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Extended effects of human papillomavirus 16 E6-specific short hairpin RNA on cervical carcinoma cells
Bai, L1; Wei, L1; Wang, J1; Li, X1; He, P1
关键词Cervical Carcinoma Human Papillomavirus Short Hairpin Rna
刊名INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER
2006-03-01
16期:2页:718-729
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Obstetrics & Gynecology
研究领域[WOS]Oncology ; Obstetrics & Gynecology
关键词[WOS]HUMAN-PAPILLOMAVIRUS TYPE-16 ; RETINOBLASTOMA GENE-PRODUCT ; SHORT INTERFERING RNAS ; HEPATITIS-B-VIRUS ; EPITHELIAL-CELLS ; MAMMALIAN-CELLS ; MESSENGER-RNA ; CANCER-CELLS ; EXPRESSION ; E6
英文摘要

Most cervical carcinomas express high-risk human papillomavirus (HPV) E6 and E7 oncogenes. Small interfering RNA can mediate sequence-specific inhibition of gene expression in mammalian cells. To find a most effective short hairpin RNA (shRNA) for HPV16 E6 messenger RNA (mRNA) and investigate the extended effects of the HPV16 E6 shRNA on cervical carcinoma cells, we stably transfected SiHa cells with four shRNA expression vectors (E6A-D). HPV16 E6A shRNA was found to be the most efficient in our study, which caused the reduction of HPV16 E6 mRNA to 10% in SiHa cells but did not reduce HPV18 E6 mRNA expression in HeLa cells. We subsequently demonstrated that E6A could stably express shRNA and effectively reduce HPV16 E6 and E7 viral genes expression in SiHa cells for more than 4 months. After E6 and E7 repression, there was a dramatic accumulation of p53, p21, and hypophosphorylated pRb proteins in cells. Furthermore, cell proliferation, colony formation ability, tumorigenicity, and in vitro cell invasive capability were suppressed substantially in E6A-transfected cells. These results suggest that the use of shRNA expression vector may be a potential approach for the treatment of persistent HPV infection and HPV-positive cervical carcinoma.

语种英语
WOS记录号WOS:000236943200041
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被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/49982
专题北京大学第二临床医学院
北京大学第三临床医学院_超声影像科
作者单位1.Peking Univ, Peoples Hosp, Dept Obstet & Gynecol, Beijing 100044, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Mol Biol, Beijing 100871, Peoples R China
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GB/T 7714
Bai, L,Wei, L,Wang, J,et al. Extended effects of human papillomavirus 16 E6-specific short hairpin RNA on cervical carcinoma cells[J]. INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER,2006,16(2):718-729.
APA Bai, L,Wei, L,Wang, J,Li, X,&He, P.(2006).Extended effects of human papillomavirus 16 E6-specific short hairpin RNA on cervical carcinoma cells.INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER,16(2),718-729.
MLA Bai, L,et al."Extended effects of human papillomavirus 16 E6-specific short hairpin RNA on cervical carcinoma cells".INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER 16.2(2006):718-729.
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