IR@PKUHSC  > 北京大学口腔医学院
学科主题口腔医学
Enhanced Osteogenic Behavior of ADSCs Produced by Deproteinized Antler Cancellous Bone and Evidence for Involvement of ERK Signaling Pathway
Wei, Jinqi1,2; Xu, Mingming1; Zhang, Xuehui1,3; Meng, Song1; Wang, Yixiang4; Zhou, Tuanfeng2; Ma, Qi1; Han, Bing5; Wei, Yan1; Deng, Xuliang1,6
刊名TISSUE ENGINEERING PART A
2015-06-01
DOI10.1089/ten.tea.2014.0395
21期:11-12页:1810-1821
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Cell Biology
研究领域[WOS]Cell Biology ; Biotechnology & Applied Microbiology
关键词[WOS]MESENCHYMAL STEM-CELLS ; MARROW STROMAL CELLS ; OSTEOBLAST-LIKE CELLS ; CALCIUM-PHOSPHATE ; REGENERATIVE MEDICINE ; GENE-EXPRESSION ; IONIC PRODUCTS ; DIFFERENTIATION ; ADIPOSE ; HYDROXYAPATITE
英文摘要

Calcinated antler cancellous bone (CACB) is useful in repair of bone defects, as its composition and architecture is analogous to natural extracellular bone matrix. The use of CACB scaffolds with adipose-derived stem cells (ADSCs) in repair of rabbit mandibular bone defects was investigated along with the underlying molecular signaling pathways involved. CACB promoted the adhesion, spreading, and viability of ADSCs. Increased extracellular matrix production and expression of osteogenic markers in ADSCs were observed when seeded in CACB. The temporal kinetics of mRNA expression of ADSCs cultured in CACB lagged in comparison with that observed in cells grown in medium with osteogenic supplements. Activation of the extracellular signal-related kinases (ERK) 1/2 and RUNX-2 in CACB-cultured ADSCs was observed, and this activation was attenuated by the MeK inhibitor U0126. Microcomputed tomography scanning analysis and histological evaluations showed that loading the CACB with ADSCs resulted in enhanced new bone formation and angiogenesis when the composites were implanted in rabbit mandibular defects. These results indicated that the osteogenic behavior of ADSCs might be driven by the microenvironment formed by CACB via the ERK signaling pathway. These CACB/ADSCs composites have promising therapeutic potential for large bone defect repairs.

语种英语
WOS记录号WOS:000355091800007
项目编号2012CB933900 ; 2011DFA32190 ; 51302005 ; 7144256 ; 7144257
资助机构National Basic Research Program of China ; Key International S&amp ; T Cooperation Project ; National Natural Science Foundation of China ; Beijing natural science foundation
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/49888
专题北京大学口腔医学院
北京大学第一临床医学院_超声诊断中心
北京大学口腔医学院_特诊科
北京大学口腔医学院_中心实验室
北京大学临床肿瘤学院_乳腺癌预防治疗中心
作者单位1.Peking Univ Sch & Hosp Stomatol, Dept Geriatr Dent, Beijing, Peoples R China
2.Peking Univ Sch & Hosp Stomatol, Clin Div 1, Beijing, Peoples R China
3.Peking Univ Sch & Hosp Stomatol, Dept Dent Mat, Beijing, Peoples R China
4.Peking Univ Sch & Hosp Stomatol, Cent Lab, Beijing, Peoples R China
5.Peking Univ Sch & Hosp Stomatol, Dept Orthodont, Beijing, Peoples R China
6.Peking Univ Sch & Hosp Stomatol, Natl Engn Lab Digital & Mat Technol Stomatol, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Wei, Jinqi,Xu, Mingming,Zhang, Xuehui,et al. Enhanced Osteogenic Behavior of ADSCs Produced by Deproteinized Antler Cancellous Bone and Evidence for Involvement of ERK Signaling Pathway[J]. TISSUE ENGINEERING PART A,2015,21(11-12):1810-1821.
APA Wei, Jinqi.,Xu, Mingming.,Zhang, Xuehui.,Meng, Song.,Wang, Yixiang.,...&Deng, Xuliang.(2015).Enhanced Osteogenic Behavior of ADSCs Produced by Deproteinized Antler Cancellous Bone and Evidence for Involvement of ERK Signaling Pathway.TISSUE ENGINEERING PART A,21(11-12),1810-1821.
MLA Wei, Jinqi,et al."Enhanced Osteogenic Behavior of ADSCs Produced by Deproteinized Antler Cancellous Bone and Evidence for Involvement of ERK Signaling Pathway".TISSUE ENGINEERING PART A 21.11-12(2015):1810-1821.
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