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学科主题: 口腔医学
题名:
Polymerase chain reaction-based denaturing gradient gel electrophoresis in the evaluation of oral microbiota
作者: Li, Y.1; Saxena, D.1; Barnes, V. M.1; Trivedi, H. M.1; Ge, Y.1; Xu, T.1
关键词: dental plaque ; fluoride dentifrice ; oral microbes ; polymerase chain reaction-denaturing gradient gel electrophoresis ; prophylactic treatment
刊名: ORAL MICROBIOLOGY AND IMMUNOLOGY
发表日期: 2006-10-01
卷: 21, 期:5, 页:333-339
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dentistry, Oral Surgery & Medicine ; Immunology ; Microbiology
研究领域[WOS]: Dentistry, Oral Surgery & Medicine ; Immunology ; Microbiology
关键词[WOS]: 16S RIBOSOMAL-RNA ; BACTERIAL COMMUNITY STRUCTURE ; SUPRAGINGIVAL DENTAL PLAQUE ; SUBGINGIVAL PLAQUE ; DGGE ; DIVERSITY ; VITALITY ; COUNTS ; GENES ; PCR
英文摘要:

Background/aims: Clinical evaluation of oral microbial reduction after a standard prophylactic treatment has traditionally been based on bacterial cultivation methods. However, not all microbes in saliva or dental plaque can be cultivated. Polymerase chain reaction-based denaturing gradient gel electrophoresis (PCR-DGGE) is a cultivation-independent molecular fingerprinting technique that allows the assessment of the predominant bacterial species present in the oral cavity. This study sought to evaluate the oral microbial changes that occurred after a standard prophylactic treatment with a conventional oral care product using PCR-DGGE.

Methods: Twelve healthy adults participated in the study. Pooled plaque samples were collected at baseline, 24 h after prophylaxis (T1), and 4 days after toothbrushing with fluoride toothpaste (T4). The total microbial genomic DNA of the plaque was isolated. PCR was performed with a set of universal bacterial 16S rDNA primers. The PCR-amplified 16S rDNA fragments were separated by DGGE. The effects of the treatment and of dental brushing were assessed by comparing the PCR-DGGE fingerprinting profiles.

Results: The mean numbers of detected PCR amplicons were 22.3 +/- 6.1 for the baseline group, 13.0 +/- 3.1 for the T1 group, and 13.5 +/- 4.3 for the T4 group; the differences among the three groups were statistically significant (P < 0.01). The study also found a significant difference in the mean similarities of microbial profiles between the baseline and the treatment groups (P < 0.001).

Conclusion: PCR-based DGGE has been shown to be an excellent means of rapidly and accurately assessing oral microbial changes in this clinical study.

语种: 英语
WOS记录号: WOS:000239864000010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/49949
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.NYU, Coll Dent, Dept Basic Sci & Craniofacial Biol, New York, NY 10010 USA
2.Colgate Palmolive Technol Ctr, Piscataway, NJ USA
3.Peking Univ, SCh Stomatol, Beijing 100871, Peoples R China

Recommended Citation:
Li, Y.,Saxena, D.,Barnes, V. M.,et al. Polymerase chain reaction-based denaturing gradient gel electrophoresis in the evaluation of oral microbiota[J]. ORAL MICROBIOLOGY AND IMMUNOLOGY,2006,21(5):333-339.
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