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学科主题临床医学
Molecular and Microscopic Analysis of Bacteria and Viruses in Exhaled Breath Collected Using a Simple Impaction and Condensing Method
Shen, Fangxia1; Xu, Zhenqiang1; Li, Xiaoguang2; Wu, Yan1; Chen, Qi1; Jie, Xu2; Yao, Maosheng1
刊名PLOS ONE
2012-07-25
DOI10.1371/journal.pone.0041137
7期:7
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]CELL LUNG-CANCER ; INFLUENZA ; AIR ; TRANSMISSION ; INFECTIONS ; BIOMARKER ; OUTBREAK ; AEROSOLS ; EXPOSURE ; CHILDREN
英文摘要

Exhaled breath condensate (EBC) is increasingly being used as a non-invasive method for disease diagnosis and environmental exposure assessment. By using hydrophobic surface, ice, and droplet scavenging, a simple impaction and condensing based collection method is reported here. Human subjects were recruited to exhale toward the device for 1, 2, 3, and 4 min. The exhaled breath quickly formed into tiny droplets on the hydrophobic surface, which were subsequently scavenged into a 10 mu L rolling deionized water droplet. The collected EBC was further analyzed using culturing, DNA stain, Scanning Electron Microscope (SEM), polymerase chain reaction (PCR) and colorimetry (VITEK 2) for bacteria and viruses. Experimental data revealed that bacteria and viruses in EBC can be rapidly collected using the method developed here, with an observed efficiency of 100 mu L EBC within 1 min. Culturing, DNA stain, SEM, and qPCR methods all detected high bacterial concentrations up to 7000 CFU/m(3) in exhaled breath, including both viable and dead cells of various types. Sphingomonas paucimobilis and Kocuria variants were found dominant in EBC samples using VITEK 2 system. SEM images revealed that most bacteria in exhaled breath are detected in the size range of 0.5-1.0 mu m, which is able to enable them to remain airborne for a longer time, thus presenting a risk for airborne transmission of potential diseases. Using qPCR, influenza A H3N2 viruses were also detected in one EBC sample. Different from other devices restricted solely to condensation, the developed method can be easily achieved both by impaction and condensation in a laboratory and could impact current practice of EBC collection. Nonetheless, the reported work is a proof-of-concept demonstration, and its performance in non-invasive disease diagnosis such as bacterimia and virus infections needs to be further validated including effects of its influencing matrix.

语种英语
WOS记录号WOS:000306806600041
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50080
专题北京大学第三临床医学院_感染疾病科
作者单位1.Peking Univ, State Key Joint Lab Environm Simulat & Pollut Con, Coll Environm Sci & Engn, Beijing 100871, Peoples R China
2.Peking Univ, Hosp 3, Dept Infect Dis, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Shen, Fangxia,Xu, Zhenqiang,Li, Xiaoguang,et al. Molecular and Microscopic Analysis of Bacteria and Viruses in Exhaled Breath Collected Using a Simple Impaction and Condensing Method[J]. PLOS ONE,2012,7(7).
APA Shen, Fangxia.,Xu, Zhenqiang.,Li, Xiaoguang.,Wu, Yan.,Chen, Qi.,...&Yao, Maosheng.(2012).Molecular and Microscopic Analysis of Bacteria and Viruses in Exhaled Breath Collected Using a Simple Impaction and Condensing Method.PLOS ONE,7(7).
MLA Shen, Fangxia,et al."Molecular and Microscopic Analysis of Bacteria and Viruses in Exhaled Breath Collected Using a Simple Impaction and Condensing Method".PLOS ONE 7.7(2012).
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