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Down-regulation of DJ-1 protein in the ejaculated spermatozoa from Chinese asthenozoospermia patients
Zhang, Zi-Yu1,2; An, Chun-Na1,2; Jiang, Hui3; Wang, Qi2; Yuan, Ren-Pei3; Liu, Jian-Ming3; Shi, Wan-Lu1,2; Pu, Xiao-Ping1,2
关键词Dj-1 Asthenozoospermia Male Infertility Superoxide Dismutase
刊名FERTILITY AND STERILITY
2011-07-01
DOI10.1016/j.fertnstert.2011.04.048
96期:1页:19-U652
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]SPERM PROTEIN ; OXIDATIVE DAMAGE ; EXPRESSION ; SP22 ; IDENTIFICATION ; FERTILIZATION ; LOCALIZATION ; PROFILES ; EXPOSURE ; STRESS
英文摘要

Objective: To determine whether the expression of DJ-1 protein, whose levels in spermatozoa have been reported to be highly correlated with male infertility caused by toxicants, is changed in spermatozoa of Chinese asthenozoospermia patients.

Design: DJ-1 measurement by Western blotting, quantitive ELISA, and isoelectric-focusing electrophoresis (IFE) combined with immunoblotting.

Setting: Academic medical center and research laboratories.

Patient(s): Asthenozoospermia patients (n = 113), including mild asthenozoospermia patients (n = 70) and moderate asthenozoospermia patients (n = 43), and age-matched control subjects (n = 58). Intervention(s): None.

Main Outcome Measure(s): DJ-1 in spermatozoawas determined by Western blotting and ELISA, the isoelectric point (pI) of DJ-1 by IFE combined with immunoblotting, and sperm superoxide dismutase (SOD) activity by an assay kit.

Result(s): The sperm DJ-1 concentration in moderate asthenozoospermia patients was lower than those in mild asthenozoospermia patients and control subjects. DJ-1 with a more acidic pI was increased in asthenozoospermia patients. Sperm SOD activity was decreased in asthenozoospermia patients.

Conclusion(s): DJ-1 levels are reduced in moderate asthenozoospermia patients. DJ-1 concentration is positively correlated with sperm motility and sperm SOD activity indicated by partial correlation analysis. (Fertil Steril (R) 2011;96:19-23. (C) 2011 by American Society for Reproductive Medicine.)

语种英语
WOS记录号WOS:000292283000025
资助机构State Key Laboratory of Natural and Biomimetic Drugs, Peking University, People&prime ; s Republic of China
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/49907
专题北京大学药学院
北京大学药学院_分子与细胞药理学系
作者单位1.Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
2.Peking Univ, Dept Mol & Cellular Pharmacol, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
3.Peking Univ, Hosp 3, Dept Androl, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Zi-Yu,An, Chun-Na,Jiang, Hui,et al. Down-regulation of DJ-1 protein in the ejaculated spermatozoa from Chinese asthenozoospermia patients[J]. FERTILITY AND STERILITY,2011,96(1):19-U652.
APA Zhang, Zi-Yu.,An, Chun-Na.,Jiang, Hui.,Wang, Qi.,Yuan, Ren-Pei.,...&Pu, Xiao-Ping.(2011).Down-regulation of DJ-1 protein in the ejaculated spermatozoa from Chinese asthenozoospermia patients.FERTILITY AND STERILITY,96(1),19-U652.
MLA Zhang, Zi-Yu,et al."Down-regulation of DJ-1 protein in the ejaculated spermatozoa from Chinese asthenozoospermia patients".FERTILITY AND STERILITY 96.1(2011):19-U652.
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