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学科主题基础医学
Key role of microRNA-15a in the KLF4 suppressions of proliferation and angiogenesis in endothelial and vascular smooth muscle cells
Zhao, Liang1,3; Zheng, Xuemei1,3; Li, Aiqin1,3; Zhou, Tengfei1,3; Shen, Qiang1,3; Cui, Qinghua2,3; Qin, Xiaomei1,3
关键词Kruppel-like Factor 4 Mir-15a Proliferation Cell Cycle Cyclin D1
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2013-08-09
DOI10.1016/j.bbrc.2013.07.017
437期:4页:625-631
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]LAMINAR SHEAR-STRESS ; CYCLIN D1 ; EXPRESSION ; MOUSE ; MICE
英文摘要

While recent insights indicate that the transcription factor Kruppel-like factor 4 (KLF4) is indispensable for vascular homeostasis, its exact role in proliferation and angiogenesis and how it functions remain unresolved. Thus, the aim of the present study was to evaluate the role of KLF4 in the proliferations of endothelial and vascular smooth muscle cells, as well as the angiogenesis. The overexpression of KLF4 in endothelial cells significantly impaired tube formation. KLF4 inhibited the formation of a vascular network in implanted Matrigel plugs in nude mice. Importantly, we found that KLF4 significantly upregulated the miR-15a expression in endothelial cells and vascular smooth muscle cells, and conversely, KLF4 depletion reduced the amount of miR-15a. Furthermore, KLF4 blocked cell cycle progression and decreased cyclin D1 expression in endothelial cells and vascular smooth muscle cells through the induction of miR-15a. Intriguingly, the delivery of a miR-15a antagomir to nude mice resulted in marked attenuation of the anti-angiogenic effect of KLF4. Collectively, our present study provide the first evidence that miR-15a as a direct transcriptional target of KLF4 that mediates the anti-proliferative and anti-angiogenic actions of KLF4, which indicates that KLF4 upregulation of miR-15a may represent a therapeutic option to suppress proliferative vascular disorders. (C) 2013 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000323584400022
项目编号30700292 ; 30971160 ; 31271493
资助机构National Natural Science Foundation of China
引用统计
被引频次:20[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50165
专题北京大学基础医学院_心血管所
北京大学基础医学院
北京大学第三临床医学院_经营管理办公室
北京大学第三临床医学院_消化科
作者单位1.Peking Univ, Hlth Sci Ctr, Inst Cardiovasc Sci, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Biomed Informat, Beijing 100191, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Key Lab Mol Cardiovasc Sci, Minist Educ, Beijing 100191, Peoples R China
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Zhao, Liang,Zheng, Xuemei,Li, Aiqin,et al. Key role of microRNA-15a in the KLF4 suppressions of proliferation and angiogenesis in endothelial and vascular smooth muscle cells[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2013,437(4):625-631.
APA Zhao, Liang.,Zheng, Xuemei.,Li, Aiqin.,Zhou, Tengfei.,Shen, Qiang.,...&Qin, Xiaomei.(2013).Key role of microRNA-15a in the KLF4 suppressions of proliferation and angiogenesis in endothelial and vascular smooth muscle cells.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,437(4),625-631.
MLA Zhao, Liang,et al."Key role of microRNA-15a in the KLF4 suppressions of proliferation and angiogenesis in endothelial and vascular smooth muscle cells".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 437.4(2013):625-631.
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