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学科主题基础医学
Four-dimensional visualization and quantitative analysis of meiotic spindle movements in live mouse oocytes
Tian, N.1; Zhang, L.2; Liu, B.1; Wang, P.1; Li, Y.2; Ma, W.1
关键词Calcium Ions Live Oocytes Real-time Spindle Two-photon
刊名JOURNAL OF MICROSCOPY
2012-09-01
DOI10.1111/j.1365-2818.2012.03640.x
247期:3页:252-258
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Microscopy
研究领域[WOS]Microscopy
关键词[WOS]CALCIUM-IONS ; POLAR BODY ; IN-VITRO ; METAPHASE ; ROTATION ; DIVISION ; MICROTUBULES ; ORGANIZATION ; MATURATION ; DYNAMICS
英文摘要

This paper made a different attempt of real-time observation of the meiotic spindle movements in living mouse oocyte using a convenient method. This method was based on an experimental phenomenon discovered in our work. In living mouse oocytes, a high concentration of calcium ions (Ca2+) was observed throughout the region occupied by the initial meiotic spindle. After Ca2+ labelling with Fura-2, a weakly fluorescent area (WFA) appeared on each side of the chromosomes. The activities of the WFAs changed during spindle development. By real-time tracking of WFAs, we were able to indirectly observe the meiotic spindle movements. Occasionally, it was observed that the first meiotic spindle rotated from an orientation parallel to the cortex to become perpendicular, instead of migrating from the oocyte centre to the cortex along its axis. Moreover, we analysed this uncommon rotation of the first meiotic spindle and found that the whole rotation process can be divided into two phases: the early slow-speed rotation and the subsequent rapid-speed rotation. We further characterized this rotation with respect to rotational speed and acceleration at all the stages of development. By using a two-photon laser-scanning microscope in combination with Fura-2 dye that is nondamaging to oocytes, we provide a convenient method for indirect visualization and quantitative analysis of spindle movements by real-time tracking of WFAs. This method is easy to operate and master, and economical with time and effort.

语种英语
WOS记录号WOS:000307968600005
项目编号90919012 ; 10874099 ; 2007CB5119004 ; 20090002110065 ; 2010THZ01
资助机构National Nature Science Foundation of China ; Chinese National Basic Research Program ; Doctoral Program Research Fund of Chinese Ministry of Education ; Tsinghua University Initiative Scientific Research Program
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50050
专题北京大学基础医学院
作者单位1.Tsinghua Univ, Dept Phys, State Key Lab Low Dimens Quantum Phys, Beijing 100084, Peoples R China
2.Peking Univ, Dept Histol & Embryol, Sch Basic Med Sci, Hlth Sci Ctr, Beijing 100191, Peoples R China
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GB/T 7714
Tian, N.,Zhang, L.,Liu, B.,et al. Four-dimensional visualization and quantitative analysis of meiotic spindle movements in live mouse oocytes[J]. JOURNAL OF MICROSCOPY,2012,247(3):252-258.
APA Tian, N.,Zhang, L.,Liu, B.,Wang, P.,Li, Y.,&Ma, W..(2012).Four-dimensional visualization and quantitative analysis of meiotic spindle movements in live mouse oocytes.JOURNAL OF MICROSCOPY,247(3),252-258.
MLA Tian, N.,et al."Four-dimensional visualization and quantitative analysis of meiotic spindle movements in live mouse oocytes".JOURNAL OF MICROSCOPY 247.3(2012):252-258.
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