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An optimized HPLC/MS/MS method for quantification of excitatory amino acids in rat hippocampus and its application in brain ischemia/reperfusion research
Xu, Jia1; Qian, Zhonglian2; Zhao, Libo3; Fang, Yi3
关键词Excitatory Amino Acids Hplc Ms Ms Brain Ischemia Reperfusion Rat Hippocampus
刊名BIOMEDICAL CHROMATOGRAPHY
2014-12-01
DOI10.1002/bmc.3226
28期:12页:1822-1827
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]MASS-SPECTROMETRY ; NEURONAL DEATH ; GLUTAMATE ; NIMODIPINE ; ASPARTATE ; INJURY ; GABA ; HPLC
英文摘要

An optimized HPLC/MS/MS method was established to quantify glutamate (Glu) and aspartic acid (Asp) in rat hippocampus with glutamate-d5 (Glu-d5) as internal standard. The mass spectrometry was operated under the multiple reaction monitoring mode using electrospray ionization in the positive ion mode for Glu and negative ion mode for Asp. The retention times of Glu, Asp and Glu-d5 were 1.53, 2.07 and 1.52min, respectively. The linearity of calibration curves was good, with r(2)>0.99 and a lower limit of quantitation of 10ng/mL. The intra-day precisions (relative standard deviation, RSD) of Glu and Asp were in the range of 3.61-8.17 and 4.22-10.09%, respectively; the inter-day precisions (RSD) of Glu and Asp were in the range of 3.57-5.19 and 2.49-5.04%, respectively. The accuracies of Glu and Asp were in the range of -2.10-6.20 and -0.90-10.00%, respectively. The recovery rates of 10, 100 and 1000ng/mL were found to be 0.89 +/- 0.24, 1.01 +/- 0.10 and 0.90 +/- 0.12 for Glu and 0.99 +/- 0.26, 0.93 +/- 0.07 and 1.13 +/- 0.13 for Asp, respectively. This optimized method was successfully applied to quantify the concentration of Glu and Asp in rat hippocampus in brain ischemia/reperfusion research. Copyright (c) 2014 John Wiley & Sons, Ltd.

语种英语
WOS记录号WOS:000345212600031
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/49925
专题北京大学第二临床医学院_药剂科
作者单位1.Mawangdui Hosp, Dept Pharm, Changsha 410016, Hunan, Peoples R China
2.First Hosp, Dept Pharm, Hohhot 010010, Peoples R China
3.Peking Univ Peoples Hosp, Dept Pharm, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
Xu, Jia,Qian, Zhonglian,Zhao, Libo,et al. An optimized HPLC/MS/MS method for quantification of excitatory amino acids in rat hippocampus and its application in brain ischemia/reperfusion research[J]. BIOMEDICAL CHROMATOGRAPHY,2014,28(12):1822-1827.
APA Xu, Jia,Qian, Zhonglian,Zhao, Libo,&Fang, Yi.(2014).An optimized HPLC/MS/MS method for quantification of excitatory amino acids in rat hippocampus and its application in brain ischemia/reperfusion research.BIOMEDICAL CHROMATOGRAPHY,28(12),1822-1827.
MLA Xu, Jia,et al."An optimized HPLC/MS/MS method for quantification of excitatory amino acids in rat hippocampus and its application in brain ischemia/reperfusion research".BIOMEDICAL CHROMATOGRAPHY 28.12(2014):1822-1827.
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