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学科主题: 公共卫生
题名:
Epigenetic alterations in folate transport genes in placental tissue from fetuses with neural tube defects and in leukocytes from subjects with hyperhomocysteinemia
作者: Farkas, Sanja A.1; Bottiger, Anna K.1; Isaksson, Helena S.1,2; Finnell, Richard H.3; Ren, Aiguo4,5; Nilsson, Torbjorn K.1,2
关键词: FOLR1 ; PCFT ; RFC1 80G > ; A ; homocysteine ; tissue-specific DNA methylation ; GpG island ; NTD
刊名: EPIGENETICS
发表日期: 2013-03-01
DOI: 10.4161/epi.23988
卷: 8, 期:3, 页:303-316
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: CPG ISLAND SHORES ; DNA METHYLATION ; CARRIER GENE ; TRANSCRIPTIONAL REGULATION ; FOLIC-ACID ; EMBRYONIC-DEVELOPMENT ; CELLS ; HOMOCYSTEINE ; PROMOTER ; EXPRESSION
英文摘要:

The objectives of this study were to identify tissue-specific differentially methylated regions (T-DMR′s) in the folate transport genes in placental tissue compared with leukocytes, and from placental tissues obtained from normal infants or with neural tube defects (NTDs). Using pyrosequencing, we developed methylation assays for the CpG islands (CGIs) and the CGI shore regions of the folate receptor a (FOLR1), proton-coupled folate transporter (PCFT) and reduced folate carrier 1 (RFC1) genes. The T-DMRs differed in location for each gene and the difference in methylation ranged between 2 and 54%. A higher T-DMR methylated fraction was associated with a lower mRNA level of the FOLR1 and RFC1 genes. Methylation fractions differed according to RFC1 80G > A genotype in the NTD cases and in leukocytes from subjects with high total plasma homocysteine (tHcy). There were no differences in methylated fraction of folate transporter genes between NTD cases and controls. We suggest that T-DMRs participate in the regulation of expression of the FOLR1 and RFC1 genes, that the RFC1 80G > A polymorphism exerts a gene-nutrition interaction on DNA methylation in the RFC1 gene, and that this interaction appears to be most prominent in NTD-affected births and in subjects with high tHcy concentrations.

语种: 英语
所属项目编号: HD067244 ; ES021390 ; 2007CB5119001
项目资助者: Lions cancerfond ; Nyckelfonden ; Orebro lans landsting ; NIH ; State Key Development Program for Basic Research, People&prime ; s Republic of China
WOS记录号: WOS:000315923300008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50148
Appears in Collections:北京大学公共卫生学院_北京大学生育健康研究所_期刊论文

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作者单位: 1.Orebro Univ Hosp, Dept Lab Med, Orebro, Sweden
2.Univ Orebro, Sch Hlth & Med Sci, Orebro, Sweden
3.Univ Texas Austin, Dell Pediat Res Inst, Dept Nutr Sci, Austin, TX 78712 USA
4.Peking Univ, Hlth Sci Ctr, Inst Reprod & Child Hlth, Beijing 100871, Peoples R China
5.Peking Univ, Hlth Sci Ctr, Minist Hlth, Key Lab Reprod Hlth, Beijing 100871, Peoples R China

Recommended Citation:
Farkas, Sanja A.,Bottiger, Anna K.,Isaksson, Helena S.,et al. Epigenetic alterations in folate transport genes in placental tissue from fetuses with neural tube defects and in leukocytes from subjects with hyperhomocysteinemia[J]. EPIGENETICS,2013,8(3):303-316.
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