IR@PKUHSC  > 北京大学基础医学院  > 心血管所
学科主题基础医学
The Formation and Stabilization of a Novel G-Quadruplex in the 5 ′-Flanking Region of the Relaxin Gene
Lin, Sen1; Gu, Huiping2; Xu, Ming2; Cui, Xiaojie1; Zhang, Youyi2; Gao, Wei2; Yuan, Gu1
刊名PLOS ONE
2012-02-21
DOI10.1371/journal.pone.0031201
7期:2
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
资助者National Natural Science Foundation of China ; Program for New Century Excellent Talents in University ; Beijing Talents Foundation ; National Natural Science Foundation of China ; Program for New Century Excellent Talents in University ; Beijing Talents Foundation
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]TELOMERIC G-QUADRUPLEX ; RELEVANT G-QUADRUPLEX ; C-MYC PROMOTER ; BERBERINE DERIVATIVES ; AUTOMATED DOCKING ; NUCLEIC-ACIDS ; CIRCULAR-DICHROISM ; DRUG DESIGN ; DNA ; BINDING
英文摘要

It has been reported that binding of STAT3 protein to the 5′-flanking region of the relaxin gene may result in downregulation of the relaxin expression. There is a Guanine(G)-rich segment located in about 3.8 Kb upstream of the relaxin gene and very close to the STAT3′s binding site. In our study, NMR spectroscopy revealed the formation of G-quadruplex by this G-rich strand, and the result was confirmed by ESI mass spectrometry and CD spectroscopy. The theoretical structure of RLX G-quadruplex was constructed and refined by molecular modeling. When this relaxin G-quadruplex was stabilized by berberine(Delta Tm=10 degrees C), a natural alkaloid from a Chinese herb, the gene expression could be up-regulated in a dose-dependent manner which was proved by luciferase assay. This result is different from the general G-quadruplex function that inhibiting the telomere replication or down-regulating many oncogenes expression. Therefore, our study reported a novel G- quadruplex in the relaxin gene and complemented the regulation mechanism about gene expression by G- quadruplexes.

语种英语
所属项目编号90913004 ; 81070196
资助者National Natural Science Foundation of China ; Program for New Century Excellent Talents in University ; Beijing Talents Foundation ; National Natural Science Foundation of China ; Program for New Century Excellent Talents in University ; Beijing Talents Foundation
WOS记录号WOS:000302873700031
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50051
Collection北京大学基础医学院_心血管所
作者单位1.Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Dept Chem Biol,Coll Chem & Mol Engn, Beijing 100871, Peoples R China
2.Peking Univ, Key Lab Mol Cardiovasc Sci, Key Lab Cardiovasc Mol Biol & Regulatory Peptides, Minist Hlth,Hosp 3,Minist Educ,Inst Vasc Med, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Lin, Sen,Gu, Huiping,Xu, Ming,et al. The Formation and Stabilization of a Novel G-Quadruplex in the 5 ′-Flanking Region of the Relaxin Gene[J]. PLOS ONE,2012,7(2).
APA Lin, Sen.,Gu, Huiping.,Xu, Ming.,Cui, Xiaojie.,Zhang, Youyi.,...&Yuan, Gu.(2012).The Formation and Stabilization of a Novel G-Quadruplex in the 5 ′-Flanking Region of the Relaxin Gene.PLOS ONE,7(2).
MLA Lin, Sen,et al."The Formation and Stabilization of a Novel G-Quadruplex in the 5 ′-Flanking Region of the Relaxin Gene".PLOS ONE 7.2(2012).
Files in This Item: Download All
File Name/Size DocType Version Access License
journal.pone.0031201(380KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
谷歌学术
谷歌学术Similar articles in
[Lin, Sen]'s Articles
[Gu, Huiping]'s Articles
[Xu, Ming]'s Articles
百度学术
百度学术Similar articles in
[Lin, Sen]'s Articles
[Gu, Huiping]'s Articles
[Xu, Ming]'s Articles
必应学术
必应学术Similar articles in
[Lin, Sen]'s Articles
[Gu, Huiping]'s Articles
[Xu, Ming]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: journal.pone.0031201.PDF
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.