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Uptake of diterbium transferrin, a potential multi-photon-excited microscopy probe, into human leukemia K562 cells via a transferrin-receptor-mediated process
Yuan, Lan2; Du, Ping1; Wang, Kui1,3; Yang, Xiao-Gai1
关键词Terbium Transferrin Transferrin Receptor Uptake K562 Cells
刊名JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
2009-11-01
DOI10.1007/s00775-009-0567-8
14期:8页:1243-1251
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Chemistry, Inorganic & Nuclear
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]HUMAN-SERUM TRANSFERRIN ; DRUG-DELIVERY ; TERBIUM COMPLEXES ; BOUND IRON ; ACIDIFICATION ; LUMINESCENCE ; SWITCHES ; SENSORS ; BINDING ; CANCER
英文摘要

Our present study provided new evidence for diterbium transferrin (Tb(2)Tf) as a potential multi-photon-excited microscopy probe. It indicated that the Tb(2)Tf complex can be transported into human leukemia K562 cells via a process mediated by transferrin (Tf) receptors as an intact entity and with no obvious cellular toxicity. The supporting evidence includes the following. First, the transport kinetic behavior of Tb was compared with that of the Tf moiety. The Tb was determined by inductively coupled plasma mass spectrometry and Tf was determined by fluorescence activated cell sorting analysis. The kinetic synchronization of internalization of both Tb and Tf into human leukemia K562 cells demonstrated the Tb(2)Tf complex was transported into cells as a whole. Second, using confocal laser scanning microscopy, we observed the localization of Tb(2)Tf in the cell. This showed that the internalized Tb(2)Tf was mostly situated in the same perinuclear region as diferric transferrin (Fe(2)Tf). In addition, pretreatment with pronase largely abolished the transport process of Tb(2)Tf. The relative fluorescence intensities representing the uptake of Tf into the cells decreased to about 16% and the cytosolic Tb content decreased to almost the same percentage as for Tf. Furthermore, the addition of Fe(2)Tf can effectively inhibit transport of Tb(2)Tf into K562 cells. Third, no significant decrease of cell viability was observed in the presence of Tb(2)Tf even for 24 or 48 h by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Moreover, prospects for the use of Tb(2)Tf as a multi-photon-excited microscopy probe in a living system are discussed.

语种英语
WOS记录号WOS:000271422100009
项目编号20637010 ; 20871008
资助机构National Natural Science Foundation of China
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/49926
专题北京大学药学院_化学生物学系
北京大学医学信息学中心
作者单位1.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Med & Hlth Anal Ctr, Beijing 100191, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Natl Res Labs Nat & Biomimet Drugs, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Yuan, Lan,Du, Ping,Wang, Kui,et al. Uptake of diterbium transferrin, a potential multi-photon-excited microscopy probe, into human leukemia K562 cells via a transferrin-receptor-mediated process[J]. JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY,2009,14(8):1243-1251.
APA Yuan, Lan,Du, Ping,Wang, Kui,&Yang, Xiao-Gai.(2009).Uptake of diterbium transferrin, a potential multi-photon-excited microscopy probe, into human leukemia K562 cells via a transferrin-receptor-mediated process.JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY,14(8),1243-1251.
MLA Yuan, Lan,et al."Uptake of diterbium transferrin, a potential multi-photon-excited microscopy probe, into human leukemia K562 cells via a transferrin-receptor-mediated process".JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY 14.8(2009):1243-1251.
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