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Investigation on the Binding of Terazosin Hydrochloride and Naftopidil to an Immobilized alpha (1)-Adrenoceptor by Zonal Elution
Zhang, Youyi3,4; Gao, Xiaokang1; Li, Qian1; Zhao, Xinfeng1,2; Huang, Jingjing1; Bian, Liujiao1; Zheng, Jianbin2; Li, Zijian3,4; Zheng, Xiaohui1,2
关键词Alpha(1)-adrenoceptor High-performance Affinity Chromatography Naftopidil Terazosin Hydrochloride
刊名CHROMATOGRAPHIA
2014-09-01
DOI10.1007/s10337-014-2716-7
77期:17-18页:1235-1239
收录类别SCI ; ISTP
文章类型Proceedings Paper
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]PERFORMANCE AFFINITY-CHROMATOGRAPHY ; BENIGN PROSTATIC HYPERPLASIA ; HUMAN SERUM-ALBUMIN ; CARBAMAZEPINE BINDING ; INTERNATIONAL UNION ; NOMENCLATURE ; PHARMACOLOGY
英文摘要

The interaction between drugs and receptors is particularly important in revealing the drug acting mechanism and developing new leads. In this work, alpha (1)-Adrenoceptor (alpha (1)-AR) from HEK293 cell line is purified and immobilized on the surface of macro-pore silica gel to prepare an high-performance affinity chromatography stationary phase for the pursuit of drug-receptor interactions by competition zonal elution. Naftopidil is found to have only one type of binding site to alpha (1)-AR with an association constant of 1.45 x 10(6) M-1 and a concentration of binding sites of 1.56 x 10(-6) M, while terazosin hydrochloride proves to present two kinds of binding site on the receptor at which the association constants are determined to be 1.61 x 10(5) M-1 and 2.06 x 10(3) M-1, and the corresponding concentrations of the binding sites are 1.56 x 10(-6) M and 1.11 x 10(-3) M, respectively. It is concluded that the stationary phase containing attached alpha (1)-AR can be used to realize the binding of a drug to the receptor.

语种英语
WOS记录号WOS:000341659000014
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50084
专题北京大学第三临床医学院_心血管内科
作者单位1.NW Univ Xian, Key Lab Resource Biol & Biotechnol Western China, Minist Educ, Xian 710069, Peoples R China
2.NW Univ Xian, Inst Analyt Sci, Xian 710069, Shaanxi, Peoples R China
3.Peking Univ, Inst Vasc Med, Hosp 3, Beijing 100083, Peoples R China
4.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100083, Peoples R China
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GB/T 7714
Zhang, Youyi,Gao, Xiaokang,Li, Qian,et al. Investigation on the Binding of Terazosin Hydrochloride and Naftopidil to an Immobilized alpha (1)-Adrenoceptor by Zonal Elution[J]. CHROMATOGRAPHIA,2014,77(17-18):1235-1239.
APA Zhang, Youyi.,Gao, Xiaokang.,Li, Qian.,Zhao, Xinfeng.,Huang, Jingjing.,...&Zheng, Xiaohui.(2014).Investigation on the Binding of Terazosin Hydrochloride and Naftopidil to an Immobilized alpha (1)-Adrenoceptor by Zonal Elution.CHROMATOGRAPHIA,77(17-18),1235-1239.
MLA Zhang, Youyi,et al."Investigation on the Binding of Terazosin Hydrochloride and Naftopidil to an Immobilized alpha (1)-Adrenoceptor by Zonal Elution".CHROMATOGRAPHIA 77.17-18(2014):1235-1239.
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