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学科主题: 临床医学
题名:
Improved peripheral nerve regeneration with sustained release nerve growth factor microspheres in small gap tubulization
作者: Wang, Zhenwei1; Han, Na1; Wang, Jiancheng2; Zheng, Hua2; Peng, Jianping3; Kou, Yuhui1; Xu, Chungui1; An, Shuai1; Yin, Xiaofeng1; Zhang, Peixun1; Jiang, Baoguo1
关键词: Nerve growth factor (NGF) ; microsphere ; nerve regeneration ; small gap ; tubulization
刊名: AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
发表日期: 2014
卷: 6, 期:4, 页:413-421
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Medicine, Research & Experimental
研究领域[WOS]: Oncology ; Research & Experimental Medicine
关键词[WOS]: ADULT SENSORY NEURONS ; RAT SPINAL-CORD ; NEUROTROPHIC FACTORS ; CELL-DEATH ; INJURY ; CONDUIT ; SCHWANN ; MOTOR ; SURVIVAL ; GRAFTS
英文摘要:

Objective: To evaluate the long-term results of the use of nerve growth factor (NGF)-loaded poly-D, L-lactide- co-glycolide (PLGA) microspheres for improve nerve regeneration with small gap tubulization. Methods: NGF microspheres were prepared by a modified W/O/W emulsion solvent evaporation method. Forty-eight male SD rats were separated into 4 groups and received a chitin conduit to bridge a sciatic nerve injury left a 2 mm gap. Saline (Group A), 20 ng/ml NGF solution (Group B), blank PLGA microspheres (Group C), or 40 ng/ml NGF-loaded microspheres (Group D) was injected in the gap. Each group had two study endpoints, 3 months subgroup and 1 year subgroup. Results: The myelinated fiber count at 2 mm distal to the conduit at 1 year was slightly less than at 3 months in all groups (P>0.05). However, the maturity of the myelinated nerves at 1 year was obviously improved. The fiber count, myelin sheath thickness, axon area of NGF microsphere group were significantly higher than the saline groups at 3 months (P=0.05, P<0.05, and P<0.05, respectively). The SFI was significantly improved in NGF microspheres group compared to the saline group and NGF solution group at 1 year (P<0.05, and P<0.05, respectively). Conclusions: The results demonstrated that the release of NGF microspheres in small gap tubulization benefit on peripheral nerve injury facilitated nerve regeneration histologically, especially for the maturity of early regenerative nerve fibers and also had an effect on functional recovery in the long term.

语种: 英语
所属项目编号: 2014CB542200 ; IRT1201 ; 31271284 ; 31171150 ; 81171146 ; 30971526 ; 31100860 ; 31040043 ; BMU20110270
项目资助者: Chinese National Ministry of Science and Technology 973 Project Planning ; ministry of education innovation team ; National Natural Science Fund ; Educational Ministry New Century Excellent Talents Support Project
WOS记录号: WOS:000341268500009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50001
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Dept Pharmaceut, Beijing 100044, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Orthoped & Trauma, Beijing 100044, Peoples R China
3.Shanghai Jiao Tong Univ, Sch Med, Xin Hua Hosp, Dept Orthoped, Shanghai 200030, Peoples R China

Recommended Citation:
Wang, Zhenwei,Han, Na,Wang, Jiancheng,et al. Improved peripheral nerve regeneration with sustained release nerve growth factor microspheres in small gap tubulization[J]. AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH,2014,6(4):413-421.
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