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学科主题: 临床医学
题名:
PTEN expression and suppression of proliferation are associated with Cdx2 overexpression in gastric cancer cells
作者: Bai, Zhi-Gang1; Ye, Ying-Jiang2; Shen, Dan-Hua3; Lu, You-Yong4; Zhang, Zhong-Tao1; Wang, Shan2
关键词: gastric cancer ; Cdx2 ; PTEN ; PI3K
刊名: INTERNATIONAL JOURNAL OF ONCOLOGY
发表日期: 2013-05-01
DOI: 10.3892/ijo.2013.1875
卷: 42, 期:5, 页:1682-1691
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: INTESTINAL METAPLASIA ; NUCLEAR PTEN ; PROGNOSIS ; CARCINOMA ; CARCINOGENESIS ; STOMACH ; PATHWAY ; MICE
英文摘要:

The prognosis of gastric cancer (GC) is associated with Cdx2 and nuclear PTEN coexpression. This study aimed to determine the expression patterns of Cdx2 and PTEN in various GC tissues and cell lines to identify their relationship in GC. Immunohistochemistry was undertaken to assess the expression patterns of Cdx2 and PTEN in paraffin-embedded specimens of 228 GC patients who had undergone radical D2 gastrostomy with long-term follow-up. Cell growth and tumorigenicity were analyzed in the BGC823 cells with exogenous Cdx2 and any changes in the associated signaling pathways were interpreted in exogenous cdx2 expression and cdx2 knockdown. Cdx2 was found in the nuclei of GC cells in 43.4% (99/228) of the paraffin-embedded biopsies. A higher expression of nuclear PTEN was observed in 36.4% (83/228). Coexpression of Cdx2 and nuclear PTEN was detected in GC tumors (59/228, 25.9%) which correlated with the prognosis of advanced GC patients (p<0.001). The expression levels of Cdx2 and PTEN were variable in the different GC cell lines. However, the trends were similar between PTEN and Cdx2 in GC tissues and cell lines. High expression of Cdx2 and PTEN significantly reduced tumorigenicity in BGC823 cells compared with the empty vector control. Exogenous expression of Cdx2 triggered the upregulation of PTEN expression and decreased PI3K and pAkt expression and vice versa. The coexpression levels of PTEN and Cdx2 in GC tumors correlated with prognosis in GC patients. Cdx2 may play a role in the upregulation of PTEN by triggering PI3K/Akt inactivation in GC cells.

语种: 英语
所属项目编号: 2006AA02A402 ; 7112034 ; 7122052
项目资助者: National Bio-Tech 86-3 ; Beijing Natural Science Foundation
WOS记录号: WOS:000318053400021
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50081
Appears in Collections:北京大学第二临床医学院_胃肠外科_期刊论文

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作者单位: 1.Capital Med Univ, Beijing Friendship Hosp, Dept Gen Surg, Beijing, Peoples R China
2.Peking Univ, Peoples Hosp, Surg Oncol Lab, Dept Surg Gastroenterol, Beijing 100044, Peoples R China
3.Peking Univ, Peoples Hosp, Dept Pathol, Beijing 100044, Peoples R China
4.Peking Univ, Mol Oncol Lab, Key Lab Carcinogenesis & Translat Res, Minist Educ,Sch Oncol,Beijing Canc Hosp Inst, Beijing 100142, Peoples R China

Recommended Citation:
Bai, Zhi-Gang,Ye, Ying-Jiang,Shen, Dan-Hua,et al. PTEN expression and suppression of proliferation are associated with Cdx2 overexpression in gastric cancer cells[J]. INTERNATIONAL JOURNAL OF ONCOLOGY,2013,42(5):1682-1691.
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