学科主题临床医学
Identification of novel molecular candidates for acute liver failure in plasma of BALB/c murine model
Lv, Sa1; Wei, Lai1; Wang, Jiang-hua1; Wang, Jing-yan1; Liu, Feng1
关键词Proteomics Phosphatidylethanolamine Binding Protein D-galactosamine Lipopolysaccharide Two-dimensional Electrophoresis
刊名JOURNAL OF PROTEOME RESEARCH
2007
DOI10.1021/pr0701759
6期:7页:2746-2752
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]PHOSPHATIDYLETHANOLAMINE-BINDING-PROTEIN ; CHOLINERGIC NEUROSTIMULATING PEPTIDE ; SEQUENCE HOMOLOGY ; PRECURSOR PROTEIN ; SERUM PROTEOME ; MESSENGER-RNA ; UNITED-STATES ; EXPRESSION ; RAT ; BRAIN
英文摘要

In an effort to identify proteins involved in the disease process of acute liver failure (ALF), we investigated changes in the plasma proteome associated with D-galactosamine/lipopolysaccharide (GalN/LPS) treatment of BALB/c mice. The plasma samples from mice with ALF and control were screened for potential differences using two-dimensional electrophoresis followed by liquid chromatography-electrospray ionization-tandem mass spectrometry or matrix associated laser desorption ionization-time-of-flight mass spectrometry. The expression levels of candidate protein named phosphatidylethanolamine binding protein (PEBP) in plasma and liver, brain tissues were confirmed by western blot and RT-PCR analyses. Results were confirmed in plasma samples of human beings. Seven proteins existed in plasma of GalN/LPS-treatment animals only but not in controls. They included PEBP, regucalcin, Cu/Zn superoxide dismutase, glyoxalase 1, malate dehydrogenase, proteasome subunit alpha type 1, and HPMS haptoglobin precursor. Two proteins, proteasome subunit alpha type 5 and apolipoprotein A-I precursor, were up-regulated by GalN/LPS, and one protein, HPMS haptoglobin precursor, was down-regulated by this treatment. Western blot analysis confirmed the results that PEBP protein levels increased significantly in plasma and liver tissues only in ALF mice, but not in surviving mice treated with GalN/LPS. Further analysis revealed that GalN/LPS also induced up-regulation of PEBP mRNA levels in liver tissues. Importantly, plasma obtained from ALF patients, but not from healthy volunteers or from hepatitis patients, also contained detectable levels of PEBP. The present study show that PEBP may be a potential plasma biomarker for ALF diagnosis and participate in the pathphysiological process of ALF.

语种英语
WOS记录号WOS:000247792300034
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被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/49990
专题北京大学第二临床医学院_北京大学肝病研究所
作者单位1.Peking Univ, Peking Univ Peoples Hosp, Inst Hepatol, Beijing 100044, Peoples R China
2.Second Affiliated Hosp China Med Univ, Dept Infect Dis, Shenyang 110004, Peoples R China
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Lv, Sa,Wei, Lai,Wang, Jiang-hua,et al. Identification of novel molecular candidates for acute liver failure in plasma of BALB/c murine model[J]. JOURNAL OF PROTEOME RESEARCH,2007,6(7):2746-2752.
APA Lv, Sa,Wei, Lai,Wang, Jiang-hua,Wang, Jing-yan,&Liu, Feng.(2007).Identification of novel molecular candidates for acute liver failure in plasma of BALB/c murine model.JOURNAL OF PROTEOME RESEARCH,6(7),2746-2752.
MLA Lv, Sa,et al."Identification of novel molecular candidates for acute liver failure in plasma of BALB/c murine model".JOURNAL OF PROTEOME RESEARCH 6.7(2007):2746-2752.
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