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学科主题: 口腔医学
题名:
Aquaporin-1 and aquaporin-3 expressions in the temporomandibular joint condylar cartilage after an experimentally induced osteoarthritis
作者: Meng Juan-hong1,2; Ma Xu-chen1; Li Zhi-min1; Wu Deng-cheng3
关键词: aquaporin ; osteoarthritis ; temporomandibular joint
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2007-12-20
卷: 120, 期:24, 页:2191-2194
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: WATER CHANNELS ; ARTICULAR CHONDROCYTES ; BIOCHEMISTRY ; METABOLISM ; MICE ; AQP3
英文摘要:

Background Over 70% of the total tissue weight in the cartilage matrix consists of water, and the early-stage osteoarthritic cartilage is characterized by swelling. Water transport in the cartilage matrix and across the membranes of chondrocytes may be important in normal and pathological conditions of cartilage. The purpose of this study was to identify aquaporin-1 (AQP1) and aquaporin-3 (AQP3) expressions in the mandibular condylar cartilage after experimentally induced osteoarthritis (OA) in rats.

Methods An experimental temporomandibular joint OA was induced by partial discectomy in rats. The pathological characteristics of the normal, early-stage, and late-stage osteoarthritic TMJ cartilages were verified by histological techniques. The AQP1 and AQP3 gene expressions in the normal and osteoarthritic cartilages were measured using quantitative real-time reverse-transcription PCR analysis. The cartilage sections were incubated in primary polyclonal antibodies to AQP3; immunofluorescent microscopy was used to examine the AQP3 expression shown by its protein level.

Results The mRNA expression levels of AQP1 and AQP3, analyzed using quantitative PCR, revealed that AQP3 mRNA was highly up-regulated in the CA cartilage, which was considered significant. There was no notable difference in the expression of AQP1 mRNA between OA and normal controls. With the progressing of the OA, the localization of the AQP3 protein was quite different from that of the normal cartilage. Compared to the normal cartilage, the expressions of AQP3 protein were observed mainly in the proliferative zone and the upper mid-zone chondrocytes at the early-stage of OA, and were observed to appear frequently throughout the mid- and deep zone during the late-stage of OA. Conclusions The high expression of AQP3 mRNA in the OA cartilage and the different localization of the AQP3 protein suggest that it may play a particular role in OA pathogenesis. Further study of AQP3 function may provide new insight into the understanding of the molecular mechanisms underlying OA.

语种: 英语
WOS记录号: WOS:000251997400006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/49979
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Peking Univ, Sch Stomatol, Ctr TMD & Orofacial Pain, Beijing 100081, Peoples R China
2.Peking Univ, Sch Stomatol, Dept Oral & Maxillofacial Surg, Beijing 100081, Peoples R China
3.Peking Univ, Sch Stomatol, Lab Oral & Maxillofacial Surg, Beijing 100081, Peoples R China

Recommended Citation:
Meng Juan-hong,Ma Xu-chen,Li Zhi-min,et al. Aquaporin-1 and aquaporin-3 expressions in the temporomandibular joint condylar cartilage after an experimentally induced osteoarthritis[J]. CHINESE MEDICAL JOURNAL,2007,120(24):2191-2194.
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