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学科主题: 临床医学
题名:
An optimized HPLC/MS/MS method for quantification of excitatory amino acids in rat hippocampus and its application in brain ischemia/reperfusion research
作者: Xu, Jia1; Qian, Zhonglian2; Zhao, Libo3; Fang, Yi3
关键词: excitatory amino acids ; HPLC ; MS ; MS ; brain ischemia ; reperfusion ; rat hippocampus
刊名: BIOMEDICAL CHROMATOGRAPHY
发表日期: 2014-12-01
DOI: 10.1002/bmc.3226
卷: 28, 期:12, 页:1822-1827
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: MASS-SPECTROMETRY ; NEURONAL DEATH ; GLUTAMATE ; NIMODIPINE ; ASPARTATE ; INJURY ; GABA ; HPLC
英文摘要:

An optimized HPLC/MS/MS method was established to quantify glutamate (Glu) and aspartic acid (Asp) in rat hippocampus with glutamate-d5 (Glu-d5) as internal standard. The mass spectrometry was operated under the multiple reaction monitoring mode using electrospray ionization in the positive ion mode for Glu and negative ion mode for Asp. The retention times of Glu, Asp and Glu-d5 were 1.53, 2.07 and 1.52min, respectively. The linearity of calibration curves was good, with r(2)>0.99 and a lower limit of quantitation of 10ng/mL. The intra-day precisions (relative standard deviation, RSD) of Glu and Asp were in the range of 3.61-8.17 and 4.22-10.09%, respectively; the inter-day precisions (RSD) of Glu and Asp were in the range of 3.57-5.19 and 2.49-5.04%, respectively. The accuracies of Glu and Asp were in the range of -2.10-6.20 and -0.90-10.00%, respectively. The recovery rates of 10, 100 and 1000ng/mL were found to be 0.89 +/- 0.24, 1.01 +/- 0.10 and 0.90 +/- 0.12 for Glu and 0.99 +/- 0.26, 0.93 +/- 0.07 and 1.13 +/- 0.13 for Asp, respectively. This optimized method was successfully applied to quantify the concentration of Glu and Asp in rat hippocampus in brain ischemia/reperfusion research. Copyright (c) 2014 John Wiley & Sons, Ltd.

语种: 英语
WOS记录号: WOS:000345212600031
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/49925
Appears in Collections:北京大学第二临床医学院_药剂科_期刊论文

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作者单位: 1.Mawangdui Hosp, Dept Pharm, Changsha 410016, Hunan, Peoples R China
2.First Hosp, Dept Pharm, Hohhot 010010, Peoples R China
3.Peking Univ Peoples Hosp, Dept Pharm, Beijing 100044, Peoples R China

Recommended Citation:
Xu, Jia,Qian, Zhonglian,Zhao, Libo,et al. An optimized HPLC/MS/MS method for quantification of excitatory amino acids in rat hippocampus and its application in brain ischemia/reperfusion research[J]. BIOMEDICAL CHROMATOGRAPHY,2014,28(12):1822-1827.
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