|Role of phosphatase PTEN in the activation of extracellular signal-regulated kinases induced by estradiol in endometrial carcinoma cells|
|Zhang, YJ1; Wei, LH1; Wang, JL1; Sun, TZ1|
|关键词||Endometrial Carcinoma Phosphatase And Tensin Homologue Estradiol Extracellular Signal-regulated Kinase|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
|关键词[WOS]||RECEPTOR ; PATHWAY|
Objectives To study extracellular signal-regulated kinase (ERK) activation in the endometrial carcinoma cell line lshikawa with stimulation by 17-beta-estradiol, and to elucidate the role of phosphatase and tensin homologue (PTEN) and estrogen receptor (ER) subtype on the activation of ERKs.
Methods Western blot was used to examine the expression of PTEN and PTEN (G129E) in Ishikawa cells after stable transfection as well as ERK activation in lshikawa-EGFP, lshikawa- PTEN and Ishikawa-PTEN (G129E) stimulated with various doses of 17-beta-estradiol for different lengths of time. Western blot was also used for examining the expression of ERalpha and ERbeta in NlH3T3 fibroblasts after transient transfection of pCXN2hERalpha and pCXN2hERbeta. Then, ERK activation was examined after stimulation with 17-beta-estradiol.
Results 17-beta-estradiol activated ERK cascades (mainly ERK2) in Ishikawa cells. The activation of ERK increased gradually as concentration of 17-beta-estradiol also increased. The maximal activation of ERK2 took place 5 min after stimulation with 17-beta-estradiol. The activation of ERK2 was inhibited markedly by PTEN, but not by PTEN (G129E). 17-beta-estradiol activated ERK cascades in NlH3T3 fibroblasts after transient transfection of pCXN2hERa.
Conclusions 17-beta-estradiol activate ERK cascades in Ishikawa cells by integrating with ERa. Lipid phosphatase PTEN has an inhibitory role on the activation of ERK stimulated by 17-beta-estradiol in Ishikawa cells.
|作者单位||1.Peking Univ, Peoples Hosp, Dept Gynecol, Beijing 100044, Peoples R China|
2.Peking Univ, Peoples Hosp, Arthrit Inst, Beijing 100044, Peoples R China
|Zhang, YJ,Wei, LH,Wang, JL,et al. Role of phosphatase PTEN in the activation of extracellular signal-regulated kinases induced by estradiol in endometrial carcinoma cells[J]. CHINESE MEDICAL JOURNAL,2003,116(3):383-387.|
|APA||Zhang, YJ,Wei, LH,Wang, JL,&Sun, TZ.(2003).Role of phosphatase PTEN in the activation of extracellular signal-regulated kinases induced by estradiol in endometrial carcinoma cells.CHINESE MEDICAL JOURNAL,116(3),383-387.|
|MLA||Zhang, YJ,et al."Role of phosphatase PTEN in the activation of extracellular signal-regulated kinases induced by estradiol in endometrial carcinoma cells".CHINESE MEDICAL JOURNAL 116.3(2003):383-387.|