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学科主题: 临床医学
题名:
Isolation and enrichment of PC-3 prostate cancer stem-like cells using MACS and serum-free medium
作者: Sheng, Xia1; Li, Zeng1; Wang, De-Lin1; Li, Wen-Bin1; Lu, Zhao1; Chen, Ke-Hong1; Cao, Jian-Jia1; Yu, Chao2; Liu, Wu-Jiang3
关键词: prostate cancer ; stem cell ; PC-3 cell ; CD133 ; CD44 ; magnetic bead cell sorting ; serum-free medium
刊名: ONCOLOGY LETTERS
发表日期: 2013-03-01
DOI: 10.3892/ol.2012.1090
卷: 5, 期:3, 页:787-792
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: TUMOR-INITIATING CELLS ; CARCINOMA ; BETA ; PC3 ; TUMORIGENESIS ; EXPRESSION ; SYSTEM ; GROWTH
英文摘要:

Prostate cancer stem-like cells (PCSLCs) are considered to be the ′seed′ of prostate cancer. The aim of this study was to confirm that the PC-3 cells, which we isolated and enriched from PC-3 cells through magnetic bead cell sorting (MACS) and serum-free medium (SFM) culture, were PCSLCs. Combinations of MACS, flow cytometry (FCM), SFM and immunocytochemistry (ICC) were used to ensure the positive expression of CD133 and CD44 on PC-3 and sphere-forming cell membranes. Self-renewal, multi-potential differentiation, unlimited proliferation and permanency assays were also applied to indentify whether the PC-3 cells exhibited the characteristics of cancer stem cells (CSCs). As a result, there was a low proportion of PCSLCs in the PC-3 cells. In the FCM assay, the proportion of cells expressing CD133 or CD44 in the PC-3 cells was 0.51 and 0.31%, respectively. In addition, we found that the proportion of PC-3 cells sorted by MACS that expressed CD133 was significantly increased compared with that of the sphere-forming cells cultured in SFM (99.09 vs. 84.80%, P<0.05), while no difference was observed in the proportion of cells expressing CD44 between them (99.88 vs. 99.82%, P>0.05). The expression of PAP and AR as detected by western blot analysis of induced PCSLCs was significantly increased compared with that of uninduced PCSLCs (P<0.05); the proliferation capacity of PCSLCs was significantly higher than that of both the PC-3 cells (P<0.05) and induced PCSLCs (P<0.05). Furthermore, the PCSLCs that were isolated from SFM and MACS both demonstrated certain characteristics of stem cells and should be considered as stem cell-like. These data may hold potential for further exploring the role of PCSLCs.

语种: 英语
所属项目编号: 30972999
项目资助者: Natural Science Foundation of China (NSFC)
WOS记录号: WOS:000315423900011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50015
Appears in Collections:北京大学第一临床医学院_泌尿外科_期刊论文

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作者单位: 1.Chongqing Med Univ, Affiliated Hosp 1, Dept Urol, Chongqing 400016, Peoples R China
2.Chongqing Med Univ, Life Sci Res Inst, Chongqing 400016, Peoples R China
3.Peking Univ, Inst Urol, Beijing 100034, Peoples R China

Recommended Citation:
Sheng, Xia,Li, Zeng,Wang, De-Lin,et al. Isolation and enrichment of PC-3 prostate cancer stem-like cells using MACS and serum-free medium[J]. ONCOLOGY LETTERS,2013,5(3):787-792.
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