|Inhibition of allergic responsiveness in a murine asthma model via IFN-gamma transgene expression|
|Gao, ZC; Kang, Y; Xu, Y; Shang, Y; Gai, J; He, QY|
|关键词||Gene Therapy Adenoviral Vector Interferon Type Ii Asthma|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
|关键词[WOS]||AIRWAY HYPERRESPONSIVENESS ; GENE-THERAPY ; INFLAMMATION ; CELLS|
Objective To investigate adenoviral vector mediated exogenous gene expression in mouse lungs and the effect of mIFN-gamma transgene expression on allergen-induced pulmonary eosinophil infiltration in a murine asthmatic model.
Methods LacZ marker gene was transduced into CD-1 mouse airway epithelial cells by installation of a replication-deficient adenovirus with LacZ gene (AdCMVLacZ) 5 x 109 plaque forming unit (pfu) in the intratrachea or nostril. C57 mice were sensitized intraperitoneally and challenged by aerosol with ovalbumin (OVA) to produce an asthmatic model. AdCMVmIFNgamma 5 x 109 pfu was administered via nostril in asthmatic mice 48 h before OVA challenge. Sera, bronchial alveolar lavage (BAL) and lungs were recovered 48 h after OVA challenge.
Results After administration with AdCMVLacZ by intratracheal installation or nose-drop, the lungs revealed a high level of widespread LacZ transduction with X-gal staining, mainly along airways. IFN-gamma via adenoviral vector transduction could be overexpressed both in vitro and in vivo (1624.7 +/- 1321.5 pg/ml in BAL 96 h after AdCMVIFNgamma infection). In AdCMVIFNgamma treated asthmatic models, histological evaluation revealed marked suppression of eosinophil peribronchial and perivascular infiltration; the recoverable percentage of eosinophils in BAL was an average of 9.00% +/- 4.58%, which was a statistically significant decrease versus that of the positive control group (75.13% +/- 6.85%) ( P < 0.001). The total cell number in BAL ((145 +/- 55.6) X 10(3) cells/ml) in AdCMVmIFNgamma treated mice also was tremendously reduced compared to the positive control group ( (216.6 +/- 71.1) X 103 cells/ml).
Conclusions Adenoviral vector was able to overexpress exogenous gene in murine lungs. IFN-gamma overexpression via adenoviral vector in pulmonary epithelia in vivo can abrogate allergen-induced eosinophilic infiltration in lungs in an asthmatic model, which may suggest a new preventively therapeutic method for cytokine immunogenetic transfer in allergic asthma.
|作者单位||Peking Univ, Peoples Hosp, Dept Resp Med, Beijing 100044, Peoples R China|
|Gao, ZC,Kang, Y,Xu, Y,et al. Inhibition of allergic responsiveness in a murine asthma model via IFN-gamma transgene expression[J]. CHINESE MEDICAL JOURNAL,2002,115(10):1470-1474.|
|APA||Gao, ZC,Kang, Y,Xu, Y,Shang, Y,Gai, J,&He, QY.(2002).Inhibition of allergic responsiveness in a murine asthma model via IFN-gamma transgene expression.CHINESE MEDICAL JOURNAL,115(10),1470-1474.|
|MLA||Gao, ZC,et al."Inhibition of allergic responsiveness in a murine asthma model via IFN-gamma transgene expression".CHINESE MEDICAL JOURNAL 115.10(2002):1470-1474.|