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学科主题: 基础医学
题名:
Detecting specific cytotoxic T lymphocytes against SARS-coronavirus with DimerX HLA-A2 : Ig fusion protein
作者: Wang, YD; Chen, WF
关键词: SARS ; ELISPOT ; DimerX ; HLA-A2 ; immune response ; cytometry
刊名: CLINICAL IMMUNOLOGY
发表日期: 2004-11-01
DOI: 10.1016/j.clim.2004.07.004
卷: 113, 期:2, 页:151-154
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology
研究领域[WOS]: Immunology
关键词[WOS]: ACUTE RESPIRATORY SYNDROME ; IMMUNE-RESPONSE ; CELLS
英文摘要:

To assess specific cytotoxic T lymphocytes (CTLs) against Severe acute respiratory syndrome (SARS)-coronavirus, a modified DimerX flow cytometry assay was performed with peripheral blood mononuclear cell (PBMC) from HLA-A2(+) SARS-recovered donors at different time points post disease. CD8(+) DimerX-S1203(+) CTLs were detected in the PBMC from these donors up to 3 months after recovery. The percentages of CD8(+) DimerX-S1203(+) cells paralleled the numbers of interferon-gamma-positive spots in an ELISPOT assay using the same antigenic peptide. In conclusion, DimerX-based flow cytometry staining may prove to be a real-time method to screen for CTL directed at epitopes from a newly identified virus. (C) 2004 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000224333400007
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50022
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: Peking Univ, Hlth Sci Ctr, Dept Immunol, Sch Basic Med Sci, Beijing 100083, Peoples R China

Recommended Citation:
Wang, YD,Chen, WF. Detecting specific cytotoxic T lymphocytes against SARS-coronavirus with DimerX HLA-A2 : Ig fusion protein[J]. CLINICAL IMMUNOLOGY,2004,113(2):151-154.
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