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Involvement of the cGMP signalling pathway in the regulation of viability in insulin-secreting BRIN-BD11 cells
Kaminski, A1; Gao, HW1; Morgan, NG1
关键词Diabetes Apoptosis Cgmp-dependent Kinase Islets Of Langerhans Yc-1 Guanylyl Cyclase
刊名FEBS LETTERS
2004-02-13
DOI10.1016/S0014-5793(04)00048-1
559期:1-3页:118-124
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
关键词[WOS]PANCREATIC BETA-CELLS ; SOLUBLE GUANYLYL CYCLASE ; RAT CARDIAC MYOCYTES ; NITRIC-OXIDE ; INDUCED APOPTOSIS ; NATRIURETIC-PEPTIDE ; DNA-DAMAGE ; CYCLIC-GMP ; ISLETS ; LANGERHANS
英文摘要

We have evaluated the hypothesis that cGMP may serve as an intracellular messenger regulating the viability of pancreatic beta-cells. A direct activator of soluble guanylyl cyclase, YC-1, caused a time- and dose-dependent loss of viability in clonal BRIN-BD11 beta-cells. This was accompanied by a rise in cGMP and was antagonised by Rp-8-pCPT-cGMPS, a selective inhibitor of protein kinase G (PKG). Reverse transcription polymerase chain reaction analysis confirmed that BRIN-BD11 cells (and human islets) express all three known isoforms of PKG (PKG-Ialpha, -Ibeta and II). Cell death induced by YC-1 was not sensitive to cell-permeable caspase inhibitors and was not accompanied by oligonucleosomal DNA fragmentation. The response was, however, inhibited by actinomycin D, suggesting that a transcription-dependent pathway of programmed cell death is involved in the actions of cGMP. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000188970100021
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被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50109
专题北京大学第三临床医学院_内分泌科
作者单位1.Peninsula Med Sch, Inst Biomed & Clin Sci, Endocrine Pharmacol Grp, Plymouth PL6 8BX, Devon, England
2.Peking Univ, Hosp 3, Dept Endocrinol, Beijing 100871, Peoples R China
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Kaminski, A,Gao, HW,Morgan, NG. Involvement of the cGMP signalling pathway in the regulation of viability in insulin-secreting BRIN-BD11 cells[J]. FEBS LETTERS,2004,559(1-3):118-124.
APA Kaminski, A,Gao, HW,&Morgan, NG.(2004).Involvement of the cGMP signalling pathway in the regulation of viability in insulin-secreting BRIN-BD11 cells.FEBS LETTERS,559(1-3),118-124.
MLA Kaminski, A,et al."Involvement of the cGMP signalling pathway in the regulation of viability in insulin-secreting BRIN-BD11 cells".FEBS LETTERS 559.1-3(2004):118-124.
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