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学科主题: 基础医学
题名:
SOX17 antagonizes WNT/beta-catenin signaling pathway in hepatocellular carcinoma
作者: Jia, Yan1,2; Yang, Yunsheng1; Liu, Shuang3; Herman, James G.4; Lu, Fengmin5; Guo, Mingzhou1
关键词: DNA methylation ; hepatocellular carcinoma ; SOX17 ; 5-aza-2 &prime ; -deoxycytidine ; WNT signaling pathway
刊名: EPIGENETICS
发表日期: 2010-11-01
DOI: 10.4161/epi.5.8.13104
卷: 5, 期:8, 页:743-749
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: BETA-CATENIN ; COLORECTAL-CANCER ; STEM-CELLS ; EPIGENETIC INACTIVATION ; MOLECULAR PATHOGENESIS ; COLON-CARCINOMA ; ALPHA-CATENIN ; EXPRESSION ; PROTEINS ; DISEASE
英文摘要:

SRY-box containing gene 17 (SOX17) was reported to be indispensable for embryonic development and a candidate tumor suppressor gene which antagonizes the canonical WNT/beta-catenin signaling pathway in colorectal cancer. In this study, we investigated the function and epigenetic regulation of SOX17 in human hepatocellular carcinoma (HCC). DNA methylation of SOX17 was analyzed in 62 human HCC tissues and HCC cell lines by MSP. A role as a tumor suppressor gene was evaluated by colony formation assay and regulation of WNT/beta-catenin signal pathway by SOX17 was determined by IHC and luciferase reporter assay. DNA methylation of the SOX17 promoter region occurs in 82% of HCC tissues and is associated with nuclear accumulation of beta-catenin. Restoration of SOX17 inhibits HepG2 colony formation and beta-catenin/TCF-dependent transcription with the presence of HMG box in SOX17. In conclusion, SOX17 negatively regulates canonical WNT/beta-catenin signaling pathway and inhibits human HCC cells growth, providing an explanation for the loss of expression by epigenetic mechanisms in these tumors.

语种: 英语
所属项目编号: 2010CB912802 ; 2009ZX10004-903
项目资助者: National Basic Research Program (973 Program) ; National S& ; T Major Project for Infections Control
WOS记录号: WOS:000284263500010
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/49998
Appears in Collections:基础医学院_病原生物学系_期刊论文

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作者单位: 1.Chinese Peoples Liberat Army Gen Hosp, Dept Gastroenterol & Hepatol, Beijing, Peoples R China
2.Nankai Univ, Coll Med, Tianjin 300071, Peoples R China
3.Capital Med Univ, Beijing Youan Hosp, Beijing, Peoples R China
4.Sidney Kimmel Comprehens Canc Ctr Johns Hopkins, Baltimore, MD USA
5.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100871, Peoples R China

Recommended Citation:
Jia, Yan,Yang, Yunsheng,Liu, Shuang,et al. SOX17 antagonizes WNT/beta-catenin signaling pathway in hepatocellular carcinoma[J]. EPIGENETICS,2010,5(8):743-749.
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