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学科主题: 临床医学
题名:
Validation of a fast and reliable liquid chromatography-tandem mass spectrometry (LC-MS/MS) with atmospheric pressure chemical ionization method for simultaneous quantitation of voriconazole, itraconazole and its active metabolite hydroxyitraconazole in human plasma
作者: Xiong, Xin1,2; Zhai, Suodi1; Duan, Jingli1
关键词: human plasma ; hydroxyitraconazole ; itraconazole ; LC-APCI-MS/MS ; voriconazole
刊名: CLINICAL CHEMISTRY AND LABORATORY MEDICINE
发表日期: 2013-02-01
DOI: 10.1515/cclm-2012-0364
卷: 51, 期:2, 页:339-346
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medical Laboratory Technology
研究领域[WOS]: Medical Laboratory Technology
关键词[WOS]: SIMULTANEOUS QUANTIFICATION ; FUNGAL-INFECTIONS ; BIOEQUIVALENCE ; ELECTROSPRAY ; POSACONAZOLE ; FLUCONAZOLE ; MANAGEMENT
英文摘要:

Background: Voriconazole and itraconazole are two broad-spectrum antifungal triazole derivates administered for the prevention and in the treatment of invasive fungal infections. Their broad inter-and intra-individual pharmacokinetic variability and the high probability of drug-drug interactions justify therapeutic drug monitoring.

Methods: After liquid-liquid extraction with tertbutyl methyl ether, chromatographic separation was achieved on a Zorbax Eclipse XDB-C18 column using gradient elution with 10 mM ammonium formate and acetonitrile. Detection was performed by a tandem mass spectrometer coupled to LC via an atmospheric pressure chemical ionization (APCI) and quantification was performed using selected reaction monitoring (SRM) transitions

Results: Total run time was 4.5 min. The method was validated for concentrations ranging from 0.05 to 10 mu g/mL for voriconazole and from 0.025 to 5 mu g/mL for itraconazole and hydroxyitraconazole, respectively. The intra-and inter-day correlation coefficients of variation were <7.7%-<9.2%, respectively. The accuracy ranged from 92.6% to 109%.

Conclusions: A rapid and simple liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry (LC-APCI-MS/MS) method has been developed and validated to measure voriconazole itraconazole and hydroxyitraconazole in human plasma. This method is successfully applied to samples from patients receiving antifungal treatment.

语种: 英语
WOS记录号: WOS:000314999000024
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50101
Appears in Collections:北京大学第二临床医学院_药剂科_期刊论文

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作者单位: 1.Peking Univ, Dept Pharm, Hosp 2, Beijing 100191, Peoples R China
2.Peking Univ, Therapeut Drug Monitoring & Clin Toxicol Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Xiong, Xin,Zhai, Suodi,Duan, Jingli. Validation of a fast and reliable liquid chromatography-tandem mass spectrometry (LC-MS/MS) with atmospheric pressure chemical ionization method for simultaneous quantitation of voriconazole, itraconazole and its active metabolite hydroxyitraconazole in human plasma[J]. CLINICAL CHEMISTRY AND LABORATORY MEDICINE,2013,51(2):339-346.
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