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学科主题: 基础医学
题名:
Cartilage-derived morphogenetic protein-1 promotes the differentiation of mesenchymal stem cells into chondrocytes
作者: Bai, XW1; Xia, ZF1; Pan, YQ1; Hu, J1; Pohl, J1; Wen, JH1; Li, LS1
关键词: mesenchymal stem cells ; transforming growth factor ; cartilage-derived morphogenetic protein ; chondrogenesis ; chondrocytes ; differentiation
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2004-12-10
DOI: 10.1016/j.bbrc.2004.10.055
卷: 325, 期:2, 页:453-460
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: TGF-BETA-SUPERFAMILY ; GROWTH/DIFFERENTIATION FACTOR-5 ; ARTICULAR-CARTILAGE ; BONE-MARROW ; CHONDROGENIC DIFFERENTIATION ; SKELETAL DEVELOPMENT ; PROGENITOR CELLS ; ATDC5 CELLS ; CDMP1 GENE ; MOUSE LIMB
英文摘要:

Mesenchymal stem cells (MSCs) are able to differentiate into many types of cells including chondrocytes. Transforming growth factor betal (TGF-betal) is very important in the regulation of chondrogenesis. Since cartilage-derived morphogenetic protein-1 (CDMP-1) belongs to the TGF-beta superfarnily, we tested whether CDMP-1 plays any role in the regulation of the differentiation of MSCs into chondrocytes using a high density pellet culture system. Based on the histological staining of glycosaminogly can using toluidine blue dye-binding method we found that CDMP-1 could initiate chondrogenic differentiation of MSCs as did TGF-betal. However. CDMP-1 was less stimulatory than TGF-betal. The combination of CDMP-1 and TGF-betal synergically induced chondrogenesis of MSCs. This synergic chondrogenic effect of CDMP-1 together with TGF-betal was further confirmed by quantification of GAG using dimethylinethylene blue dye-binding assay and immunohistochemical analysis of the expression of cartilage-specific protein collagen II. This study may provide an improved induction approach using MSCs for repairing damaged cartilage. (C) 2004 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000225279600013
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/49971
Appears in Collections:基础医学院_北京大学干细胞研究中心_期刊论文

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作者单位: 1.Peking Univ, Ctr Hlth Sci, Stem Cell Res Ctr, Beijing 100083, Peoples R China
2.Peking Univ, Ctr Hlth Sci, Med & Hlth Anal Ctr, Beijing 100083, Peoples R China
3.Biopharm GMBH, D-69115 Heidelberg, Germany

Recommended Citation:
Bai, XW,Xia, ZF,Pan, YQ,et al. Cartilage-derived morphogenetic protein-1 promotes the differentiation of mesenchymal stem cells into chondrocytes[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2004,325(2):453-460.
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