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学科主题: 临床医学
题名:
Involvement of the cGMP signalling pathway in the regulation of viability in insulin-secreting BRIN-BD11 cells
作者: Kaminski, A1; Gao, HW1; Morgan, NG1
关键词: diabetes ; apoptosis ; cGMP-dependent kinase ; islets of Langerhans ; YC-1 ; guanylyl cyclase
刊名: FEBS LETTERS
发表日期: 2004-02-13
DOI: 10.1016/S0014-5793(04)00048-1
卷: 559, 期:1-3, 页:118-124
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
关键词[WOS]: PANCREATIC BETA-CELLS ; SOLUBLE GUANYLYL CYCLASE ; RAT CARDIAC MYOCYTES ; NITRIC-OXIDE ; INDUCED APOPTOSIS ; NATRIURETIC-PEPTIDE ; DNA-DAMAGE ; CYCLIC-GMP ; ISLETS ; LANGERHANS
英文摘要:

We have evaluated the hypothesis that cGMP may serve as an intracellular messenger regulating the viability of pancreatic beta-cells. A direct activator of soluble guanylyl cyclase, YC-1, caused a time- and dose-dependent loss of viability in clonal BRIN-BD11 beta-cells. This was accompanied by a rise in cGMP and was antagonised by Rp-8-pCPT-cGMPS, a selective inhibitor of protein kinase G (PKG). Reverse transcription polymerase chain reaction analysis confirmed that BRIN-BD11 cells (and human islets) express all three known isoforms of PKG (PKG-Ialpha, -Ibeta and II). Cell death induced by YC-1 was not sensitive to cell-permeable caspase inhibitors and was not accompanied by oligonucleosomal DNA fragmentation. The response was, however, inhibited by actinomycin D, suggesting that a transcription-dependent pathway of programmed cell death is involved in the actions of cGMP. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000188970100021
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50109
Appears in Collections:北京大学第三临床医学院_内分泌科_期刊论文

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作者单位: 1.Peninsula Med Sch, Inst Biomed & Clin Sci, Endocrine Pharmacol Grp, Plymouth PL6 8BX, Devon, England
2.Peking Univ, Hosp 3, Dept Endocrinol, Beijing 100871, Peoples R China

Recommended Citation:
Kaminski, A,Gao, HW,Morgan, NG. Involvement of the cGMP signalling pathway in the regulation of viability in insulin-secreting BRIN-BD11 cells[J]. FEBS LETTERS,2004,559(1-3):118-124.
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