISSUE ENGINEERING PART A
发表日期: 2015-06-01
DOI: 10.1089/ten.tea.2014.0395
卷: 21, 期:11-12, 页:1810-1821
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Cell Biology
研究领域[WOS]: Cell Biology ; Biotechnology & Applied Microbiology
关键词[WOS]: MESENCHYMAL STEM-CELLS ; MARROW STROMAL CELLS ; OSTEOBLAST-LIKE CELLS ; CALCIUM-PHOSPHATE ; REGENERATIVE MEDICINE ; GENE-EXPRESSION ; IONIC PRODUCTS ; DIFFERENTIATION ; ADIPOSE ; HYDROXYAPATITE
英文摘要:

Calcinated antler cancellous bone (CACB) is useful in repair of bone defects, as its composition and architecture is analogous to natural extracellular bone matrix. The use of CACB scaffolds with adipose-derived stem cells (ADSCs) in repair of rabbit mandibular bone defects was investigated along with the underlying molecular signaling pathways involved. CACB promoted the adhesion, spreading, and viability of ADSCs. Increased extracellular matrix production and expression of osteogenic markers in ADSCs were observed when seeded in CACB. The temporal kinetics of mRNA expression of ADSCs cultured in CACB lagged in comparison with that observed in cells grown in medium with osteogenic supplements. Activation of the extracellular signal-related kinases (ERK) 1/2 and RUNX-2 in CACB-cultured ADSCs was observed, and this activation was attenuated by the MeK inhibitor U0126. Microcomputed tomography scanning analysis and histological evaluations showed that loading the CACB with ADSCs resulted in enhanced new bone formation and angiogenesis when the composites were implanted in rabbit mandibular defects. These results indicated that the osteogenic behavior of ADSCs might be driven by the microenvironment formed by CACB via the ERK signaling pathway. These CACB/ADSCs composites have promising therapeutic potential for large bone defect repairs.

语种: 英语
所属项目编号: 2012CB933900 ; 2011DFA32190 ; 51302005 ; 7144256 ; 7144257
项目资助者: National Basic Research Program of China ; Key International S&amp ; T Cooperation Project ; National Natural Science Foundation of China ; Beijing natural science foundation
WOS记录号: WOS:000355091800007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/49888
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Peking Univ Sch & Hosp Stomatol, Dept Geriatr Dent, Beijing, Peoples R China
2.Peking Univ Sch & Hosp Stomatol, Clin Div 1, Beijing, Peoples R China
3.Peking Univ Sch & Hosp Stomatol, Dept Dent Mat, Beijing, Peoples R China
4.Peking Univ Sch & Hosp Stomatol, Cent Lab, Beijing, Peoples R China
5.Peking Univ Sch & Hosp Stomatol, Dept Orthodont, Beijing, Peoples R China
6.Peking Univ Sch & Hosp Stomatol, Natl Engn Lab Digital & Mat Technol Stomatol, Beijing, Peoples R China

Recommended Citation:
Wei, Jinqi,Xu, Mingming,Zhang, Xuehui,et al. Enhanced Osteogenic Behavior of ADSCs Produced by Deproteinized Antler Cancellous Bone and Evidence for Involvement of ERK Signaling Pathway[J]. TISSUE ENGINEERING PART A,2015,21(11-12):1810-1821.
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