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学科主题基础医学
The Formation and Stabilization of a Novel G-Quadruplex in the 5 ′-Flanking Region of the Relaxin Gene
Lin, Sen1; Gu, Huiping2; Xu, Ming2; Cui, Xiaojie1; Zhang, Youyi2; Gao, Wei2; Yuan, Gu1
刊名PLOS ONE
2012-02-21
DOI10.1371/journal.pone.0031201
7期:2
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]TELOMERIC G-QUADRUPLEX ; RELEVANT G-QUADRUPLEX ; C-MYC PROMOTER ; BERBERINE DERIVATIVES ; AUTOMATED DOCKING ; NUCLEIC-ACIDS ; CIRCULAR-DICHROISM ; DRUG DESIGN ; DNA ; BINDING
英文摘要

It has been reported that binding of STAT3 protein to the 5′-flanking region of the relaxin gene may result in downregulation of the relaxin expression. There is a Guanine(G)-rich segment located in about 3.8 Kb upstream of the relaxin gene and very close to the STAT3′s binding site. In our study, NMR spectroscopy revealed the formation of G-quadruplex by this G-rich strand, and the result was confirmed by ESI mass spectrometry and CD spectroscopy. The theoretical structure of RLX G-quadruplex was constructed and refined by molecular modeling. When this relaxin G-quadruplex was stabilized by berberine(Delta Tm=10 degrees C), a natural alkaloid from a Chinese herb, the gene expression could be up-regulated in a dose-dependent manner which was proved by luciferase assay. This result is different from the general G-quadruplex function that inhibiting the telomere replication or down-regulating many oncogenes expression. Therefore, our study reported a novel G- quadruplex in the relaxin gene and complemented the regulation mechanism about gene expression by G- quadruplexes.

语种英语
WOS记录号WOS:000302873700031
项目编号90913004 ; 81070196
资助机构National Natural Science Foundation of China ; Program for New Century Excellent Talents in University ; Beijing Talents Foundation
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50051
专题北京大学基础医学院_心血管所
北京大学第三临床医学院_心血管内科
作者单位1.Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Dept Chem Biol,Coll Chem & Mol Engn, Beijing 100871, Peoples R China
2.Peking Univ, Key Lab Mol Cardiovasc Sci, Key Lab Cardiovasc Mol Biol & Regulatory Peptides, Minist Hlth,Hosp 3,Minist Educ,Inst Vasc Med, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Lin, Sen,Gu, Huiping,Xu, Ming,et al. The Formation and Stabilization of a Novel G-Quadruplex in the 5 ′-Flanking Region of the Relaxin Gene[J]. PLOS ONE,2012,7(2).
APA Lin, Sen.,Gu, Huiping.,Xu, Ming.,Cui, Xiaojie.,Zhang, Youyi.,...&Yuan, Gu.(2012).The Formation and Stabilization of a Novel G-Quadruplex in the 5 ′-Flanking Region of the Relaxin Gene.PLOS ONE,7(2).
MLA Lin, Sen,et al."The Formation and Stabilization of a Novel G-Quadruplex in the 5 ′-Flanking Region of the Relaxin Gene".PLOS ONE 7.2(2012).
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