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学科主题临床医学
Altered methylation of IGF2 DMR0 is associated with neural tube defects
Wu, Lihua1; Wang, Li1; Shangguan, Shaofang1; Chang, Shaoyan1; Wang, Zhen1; Lu, Xiaolin1; Zhang, Qin1; Wang, Jianhua1; Zhao, Huizhi1; Wang, Fang1; Guo, Jin1; Niu, Bo1; Guo, Jingzhu2; Zhang, Ting1
关键词Igf2 Neural Tube Defects Differentially Methylated Region Dna Methylation Epigenetics
刊名MOLECULAR AND CELLULAR BIOCHEMISTRY
2013-08-01
DOI10.1007/s11010-013-1655-1
380期:1-2页:33-42
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]IMPRINTED GENES ; FOLATE-DEFICIENCY ; DNA METHYLATION ; GROWTH-FACTORS ; SEX-RATIOS ; FACTOR-II ; CANCER ; PROLIFERATION ; RECEPTORS ; DISTINCT
英文摘要

Neural tube defects (NTDs) are serious congenital malformation of fusion failure of the neural tube during early embryogenesis. DNA methylation disorders have been found in NTD-affected fetuses, and are correlated to the risk of NTDs. The insulin-like growth factor 2 (IGF2) gene, maternally imprinted, has a key role in fetal development. IGF2 transcription is partly controlled by differentially methylated regions (DMRs) 0 and 2. To assess whether disturbed methylation pattern increases the incidence of NTDs, we employed matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) to quantify CpG methylation levels of DMR2 and 0 in fetuses with or without NTDs. We found that the methylation level of IGF2 DMR0 increased significantly in the brain tissues of NTD-affected fetuses. And hypermethylation of DMR0 was associated with an increased risk of NTDs, with an odds ratio of 5.375 (95 % CI: 1.447-19.965; p = 0.007). IGF2 mRNA expression was negatively correlated with the methylation level of DMR0 (R (2) = 0.893; p = 0.000) in HCT15 cells. These results highlights that IGF2 DMR0 hypermethylation is a potential risk factor of NTD, and IGF2 gene is a promising candidate gene to study for a greater understanding of the cause of NTDs.

语种英语
WOS记录号WOS:000321131100004
项目编号201002006 ; 81000249 ; 81150008
资助机构special research projects of health industry "infant malnutrition assessment and intervention" ; National Natural Science Fund of China ; Young Scientists Research Fund of Beijing Municipal Health Bureau
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/49902
专题北京大学第二临床医学院
北京大学第二临床医学院_医务处
作者单位1.Capital Inst Pediat, Beijing Municipal Key Lab Child Dev & Nutri, Beijing 100020, Peoples R China
2.Peking Univ, Peoples Hosp, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
Wu, Lihua,Wang, Li,Shangguan, Shaofang,et al. Altered methylation of IGF2 DMR0 is associated with neural tube defects[J]. MOLECULAR AND CELLULAR BIOCHEMISTRY,2013,380(1-2):33-42.
APA Wu, Lihua.,Wang, Li.,Shangguan, Shaofang.,Chang, Shaoyan.,Wang, Zhen.,...&Zhang, Ting.(2013).Altered methylation of IGF2 DMR0 is associated with neural tube defects.MOLECULAR AND CELLULAR BIOCHEMISTRY,380(1-2),33-42.
MLA Wu, Lihua,et al."Altered methylation of IGF2 DMR0 is associated with neural tube defects".MOLECULAR AND CELLULAR BIOCHEMISTRY 380.1-2(2013):33-42.
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