IR@PKUHSC  > 北京大学第一临床医学院  > 皮肤性病科
学科主题临床医学
The A395T Mutation in ERG11 Gene Confers Fluconazole Resistance in Candida tropicalis Causing Candidemia
Tan, Jingwen1,2; Zhang, Jinqing1,2; Chen, Wei1,2; Sun, Yi1,2; Wan, Zhe1,2; Li, Ruoyu1,2; Liu, Wei1,2
关键词Candida Tropicalis Fluconazole Resistance Erg11 Three-dimensional Model
刊名MYCOPATHOLOGIA
2015-04-01
DOI10.1007/s11046-014-9831-8
179期:3-4页:213-218
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Mycology
资助者Program for New Century Excellent Talents in University ; National Natural Science Foundation of China ; Program for New Century Excellent Talents in University ; National Natural Science Foundation of China
研究领域[WOS]Mycology
关键词[WOS]AZOLE ANTIFUNGAL AGENTS ; LANOSTEROL 14-ALPHA-DEMETHYLASE ; STEROL 14-ALPHA-DEMETHYLASE ; DRUG-RESISTANCE ; WILD-TYPE ; ALBICANS ; SURVEILLANCE ; MECHANISMS ; CYP51 ; SUSCEPTIBILITIES
英文摘要

The mechanism of fluconazole resistance in Candida tropicalis is still unclear. Recently, we isolated a fluconazole-resistant strain of C. tropicalis from the blood specimen of a patient with candidemia in China. In vitro antifungal susceptibility of the isolate was determined by using CLSI M27-A3 and E-test methods. The sequence of ERG11 gene was then analyzed, and the three-dimensional model of Erg11p encoded by ERG11 gene was also investigated. The sequencing of ERG11 gene revealed the mutation of A395T in this fluconazole-resistant isolate of C. tropicalis, resulting in the Y132F substitution in Erg11p. Sequence alignment and three-dimensional model comparison of Erg11ps showed high similarity between fluconazole-susceptible isolates of C. tropicalis and Candida albicans. The comparison of the three-dimensional models of Erg11ps demonstrated that the position of the Y132F substitution in this isolate of C. tropicalis is identical to the isolate of C. albicans with fluconazole resistance resulting from Y132F substitution in Erg11p. Hence, we ascertain that the Y132F substitution of Erg11p caused by A395T mutation in ERG11 gene confers the fluconazole resistance in C. tropicalis.

语种英语
所属项目编号NCET-10-0198 ; BMU20110158 ; 81471925
资助者Program for New Century Excellent Talents in University ; National Natural Science Foundation of China ; Program for New Century Excellent Talents in University ; National Natural Science Foundation of China
WOS记录号WOS:000351168800005
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/49999
Collection北京大学第一临床医学院_皮肤性病科
作者单位1.Peking Univ, Hosp 1, Dept Dermatol, Ctr Med Mycol, Beijing 100034, Peoples R China
2.Peking Univ, Hosp 1, Dept Dermatol & Venereol, Res Ctr Med Mycol, Beijing 100034, Peoples R China
Recommended Citation
GB/T 7714
Tan, Jingwen,Zhang, Jinqing,Chen, Wei,et al. The A395T Mutation in ERG11 Gene Confers Fluconazole Resistance in Candida tropicalis Causing Candidemia[J]. MYCOPATHOLOGIA,2015,179(3-4):213-218.
APA Tan, Jingwen.,Zhang, Jinqing.,Chen, Wei.,Sun, Yi.,Wan, Zhe.,...&Liu, Wei.(2015).The A395T Mutation in ERG11 Gene Confers Fluconazole Resistance in Candida tropicalis Causing Candidemia.MYCOPATHOLOGIA,179(3-4),213-218.
MLA Tan, Jingwen,et al."The A395T Mutation in ERG11 Gene Confers Fluconazole Resistance in Candida tropicalis Causing Candidemia".MYCOPATHOLOGIA 179.3-4(2015):213-218.
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