北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第二临床医学院  > 期刊论文
学科主题: 临床医学
题名:
Extended effects of human papillomavirus 16 E6-specific short hairpin RNA on cervical carcinoma cells
作者: Bai, L1; Wei, L1; Wang, J1; Li, X1; He, P1
关键词: cervical carcinoma ; human papillomavirus ; short hairpin RNA
刊名: INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER
发表日期: 2006-03-01
卷: 16, 期:2, 页:718-729
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Obstetrics & Gynecology
研究领域[WOS]: Oncology ; Obstetrics & Gynecology
关键词[WOS]: HUMAN-PAPILLOMAVIRUS TYPE-16 ; RETINOBLASTOMA GENE-PRODUCT ; SHORT INTERFERING RNAS ; HEPATITIS-B-VIRUS ; EPITHELIAL-CELLS ; MAMMALIAN-CELLS ; MESSENGER-RNA ; CANCER-CELLS ; EXPRESSION ; E6
英文摘要:

Most cervical carcinomas express high-risk human papillomavirus (HPV) E6 and E7 oncogenes. Small interfering RNA can mediate sequence-specific inhibition of gene expression in mammalian cells. To find a most effective short hairpin RNA (shRNA) for HPV16 E6 messenger RNA (mRNA) and investigate the extended effects of the HPV16 E6 shRNA on cervical carcinoma cells, we stably transfected SiHa cells with four shRNA expression vectors (E6A-D). HPV16 E6A shRNA was found to be the most efficient in our study, which caused the reduction of HPV16 E6 mRNA to 10% in SiHa cells but did not reduce HPV18 E6 mRNA expression in HeLa cells. We subsequently demonstrated that E6A could stably express shRNA and effectively reduce HPV16 E6 and E7 viral genes expression in SiHa cells for more than 4 months. After E6 and E7 repression, there was a dramatic accumulation of p53, p21, and hypophosphorylated pRb proteins in cells. Furthermore, cell proliferation, colony formation ability, tumorigenicity, and in vitro cell invasive capability were suppressed substantially in E6A-transfected cells. These results suggest that the use of shRNA expression vector may be a potential approach for the treatment of persistent HPV infection and HPV-positive cervical carcinoma.

语种: 英语
WOS记录号: WOS:000236943200041
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/49982
Appears in Collections:北京大学第二临床医学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Peoples Hosp, Dept Obstet & Gynecol, Beijing 100044, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Mol Biol, Beijing 100871, Peoples R China

Recommended Citation:
Bai, L,Wei, L,Wang, J,et al. Extended effects of human papillomavirus 16 E6-specific short hairpin RNA on cervical carcinoma cells[J]. INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER,2006,16(2):718-729.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Bai, L]'s Articles
[Wei, L]'s Articles
[Wang, J]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Bai, L]‘s Articles
[Wei, L]‘s Articles
[Wang, J]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace