北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 感染疾病科  > 期刊论文
学科主题: 临床医学
题名:
Molecular and Microscopic Analysis of Bacteria and Viruses in Exhaled Breath Collected Using a Simple Impaction and Condensing Method
作者: Shen, Fangxia1; Xu, Zhenqiang1; Li, Xiaoguang2; Wu, Yan1; Chen, Qi1; Jie, Xu2; Yao, Maosheng1
刊名: PLOS ONE
发表日期: 2012-07-25
DOI: 10.1371/journal.pone.0041137
卷: 7, 期:7
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: CELL LUNG-CANCER ; INFLUENZA ; AIR ; TRANSMISSION ; INFECTIONS ; BIOMARKER ; OUTBREAK ; AEROSOLS ; EXPOSURE ; CHILDREN
英文摘要:

Exhaled breath condensate (EBC) is increasingly being used as a non-invasive method for disease diagnosis and environmental exposure assessment. By using hydrophobic surface, ice, and droplet scavenging, a simple impaction and condensing based collection method is reported here. Human subjects were recruited to exhale toward the device for 1, 2, 3, and 4 min. The exhaled breath quickly formed into tiny droplets on the hydrophobic surface, which were subsequently scavenged into a 10 mu L rolling deionized water droplet. The collected EBC was further analyzed using culturing, DNA stain, Scanning Electron Microscope (SEM), polymerase chain reaction (PCR) and colorimetry (VITEK 2) for bacteria and viruses. Experimental data revealed that bacteria and viruses in EBC can be rapidly collected using the method developed here, with an observed efficiency of 100 mu L EBC within 1 min. Culturing, DNA stain, SEM, and qPCR methods all detected high bacterial concentrations up to 7000 CFU/m(3) in exhaled breath, including both viable and dead cells of various types. Sphingomonas paucimobilis and Kocuria variants were found dominant in EBC samples using VITEK 2 system. SEM images revealed that most bacteria in exhaled breath are detected in the size range of 0.5-1.0 mu m, which is able to enable them to remain airborne for a longer time, thus presenting a risk for airborne transmission of potential diseases. Using qPCR, influenza A H3N2 viruses were also detected in one EBC sample. Different from other devices restricted solely to condensation, the developed method can be easily achieved both by impaction and condensation in a laboratory and could impact current practice of EBC collection. Nonetheless, the reported work is a proof-of-concept demonstration, and its performance in non-invasive disease diagnosis such as bacterimia and virus infections needs to be further validated including effects of its influencing matrix.

语种: 英语
所属项目编号: 21077005 ; 20877004 ; 2008AA062503
项目资助者: National Science Foundation of China ; National High Technology Research and Development Program of China
WOS记录号: WOS:000306806600041
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50080
Appears in Collections:北京大学第三临床医学院_感染疾病科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, State Key Joint Lab Environm Simulat & Pollut Con, Coll Environm Sci & Engn, Beijing 100871, Peoples R China
2.Peking Univ, Hosp 3, Dept Infect Dis, Beijing 100871, Peoples R China

Recommended Citation:
Shen, Fangxia,Xu, Zhenqiang,Li, Xiaoguang,et al. Molecular and Microscopic Analysis of Bacteria and Viruses in Exhaled Breath Collected Using a Simple Impaction and Condensing Method[J]. PLOS ONE,2012,7(7).
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Shen, Fangxia]'s Articles
[Xu, Zhenqiang]'s Articles
[Li, Xiaoguang]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Shen, Fangxia]‘s Articles
[Xu, Zhenqiang]‘s Articles
[Li, Xiaoguang]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace