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学科主题: 临床医学
题名:
Rosiglitazone inhibits migration, proliferation, and phenotypic differentiation in cultured human lung fibroblasts
作者: Lin, Qing; Fang, Li-Ping; Zhou, Wei-Wei; Liu, Xin-Min
关键词: human lung fibroblasts ; migration ; peroxisome proliferator-activated receptor gamma ; phenotypic differentiation ; proliferation ; rosiglitazone
刊名: EXPERIMENTAL LUNG RESEARCH
发表日期: 2010-03-01
DOI: 10.3109/01902140903214659
卷: 36, 期:2, 页:120-128
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Respiratory System
研究领域[WOS]: Respiratory System
关键词[WOS]: ACTIVATED RECEPTOR-GAMMA ; IDIOPATHIC PULMONARY-FIBROSIS ; 15-DEOXY-DELTA(12,14)-PROSTAGLANDIN J(2) ; INFLAMMATION ; MECHANISMS ; AGONISTS ; PPARS ; ALPHA ; CELLS
英文摘要:

Recent studies have indicated that peroxisome proliferator-activated receptor gamma (PPAR gamma) is capable of modulating inflammation, which prompted us to investigate the potential of PPAR gamma ligands as lung protective agents in pulmonary fibrosis. The present study was undertaken to investigate the effects of rosiglitazone (RSG), a highly potent ligand of PPAR gamma, on migration, proliferation, and phenotypic differentiation of human lung fibroblasts (MRC-5) and to explore its potential for therapy of pulmonary fibrosis. The cell migration potential was observed in a scratch wound model. Cell proliferation was determined by the MIT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) method, immunocytochemical staining, and flow cytometry, and protein expression by Western blot analysis. RSG slowed cell migration distance induced by fetal bovine serum (FBS), decreased cell proliferation initiated by FBS or platelet-derived growth factor-BB (PDGF-BB), and decreased a-smooth muscle actin (alpha-SMA) protein expression induced by transforming growth factor-beta 1 (TGF-beta 1). In addition, RSG incubation reduced the ratio of phospho-extracellular signal-regulated kinases 1/2 (p-ERK1/2) to ERK1/2 expression stimulated by FBS, PDGF-BB, and TGF-beta 1. These findings show that RSG treatment inhibits lung fibroblast migration and proliferation and myofibroblast transdifferentiation stimulated by FBS and growth factors in vitro, which suggests that PPAR gamma agonists could antagonize pulmonary fibrosis and have potential for therapeutic application in pulmonary fibrosis.

语种: 英语
WOS记录号: WOS:000276077100007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50030
Appears in Collections:北京大学第一临床医学院_老年病内科_期刊论文

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作者单位: Peking Univ, Hosp 1, Dept Geriatr, Beijing 100034, Peoples R China

Recommended Citation:
Lin, Qing,Fang, Li-Ping,Zhou, Wei-Wei,et al. Rosiglitazone inhibits migration, proliferation, and phenotypic differentiation in cultured human lung fibroblasts[J]. EXPERIMENTAL LUNG RESEARCH,2010,36(2):120-128.
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