北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 病原生物学系  > 期刊论文
学科主题: 基础医学
题名:
Distinct effects of knocking down MEK1 and MEK2 on replication of herpes simplex virus type 2
作者: Zhang, Hao1,2; Feng, Hai1; Luo, Lingqi1; Zhou, Qi1; Luo, Zhijun3; Peng, Yihong1,2
关键词: HSV-2 ; Viral replication ; MEK1 ; MEK2 ; ERK ; siRNA
刊名: VIRUS RESEARCH
发表日期: 2010-06-01
DOI: 10.1016/j.virusres.2010.02.007
卷: 150, 期:1-2, 页:22-27
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Virology
研究领域[WOS]: Virology
关键词[WOS]: ERK SIGNALING PATHWAY ; MAP KINASE ; RAS/RAF/MEK/ERK PATHWAY ; CELL-PROLIFERATION ; MAMMALIAN-CELLS ; INHIBITION ; ACTIVATION ; GROWTH ; INFECTION ; APOPTOSIS
英文摘要:

During infection, viruses hijack various host cell components and programs for their amplification, among which is the canonical ERK signaling pathway, mainly consisting of three tiered serine/threonine kinases, Rat, MEK and ERK. MEK1 and MEK2 are two isoforms of the kinase operating immediately upstream of ERK, and connecting Raf and ERK by phosphorylating ERR. Previous studies have suggested that different isoforms of MEK have distinct biological functions, although their in vitro kinase function may be redundant. However, little is known about the isoform-specific effects of these kinases on viral propagation. In this study, we showed that herpes simplex virus type 2 (HSV-2) infection of human embryonic kidney (HER) 293 cells induced a sustained activation of ERK1/2. Inhibition of this ERK activation by U0126, a specific inhibitor of MEK1/2, severely impaired virus production. A similar reduction of virus production was also seen following transfection of cells with siRNAs for MEK1/2. Interestingly, a specific knockdown of MEK1 with siRNAs caused a marked inhibition of viral titers, viral proteins and virus-induced cytopathic effect (CPE), whereas silencing MEK2 had little effect. Therefore, our results demonstrate that MEK1 and MEK2 act differently and that HSV-2 hijacks host MEK1 for its own amplification. To our knowledge, this is the first report showing inhibition of HSV-2 replication by targeting human MEK1. This study also suggests that MEK1 could be a potential target for anti-HSV-2 therapy, which may minimize damage to the host cells engendered by targeting both MEK1 and MEK2. (C) 2010 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 2007AA02Z317 ; 30470084
项目资助者: National High-Tech Research and Development Program of China (863 Program) ; National Natural Science Foundation of China
WOS记录号: WOS:000277821200003
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50049
Appears in Collections:基础医学院_病原生物学系_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
Distinct effects of knocking down MEK1 and MEK2 on replication of herpes simplex virus type 2.pdf(881KB)期刊论文出版稿限制开放 联系获取全文

作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100191, Peoples R China
2.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
3.Boston Univ, Sch Med, Dept Biochem, Boston, MA 02118 USA

Recommended Citation:
Zhang, Hao,Feng, Hai,Luo, Lingqi,et al. Distinct effects of knocking down MEK1 and MEK2 on replication of herpes simplex virus type 2[J]. VIRUS RESEARCH,2010,150(1-2):22-27.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhang, Hao]'s Articles
[Feng, Hai]'s Articles
[Luo, Lingqi]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhang, Hao]‘s Articles
[Feng, Hai]‘s Articles
[Luo, Lingqi]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace