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学科主题: 临床医学
题名:
Increased TNF-alpha and PCI2, but not NO release from macrophages in 18-month-old rats
作者: Tang, YM1; Di Pietro, L1; Feng, Y1; Wang, X1
关键词: TNF-alpha ; prostaglandin ; nitric oxide ; macrophage
刊名: MECHANISMS OF AGEING AND DEVELOPMENT
发表日期: 2000-03-13
卷: 114, 期:2, 页:79-88
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology ; Geriatrics & Gerontology
研究领域[WOS]: Cell Biology ; Geriatrics & Gerontology
关键词[WOS]: TUMOR-NECROSIS-FACTOR ; MOUSE PERITONEAL-MACROPHAGES ; GENE-RELATED PEPTIDE ; NITRIC-OXIDE PRODUCTION ; SUPEROXIDE ANION ; SENESCENT MICE ; AGE ; SECRETION ; INTERLEUKIN-6 ; EXPRESSION
英文摘要:

TNF-alpha is an important pro-inflammatory mediator that influences host defense against infection and cancer. Previous examinations of TNF-alpha release and synthesis within the context of age have provided conflicting data, as both increased and decreased TNF-alpha synthesis have been described in aged populations. The present study was designed to reevaluate TNF-alpha production and synthesis in primary cultured peritoneal macrophages of young and 18-month-old rats. We were also interested in the link between the production of this cytokine and other important mediators, such as prostaglandin I-2 (PGI(2)) and nitric oxide (NO) in these rats, Primary cultured peritoneal macrophages of rat were stimulated with 1.0 mu g/ml of lipopolysacharide (LPS) for 12 h. The level of TNF-alpha protein in culture supernatant was measured by enzyme-linked-immunosorbent-assay (ELISA), and TNF-alpha mRNA production was assessed by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). At the same time, the levels of NO and PGI(2) were measured, Macrophages from 18-month-old rats produced more TNF-alpha protein, PGI(2) and TNF-alpha mRNA than those From the young rats (2 month). There was no difference in NO production of macrophages between 18-month-old and young rats. The results demonstrate that TNF-alpha and PGI(2) production by rat macrophages increase with age. The results also suggest that NO might not contribute to the increased TNF-alpha production in 18-month-old rat macrophages. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

语种: 英语
WOS记录号: WOS:000087160900002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/49969
Appears in Collections:北京大学第三临床医学院_心血管内科_期刊论文

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作者单位: 1.Beijing Med Univ, Hosp 3, Inst Vasc Med, Beijing 100083, Peoples R China
2.Loyola Univ, Med Ctr, Dept Surg, Maywood, IL 60153 USA

Recommended Citation:
Tang, YM,Di Pietro, L,Feng, Y,et al. Increased TNF-alpha and PCI2, but not NO release from macrophages in 18-month-old rats[J]. MECHANISMS OF AGEING AND DEVELOPMENT,2000,114(2):79-88.
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