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学科主题: 临床医学
题名:
Rho-associated protein kinase inhibitor, Y-27632, significantly enhances cell adhesion and induces a delay in G(1) to S phase transition in rabbit corneal endothelial cells
作者: Hong, Jing; Diao, Yu-Mei
关键词: Y-27632 ; corneal endothelial cells ; proliferation ; cell cycle
刊名: MOLECULAR MEDICINE REPORTS
发表日期: 2015-08-01
DOI: 10.3892/mmr.2015.3628
卷: 12, 期:2, 页:1951-1956
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Medicine, Research & Experimental
研究领域[WOS]: Oncology ; Research & Experimental Medicine
关键词[WOS]: LARGE T-ANTIGEN ; ROCK INHIBITOR ; OLDER DONORS ; SERINE/THREONINE KINASE ; PROLIFERATION ; MIGRATION ; BINDING ; EXPRESSION ; DYSTROPHY ; CULTURE
英文摘要:

Human corneal endothelial cells are a non-prolif-erative cell type. As a result of the increase in corneal endothelium disease, increasing numbers of studies have been conducted in order to promote corneal endothelial cell proliferation. The aim of the present study was to investigate the proliferative effects of Rho-associated protein kinase inhibitor, Y-27632, on rabbit corneal endothelial cells (rCECs). Y-27632 (1, 10 or 30 mu M) was added at two different time points to two groups of rCECs. The first group received Y-27632 when rCECs were initially plated, and the second following 72 h of cell growth. Cell morphology and cell adhesion ratios were subsequently observed using light microscopy. A cell counting kit was used to measure the number of viable cells that adhered to culture plates. Cell cycle transitions and levels of Annexin V-positive apoptotic cells were detected using flow cytometry. Cells treated with 1 mu M Y-27632 and 10 mu M Y-27632 retained their cell shape. At a concentration of 30 mu M Y-27632, the cell shape became irregular. Cell adhesion ratios, in 1 mu M Y-27632 (36.84%), 10 mu M Y-27632 (84.21%) and 30 mu M Y-27632 (84.21%) were higher than the adhesion ratio in the control group (P<0.01). The optical densities of rCECs treated with 10 mu M or 30 mu M Y-27632 following 72 h of cell growth was less than that of the control cells (P<0.01), but higher than that of cells which received Y-27632 at the time of plating (P<0.01). Flow cytometry results also demonstrated that there was a delay in G(1) to S phase cell cycle progression in rCECs following administration of 10 mu M Y-27632 (P<0.01). Cell apoptosis was inhibited when 10 mu M Y-27632 was added, at the time of cell plating, as well as when added following 72 h of cell growth (P<0.01). At a concentration of 10 mu M Y-27632, there was an improvement in cell adhesion and an inhibition of the cell cycle in rabbit corneal endothelial cells. In conclusion, Y-27632 has different effects on rCECs when administered at varying concentrations and at particular stages of cell growth.

语种: 英语
所属项目编号: 31271045
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000358678600046
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/49975
Appears in Collections:北京大学第三临床医学院_眼科_期刊论文

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作者单位: Peking Univ, Hosp 3, Dept Ophthalmol, Beijing 100191, Peoples R China

Recommended Citation:
Hong, Jing,Diao, Yu-Mei. Rho-associated protein kinase inhibitor, Y-27632, significantly enhances cell adhesion and induces a delay in G(1) to S phase transition in rabbit corneal endothelial cells[J]. MOLECULAR MEDICINE REPORTS,2015,12(2):1951-1956.
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