IR@PKUHSC  > 北京大学第二临床医学院  > 呼吸科
学科主题临床医学
Inhibition of allergic responsiveness in a murine asthma model via IFN-gamma transgene expression
Gao, ZC; Kang, Y; Xu, Y; Shang, Y; Gai, J; He, QY
关键词Gene Therapy Adenoviral Vector Interferon Type Ii Asthma
刊名CHINESE MEDICAL JOURNAL
2002-10-01
115期:10页:1470-1474
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]AIRWAY HYPERRESPONSIVENESS ; GENE-THERAPY ; INFLAMMATION ; CELLS
英文摘要

Objective To investigate adenoviral vector mediated exogenous gene expression in mouse lungs and the effect of mIFN-gamma transgene expression on allergen-induced pulmonary eosinophil infiltration in a murine asthmatic model.

Methods LacZ marker gene was transduced into CD-1 mouse airway epithelial cells by installation of a replication-deficient adenovirus with LacZ gene (AdCMVLacZ) 5 x 109 plaque forming unit (pfu) in the intratrachea or nostril. C57 mice were sensitized intraperitoneally and challenged by aerosol with ovalbumin (OVA) to produce an asthmatic model. AdCMVmIFNgamma 5 x 109 pfu was administered via nostril in asthmatic mice 48 h before OVA challenge. Sera, bronchial alveolar lavage (BAL) and lungs were recovered 48 h after OVA challenge.

Results After administration with AdCMVLacZ by intratracheal installation or nose-drop, the lungs revealed a high level of widespread LacZ transduction with X-gal staining, mainly along airways. IFN-gamma via adenoviral vector transduction could be overexpressed both in vitro and in vivo (1624.7 +/- 1321.5 pg/ml in BAL 96 h after AdCMVIFNgamma infection). In AdCMVIFNgamma treated asthmatic models, histological evaluation revealed marked suppression of eosinophil peribronchial and perivascular infiltration; the recoverable percentage of eosinophils in BAL was an average of 9.00% +/- 4.58%, which was a statistically significant decrease versus that of the positive control group (75.13% +/- 6.85%) ( P < 0.001). The total cell number in BAL ((145 +/- 55.6) X 10(3) cells/ml) in AdCMVmIFNgamma treated mice also was tremendously reduced compared to the positive control group ( (216.6 +/- 71.1) X 103 cells/ml).

Conclusions Adenoviral vector was able to overexpress exogenous gene in murine lungs. IFN-gamma overexpression via adenoviral vector in pulmonary epithelia in vivo can abrogate allergen-induced eosinophilic infiltration in lungs in an asthmatic model, which may suggest a new preventively therapeutic method for cytokine immunogenetic transfer in allergic asthma.

语种英语
WOS记录号WOS:000179018100008
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50158
Collection北京大学第二临床医学院_呼吸科
作者单位Peking Univ, Peoples Hosp, Dept Resp Med, Beijing 100044, Peoples R China
Recommended Citation
GB/T 7714
Gao, ZC,Kang, Y,Xu, Y,et al. Inhibition of allergic responsiveness in a murine asthma model via IFN-gamma transgene expression[J]. CHINESE MEDICAL JOURNAL,2002,115(10):1470-1474.
APA Gao, ZC,Kang, Y,Xu, Y,Shang, Y,Gai, J,&He, QY.(2002).Inhibition of allergic responsiveness in a murine asthma model via IFN-gamma transgene expression.CHINESE MEDICAL JOURNAL,115(10),1470-1474.
MLA Gao, ZC,et al."Inhibition of allergic responsiveness in a murine asthma model via IFN-gamma transgene expression".CHINESE MEDICAL JOURNAL 115.10(2002):1470-1474.
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