|Activation of NF-kappa B in bronchial epithelial cells from children with asthma|
|Zhao, SY1; Qi, Y1; Liu, XH1; Jiang, QB1; Liu, SY1; Jiang, Y1; Jiang, ZF1|
|关键词||Nuclear Factor-kappa b Epithelial Cells Asthma|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
Objectives To determine whether nuclear factor-kappaB (NF-kappaB) is activated in epithelial cells from children with asthma and to understand the role of NF-kappaB in airway inflammation in asthma.
Methods Bronchial mucosa specimens were obtained from 9 children with asthma and 6 control subjects. NF-kappaB expression in epithelial cells were detected by Immunohistochemical examination, and NF-kappaB-DNA binding was measured by electrophoretic mobility shift assay (EMSA).
Results Nuclear expression of NF-kappaB in epithelia[ cells was observed in the 9 asthmatic children. NF-kappaB-DNA binding was found in 4 asthmatic children (EMSA was performed in 6 asthmatic children). In contrast, both nuclear expression and NF-kappaB-DNA binding were absent in the 6 control subjects.
Conclusion These results indicated that NF-epsilonB is activated in epithelial cells from asthmatic children and the NF-kappaB activation may be the basis for the increased expression of many inflammatory genes and for airway inflammation in asthma.
|作者单位||1.Beijing Inst Pediat Res, Pulm Funct Lab, Beijing 100045, Peoples R China|
2.Beijing Univ, Clin Hosp 1, Cent Lab, Beijing 100034, Peoples R China
|Zhao, SY,Qi, Y,Liu, XH,et al. Activation of NF-kappa B in bronchial epithelial cells from children with asthma[J]. CHINESE MEDICAL JOURNAL,2001,114(9):909-911.|
|APA||Zhao, SY.,Qi, Y.,Liu, XH.,Jiang, QB.,Liu, SY.,...&Jiang, ZF.(2001).Activation of NF-kappa B in bronchial epithelial cells from children with asthma.CHINESE MEDICAL JOURNAL,114(9),909-911.|
|MLA||Zhao, SY,et al."Activation of NF-kappa B in bronchial epithelial cells from children with asthma".CHINESE MEDICAL JOURNAL 114.9(2001):909-911.|