IR@PKUHSC  > 北京大学第三临床医学院
学科主题临床医学
Effects of low-level laser irradiation on proliferation and functional protein expression in human RPE cells
Dang, Yalong1,2; Wu, Wentao2; Xu, Yongsheng2,3; Mu, Yalin4; Xu, Ke4; Wu, Haotian2,5; Zhu, Yu1; Zhang, Chun2
关键词Retinal Pigment Epithelium Cells Low-level Laser Irradiation Cell Proliferation Age-related Macular Degeneration
刊名LASERS IN MEDICAL SCIENCE
2015-12-01
DOI10.1007/s10103-015-1809-3
30期:9页:2295-2302
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Engineering, Biomedical ; Surgery
资助者National Natural Science Foundation of China ; International Cooperation Foundation of Henan Province ; International Training Project for Excellent Scholars of Henan Province ; National Natural Science Foundation of China ; International Cooperation Foundation of Henan Province ; International Training Project for Excellent Scholars of Henan Province
研究领域[WOS]Engineering ; Surgery
关键词[WOS]IN-VITRO ; MACULAR DEGENERATION ; STEM-CELLS ; SUBRETINAL TRANSPLANTATION ; RATS ; DIFFERENTIATION ; VIABILITY ; MODELS ; VIVO
英文摘要

Low-level laser irradiation (LLLI) modulates a set of biological effects in many cell types such as fibroblasts, keratinocytes, and stem cells. However, no study to date has reported the effects of LLLI on retinal pigment epithelia (RPE) cells. The aim of this study was to investigate whether LLLI could enhance the proliferation of RPE cells and increase the expression of RPE functional genes/proteins. Human ARPE-19 cells were seeded overnight and treated with 8 J/cm(2) of LLLI. Cell proliferation was measured by CCK8 assay and cell cycle distribution was evaluated by FACS. The transcription of cell cycle-specific genes and RPE functional genes was quantified by RT-PCR. Moreover, the expression of ZO-1 and CRALBP were evaluated by immunostaining. A dose of 8 J/cm(2) of LLLI significantly increased proliferation and promoted cell cycle progression while upregulating the transcription of CDK4 and CCND1 and decreasing the transcription of CDKN2A, CDKN2C, and CDKN1B in human ARPE-19 cells. Additionally, LLLI enhanced the expression of ZO-1 and CRALBP in human ARPE-19 cells. In conclusion, LLLI could enhance the proliferative ability of human ARPE-19 cells by modulating cyclin D1, CDK4, and a group of cyclin-dependent kinase inhibitors. It also could increase the expression of RPE-specific proteins. Thus, LLLI may be a potential approach for the treatment of RPE degenerative diseases.

语种英语
所属项目编号81371017 ; 2015GH12 ; 2015-13
资助者National Natural Science Foundation of China ; International Cooperation Foundation of Henan Province ; International Training Project for Excellent Scholars of Henan Province ; National Natural Science Foundation of China ; International Cooperation Foundation of Henan Province ; International Training Project for Excellent Scholars of Henan Province
WOS记录号WOS:000365877200011
Citation statistics
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/49944
Collection北京大学第三临床医学院
作者单位1.Zhengzhou Univ, Dept Ophthalmol, Affiliated Hosp 1, Zhengzhou 450052, Peoples R China
2.Peking Univ, Clin Stem Cell Res Ctr, Hosp 3, Beijing 100871, Peoples R China
3.Chinese Peoples Armed Police Force, Logist Coll, Dept Biotech Treatment, Clin Lab Tissue & Cell Res Ctr, Tianjin, Peoples R China
4.Henan Univ Sci & Technol, Yellow River Hosp, Dept Ophthalmol, Sanmenxia, Peoples R China
5.Beijing 4 High Sch, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Dang, Yalong,Wu, Wentao,Xu, Yongsheng,et al. Effects of low-level laser irradiation on proliferation and functional protein expression in human RPE cells[J]. LASERS IN MEDICAL SCIENCE,2015,30(9):2295-2302.
APA Dang, Yalong.,Wu, Wentao.,Xu, Yongsheng.,Mu, Yalin.,Xu, Ke.,...&Zhang, Chun.(2015).Effects of low-level laser irradiation on proliferation and functional protein expression in human RPE cells.LASERS IN MEDICAL SCIENCE,30(9),2295-2302.
MLA Dang, Yalong,et al."Effects of low-level laser irradiation on proliferation and functional protein expression in human RPE cells".LASERS IN MEDICAL SCIENCE 30.9(2015):2295-2302.
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