北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 免疫学系  > 期刊论文
学科主题: 基础医学
题名:
Detecting specific cytotoxic T lymphocytes against SARS-coronavirus with DimerX HLA-A2 : Ig fusion protein
作者: Wang, YD; Chen, WF
关键词: SARS ; ELISPOT ; DimerX ; HLA-A2 ; immune response ; cytometry
刊名: CLINICAL IMMUNOLOGY
发表日期: 2004-11-01
DOI: 10.1016/j.clim.2004.07.004
卷: 113, 期:2, 页:151-154
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology
研究领域[WOS]: Immunology
关键词[WOS]: ACUTE RESPIRATORY SYNDROME ; IMMUNE-RESPONSE ; CELLS
英文摘要:

To assess specific cytotoxic T lymphocytes (CTLs) against Severe acute respiratory syndrome (SARS)-coronavirus, a modified DimerX flow cytometry assay was performed with peripheral blood mononuclear cell (PBMC) from HLA-A2(+) SARS-recovered donors at different time points post disease. CD8(+) DimerX-S1203(+) CTLs were detected in the PBMC from these donors up to 3 months after recovery. The percentages of CD8(+) DimerX-S1203(+) cells paralleled the numbers of interferon-gamma-positive spots in an ELISPOT assay using the same antigenic peptide. In conclusion, DimerX-based flow cytometry staining may prove to be a real-time method to screen for CTL directed at epitopes from a newly identified virus. (C) 2004 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000224333400007
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50022
Appears in Collections:基础医学院_免疫学系_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
1-s2.0-S1521661604002116-main.pdf(153KB)期刊论文出版稿限制开放 联系获取全文

作者单位: Peking Univ, Hlth Sci Ctr, Dept Immunol, Sch Basic Med Sci, Beijing 100083, Peoples R China

Recommended Citation:
Wang, YD,Chen, WF. Detecting specific cytotoxic T lymphocytes against SARS-coronavirus with DimerX HLA-A2 : Ig fusion protein[J]. CLINICAL IMMUNOLOGY,2004,113(2):151-154.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wang, YD]'s Articles
[Chen, WF]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wang, YD]‘s Articles
[Chen, WF]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace