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学科主题: 药学
题名:
Down-regulation of DJ-1 protein in the ejaculated spermatozoa from Chinese asthenozoospermia patients
作者: Zhang, Zi-Yu1,2; An, Chun-Na1,2; Jiang, Hui3; Wang, Qi2; Yuan, Ren-Pei3; Liu, Jian-Ming3; Shi, Wan-Lu1,2; Pu, Xiao-Ping1,2
关键词: DJ-1 ; asthenozoospermia ; male infertility ; superoxide dismutase
刊名: FERTILITY AND STERILITY
发表日期: 2011-07-01
DOI: 10.1016/j.fertnstert.2011.04.048
卷: 96, 期:1, 页:19-U652
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]: Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]: SPERM PROTEIN ; OXIDATIVE DAMAGE ; EXPRESSION ; SP22 ; IDENTIFICATION ; FERTILIZATION ; LOCALIZATION ; PROFILES ; EXPOSURE ; STRESS
英文摘要:

Objective: To determine whether the expression of DJ-1 protein, whose levels in spermatozoa have been reported to be highly correlated with male infertility caused by toxicants, is changed in spermatozoa of Chinese asthenozoospermia patients.

Design: DJ-1 measurement by Western blotting, quantitive ELISA, and isoelectric-focusing electrophoresis (IFE) combined with immunoblotting.

Setting: Academic medical center and research laboratories.

Patient(s): Asthenozoospermia patients (n = 113), including mild asthenozoospermia patients (n = 70) and moderate asthenozoospermia patients (n = 43), and age-matched control subjects (n = 58). Intervention(s): None.

Main Outcome Measure(s): DJ-1 in spermatozoawas determined by Western blotting and ELISA, the isoelectric point (pI) of DJ-1 by IFE combined with immunoblotting, and sperm superoxide dismutase (SOD) activity by an assay kit.

Result(s): The sperm DJ-1 concentration in moderate asthenozoospermia patients was lower than those in mild asthenozoospermia patients and control subjects. DJ-1 with a more acidic pI was increased in asthenozoospermia patients. Sperm SOD activity was decreased in asthenozoospermia patients.

Conclusion(s): DJ-1 levels are reduced in moderate asthenozoospermia patients. DJ-1 concentration is positively correlated with sperm motility and sperm SOD activity indicated by partial correlation analysis. (Fertil Steril (R) 2011;96:19-23. (C) 2011 by American Society for Reproductive Medicine.)

语种: 英语
项目资助者: State Key Laboratory of Natural and Biomimetic Drugs, Peking University, People&prime ; s Republic of China
WOS记录号: WOS:000292283000025
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/49907
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
2.Peking Univ, Dept Mol & Cellular Pharmacol, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
3.Peking Univ, Hosp 3, Dept Androl, Beijing 100191, Peoples R China

Recommended Citation:
Zhang, Zi-Yu,An, Chun-Na,Jiang, Hui,et al. Down-regulation of DJ-1 protein in the ejaculated spermatozoa from Chinese asthenozoospermia patients[J]. FERTILITY AND STERILITY,2011,96(1):19-U652.
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