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学科主题: 临床医学
题名:
Antagonistic effect of C19 on migration of vascular smooth muscle cells and intimal hyperplasia induced by chemokine-like factor 1
作者: Chen, Feng1; Zhang, Tao1; Qiao, Zhengguo2; Zhang, Xiaoming3; Xiong, Jiang1; Jia, Xin1; Chen, Jian4; Shen, Chenyang3; Guo, Wei1
关键词: CKLF1 ; C19 ; Intimal hyperplasia ; Vascular Smooth Muscle Cell ; Migration
刊名: MOLECULAR BIOLOGY REPORTS
发表日期: 2013-04-01
DOI: 10.1007/s11033-012-2309-1
卷: 40, 期:4, 页:2939-2946
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: C-TERMINAL PEPTIDES ; CKLF1 ; RESTENOSIS ; CHEMOTAXIS ; EXPRESSION ; CYTOKINE ; CCR4
英文摘要:

A chemokine-like factor 1 (CKLF1) is a recently discovered chemokine with broad-spectrum biological functions in inflammation and autoimmune diseases. C19 as a CKLF1′s C-terminal peptide has been reported to exert inhibitory effects in a variety of diseases. However, the roles of CKLF1 and C19 on vascular smooth muscle cell (VSMC) migration and neointima formation still remain elusive. The effects of CKLF1 and C19 on VSMC migration and neointimal formation were investigated in cultured VSMCs and balloon-injured rat carotid arteries based on techniques including adenovirus-induced CKLF1 overexpression, gel based perivascular administration of C19, Boyden chamber, scratch-wound assay, real-time PCR, western blot and immunohistochemical analysis. CKLF1 was noticed to accumulate preferentially in neointima after the injury and colocalize with VSMCs. Luminal delivery of CKLF1 adenovirus to arteries exacerbated intimal thickening while perivascular administration of C19 to injured arteries attenuated this problem. In cultured primary VSMCs, CKLF1 overexpression up-regulated VSMC migration, which was down-regulated by C19. These data suggest that CKLF1 has a pivotal role in intimal hyperplasia by mediating VSMC migration. C19 was demonstrated to inhibit CKLF1-mediatated chemotaxis and restenosis. Thus further studies on C19 may provide a new treatment perspective for atherosclerosis and post-angioplasty restenosis.

语种: 英语
所属项目编号: 201150M1532
项目资助者: China Postdoctoral Science Foundation
WOS记录号: WOS:000316221100019
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/49954
Appears in Collections:北京大学第二临床医学院_中心实验室_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Dept Cent Lab, Beijing 100044, Peoples R China
2.Chinese Peoples Liberat Army Gen Hosp, Dept Vasc Surg, Clin Div Surg, Beijing 100853, Peoples R China
3.Peking Univ, Dept Vasc Surg, Peoples Hosp, Beijing 100044, Peoples R China
4.Chinese Acad Sci, State Key Lab Transducer Technol, Inst Elect, Beijing 100190, Peoples R China

Recommended Citation:
Chen, Feng,Zhang, Tao,Qiao, Zhengguo,et al. Antagonistic effect of C19 on migration of vascular smooth muscle cells and intimal hyperplasia induced by chemokine-like factor 1[J]. MOLECULAR BIOLOGY REPORTS,2013,40(4):2939-2946.
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