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Detecting specific cytotoxic T lymphocytes against SARS-coronavirus with DimerX HLA-A2 : Ig fusion protein
Wang, YD; Chen, WF
关键词SARS ELISPOT DimerX HLA-A2 immune response cytometry
刊名CLINICAL IMMUNOLOGY
2004-11-01
DOI10.1016/j.clim.2004.07.004
113期:2页:151-154
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Immunology
研究领域[WOS]Immunology
关键词[WOS]ACUTE RESPIRATORY SYNDROME ; IMMUNE-RESPONSE ; CELLS
英文摘要

To assess specific cytotoxic T lymphocytes (CTLs) against Severe acute respiratory syndrome (SARS)-coronavirus, a modified DimerX flow cytometry assay was performed with peripheral blood mononuclear cell (PBMC) from HLA-A2(+) SARS-recovered donors at different time points post disease. CD8(+) DimerX-S1203(+) CTLs were detected in the PBMC from these donors up to 3 months after recovery. The percentages of CD8(+) DimerX-S1203(+) cells paralleled the numbers of interferon-gamma-positive spots in an ELISPOT assay using the same antigenic peptide. In conclusion, DimerX-based flow cytometry staining may prove to be a real-time method to screen for CTL directed at epitopes from a newly identified virus. (C) 2004 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000224333400007
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被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
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条目标识符http://ir.bjmu.edu.cn/handle/400002259/50022
专题北京大学基础医学院_免疫学系
北京大学基础医学院
作者单位Peking Univ, Hlth Sci Ctr, Dept Immunol, Sch Basic Med Sci, Beijing 100083, Peoples R China
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Wang, YD,Chen, WF. Detecting specific cytotoxic T lymphocytes against SARS-coronavirus with DimerX HLA-A2 : Ig fusion protein[J]. CLINICAL IMMUNOLOGY,2004,113(2):151-154.
APA Wang, YD,&Chen, WF.(2004).Detecting specific cytotoxic T lymphocytes against SARS-coronavirus with DimerX HLA-A2 : Ig fusion protein.CLINICAL IMMUNOLOGY,113(2),151-154.
MLA Wang, YD,et al."Detecting specific cytotoxic T lymphocytes against SARS-coronavirus with DimerX HLA-A2 : Ig fusion protein".CLINICAL IMMUNOLOGY 113.2(2004):151-154.
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