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Isolation and enrichment of PC-3 prostate cancer stem-like cells using MACS and serum-free medium
Sheng, Xia1; Li, Zeng1; Wang, De-Lin1; Li, Wen-Bin1; Lu, Zhao1; Chen, Ke-Hong1; Cao, Jian-Jia1; Yu, Chao2; Liu, Wu-Jiang3
关键词Prostate Cancer Stem Cell Pc-3 Cell Cd133 Cd44 Magnetic Bead Cell Sorting Serum-free Medium
刊名ONCOLOGY LETTERS
2013-03-01
DOI10.3892/ol.2012.1090
5期:3页:787-792
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]TUMOR-INITIATING CELLS ; CARCINOMA ; BETA ; PC3 ; TUMORIGENESIS ; EXPRESSION ; SYSTEM ; GROWTH
英文摘要

Prostate cancer stem-like cells (PCSLCs) are considered to be the ′seed′ of prostate cancer. The aim of this study was to confirm that the PC-3 cells, which we isolated and enriched from PC-3 cells through magnetic bead cell sorting (MACS) and serum-free medium (SFM) culture, were PCSLCs. Combinations of MACS, flow cytometry (FCM), SFM and immunocytochemistry (ICC) were used to ensure the positive expression of CD133 and CD44 on PC-3 and sphere-forming cell membranes. Self-renewal, multi-potential differentiation, unlimited proliferation and permanency assays were also applied to indentify whether the PC-3 cells exhibited the characteristics of cancer stem cells (CSCs). As a result, there was a low proportion of PCSLCs in the PC-3 cells. In the FCM assay, the proportion of cells expressing CD133 or CD44 in the PC-3 cells was 0.51 and 0.31%, respectively. In addition, we found that the proportion of PC-3 cells sorted by MACS that expressed CD133 was significantly increased compared with that of the sphere-forming cells cultured in SFM (99.09 vs. 84.80%, P<0.05), while no difference was observed in the proportion of cells expressing CD44 between them (99.88 vs. 99.82%, P>0.05). The expression of PAP and AR as detected by western blot analysis of induced PCSLCs was significantly increased compared with that of uninduced PCSLCs (P<0.05); the proliferation capacity of PCSLCs was significantly higher than that of both the PC-3 cells (P<0.05) and induced PCSLCs (P<0.05). Furthermore, the PCSLCs that were isolated from SFM and MACS both demonstrated certain characteristics of stem cells and should be considered as stem cell-like. These data may hold potential for further exploring the role of PCSLCs.

语种英语
WOS记录号WOS:000315423900011
项目编号30972999
资助机构Natural Science Foundation of China (NSFC)
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50015
专题北京大学第一临床医学院_泌尿外科
作者单位1.Chongqing Med Univ, Affiliated Hosp 1, Dept Urol, Chongqing 400016, Peoples R China
2.Chongqing Med Univ, Life Sci Res Inst, Chongqing 400016, Peoples R China
3.Peking Univ, Inst Urol, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Sheng, Xia,Li, Zeng,Wang, De-Lin,et al. Isolation and enrichment of PC-3 prostate cancer stem-like cells using MACS and serum-free medium[J]. ONCOLOGY LETTERS,2013,5(3):787-792.
APA Sheng, Xia.,Li, Zeng.,Wang, De-Lin.,Li, Wen-Bin.,Lu, Zhao.,...&Liu, Wu-Jiang.(2013).Isolation and enrichment of PC-3 prostate cancer stem-like cells using MACS and serum-free medium.ONCOLOGY LETTERS,5(3),787-792.
MLA Sheng, Xia,et al."Isolation and enrichment of PC-3 prostate cancer stem-like cells using MACS and serum-free medium".ONCOLOGY LETTERS 5.3(2013):787-792.
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