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Effect of aloe-emodin on expression of proliferating cell nuclear antigen of vascular smooth muscle cells in culture after arterial injury
Xu, CB1; Yin, CL1; Wang, SW1
关键词Proliferating Cell Nuclear Antigen Aloe-emodin Smooth Muscle Cell
刊名CHINESE MEDICAL JOURNAL
2001-06-01
114期:6页:571-576
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]GENE
英文摘要

Objective To observe the effect of aloe-emodin on the expression of proliferating cell nuclear antigen (PCNA) in smooth muscle cells (SMCs) after arterial injury and study the molecular mechanism of inhibition of aloe-emodin on SMC proliferation.

Methods Deendothelialization was performed at the abdominal aorta in Japanese white rabbits using a 3F Fogarty arterial embolectomy catheter. 48 hours later, the medium of abdominal aorta was isolated and primary SMCs culture was performed. Cells were synchronized to Go by serum starvation, then aloe-emodin at a concentration of 20 mug/ml was added to the culture medium containing 10% [v/v] fetal calf serum. Vehicle was also added to the medium as a control. After 18 hours, the expression of PCNA at the level of mRNA and protein were examined using techniques of RT/PCR, Western blotting and inmmunocytochemistry respectively.

Results Compared with the control group, the expression of PCNA mRNA and protein was prominently decreased after addition of aloe-emodin.

Conclusion The inhibition of aloe-emodin on SMCs proliferation may be caused by inhibiting the expression of the PCNA gene.

语种中文
WOS记录号WOS:000169654000003
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/49948
专题北京大学第二临床医学院_心血管内科
作者单位1.Beijing Med Univ, Peoples Hosp, Dept Cardiol, Beijing 100044, Peoples R China
2.Beijing Med Univ, Peoples Hosp, Cent Lab, Beijing 100044, Peoples R China
3.Capital Univ Med Sci, Dept Cardiol, Xuan Wu Hosp, Beijing 100053, Peoples R China
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Xu, CB,Yin, CL,Wang, SW. Effect of aloe-emodin on expression of proliferating cell nuclear antigen of vascular smooth muscle cells in culture after arterial injury[J]. CHINESE MEDICAL JOURNAL,2001,114(6):571-576.
APA Xu, CB,Yin, CL,&Wang, SW.(2001).Effect of aloe-emodin on expression of proliferating cell nuclear antigen of vascular smooth muscle cells in culture after arterial injury.CHINESE MEDICAL JOURNAL,114(6),571-576.
MLA Xu, CB,et al."Effect of aloe-emodin on expression of proliferating cell nuclear antigen of vascular smooth muscle cells in culture after arterial injury".CHINESE MEDICAL JOURNAL 114.6(2001):571-576.
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