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学科主题: 基础医学
题名:
Four-dimensional visualization and quantitative analysis of meiotic spindle movements in live mouse oocytes
作者: Tian, N.1; Zhang, L.2; Liu, B.1; Wang, P.1; Li, Y.2; Ma, W.1
关键词: Calcium ions ; live oocytes ; real-time ; spindle ; two-photon
刊名: JOURNAL OF MICROSCOPY
发表日期: 2012-09-01
DOI: 10.1111/j.1365-2818.2012.03640.x
卷: 247, 期:3, 页:252-258
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Microscopy
研究领域[WOS]: Microscopy
关键词[WOS]: CALCIUM-IONS ; POLAR BODY ; IN-VITRO ; METAPHASE ; ROTATION ; DIVISION ; MICROTUBULES ; ORGANIZATION ; MATURATION ; DYNAMICS
英文摘要:

This paper made a different attempt of real-time observation of the meiotic spindle movements in living mouse oocyte using a convenient method. This method was based on an experimental phenomenon discovered in our work. In living mouse oocytes, a high concentration of calcium ions (Ca2+) was observed throughout the region occupied by the initial meiotic spindle. After Ca2+ labelling with Fura-2, a weakly fluorescent area (WFA) appeared on each side of the chromosomes. The activities of the WFAs changed during spindle development. By real-time tracking of WFAs, we were able to indirectly observe the meiotic spindle movements. Occasionally, it was observed that the first meiotic spindle rotated from an orientation parallel to the cortex to become perpendicular, instead of migrating from the oocyte centre to the cortex along its axis. Moreover, we analysed this uncommon rotation of the first meiotic spindle and found that the whole rotation process can be divided into two phases: the early slow-speed rotation and the subsequent rapid-speed rotation. We further characterized this rotation with respect to rotational speed and acceleration at all the stages of development. By using a two-photon laser-scanning microscope in combination with Fura-2 dye that is nondamaging to oocytes, we provide a convenient method for indirect visualization and quantitative analysis of spindle movements by real-time tracking of WFAs. This method is easy to operate and master, and economical with time and effort.

语种: 英语
所属项目编号: 90919012 ; 10874099 ; 2007CB5119004 ; 20090002110065 ; 2010THZ01
项目资助者: National Nature Science Foundation of China ; Chinese National Basic Research Program ; Doctoral Program Research Fund of Chinese Ministry of Education ; Tsinghua University Initiative Scientific Research Program
WOS记录号: WOS:000307968600005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50050
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Tsinghua Univ, Dept Phys, State Key Lab Low Dimens Quantum Phys, Beijing 100084, Peoples R China
2.Peking Univ, Dept Histol & Embryol, Sch Basic Med Sci, Hlth Sci Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Tian, N.,Zhang, L.,Liu, B.,et al. Four-dimensional visualization and quantitative analysis of meiotic spindle movements in live mouse oocytes[J]. JOURNAL OF MICROSCOPY,2012,247(3):252-258.
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