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学科主题: 临床医学
题名:
Effect of aloe-emodin on expression of proliferating cell nuclear antigen of vascular smooth muscle cells in culture after arterial injury
作者: Xu, CB1; Yin, CL1; Wang, SW1
关键词: proliferating cell nuclear antigen ; aloe-emodin ; smooth muscle cell
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2001-06-01
卷: 114, 期:6, 页:571-576
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: GENE
英文摘要:

Objective To observe the effect of aloe-emodin on the expression of proliferating cell nuclear antigen (PCNA) in smooth muscle cells (SMCs) after arterial injury and study the molecular mechanism of inhibition of aloe-emodin on SMC proliferation.

Methods Deendothelialization was performed at the abdominal aorta in Japanese white rabbits using a 3F Fogarty arterial embolectomy catheter. 48 hours later, the medium of abdominal aorta was isolated and primary SMCs culture was performed. Cells were synchronized to Go by serum starvation, then aloe-emodin at a concentration of 20 mug/ml was added to the culture medium containing 10% [v/v] fetal calf serum. Vehicle was also added to the medium as a control. After 18 hours, the expression of PCNA at the level of mRNA and protein were examined using techniques of RT/PCR, Western blotting and inmmunocytochemistry respectively.

Results Compared with the control group, the expression of PCNA mRNA and protein was prominently decreased after addition of aloe-emodin.

Conclusion The inhibition of aloe-emodin on SMCs proliferation may be caused by inhibiting the expression of the PCNA gene.

语种: 中文
WOS记录号: WOS:000169654000003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/49948
Appears in Collections:北京大学第二临床医学院_心血管内科_期刊论文

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作者单位: 1.Beijing Med Univ, Peoples Hosp, Dept Cardiol, Beijing 100044, Peoples R China
2.Beijing Med Univ, Peoples Hosp, Cent Lab, Beijing 100044, Peoples R China
3.Capital Univ Med Sci, Dept Cardiol, Xuan Wu Hosp, Beijing 100053, Peoples R China

Recommended Citation:
Xu, CB,Yin, CL,Wang, SW. Effect of aloe-emodin on expression of proliferating cell nuclear antigen of vascular smooth muscle cells in culture after arterial injury[J]. CHINESE MEDICAL JOURNAL,2001,114(6):571-576.
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