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学科主题: 临床医学
题名:
Erroneous HbA(1c) measurements in the presence of beta-thalassemia and common Chinese hemoglobin variants
作者: Ji, Ling; Yu, Jing; Zhou, Yu; Xia, Yong; Xu, Anping; Li, Weining; Li, Lu
关键词: boronate affinity HPLC ; capillary electrophoresis ; HbA(1c) ; hemoglobin variants ; ion-exchange HPLC ; beta-thalassemia
刊名: CLINICAL CHEMISTRY AND LABORATORY MEDICINE
发表日期: 2015-08-01
DOI: 10.1515/cclm-2014-0598
卷: 53, 期:9, 页:1451-1458
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medical Laboratory Technology
研究领域[WOS]: Medical Laboratory Technology
关键词[WOS]: IFCC REFERENCE METHOD ; CAPILLARY-ELECTROPHORESIS ; A(1C) MEASUREMENT ; TRAITS ; ASSAY ; HB ; GLYCOHEMOGLOBIN ; DIAGNOSIS ; SEBIA ; HBA1C
英文摘要:

Background: HbA(1c) is a widely used biomarker for the management of diabetes mellitus and can be quantified from blood samples by using various methods, including ion-exchange high-performance liquid chromatography (HPLC), boronate affinity HPLC, and capillary electrophoresis (CE). Here, we evaluated the accuracy of four separation methods for determination of HbA(1c) values in Chinese patients with different hemoglobin disorders.

Methods: Blood samples from normal patients, patients with beta-thalassemia, patients exhibiting heterozygosity, and patients exhibiting homozygosity were analyzed using ion-exchange HPLC (Variant II Turbo, Bio-Rad and Adams A(1c) HA-8160, Arkray, run in diabetes mode), boronate affinity HPLC (Ultra(2), Trinity Biotech), and CE (Capillarys 2 Flex Piercing, Sebia).

Results: Samples from patients with beta-thalassemia produced significant positive biases on the Variant II Turbo system compared to the other three systems. For heterozygous beta A/beta E patients, a good agreement was observed between Capillarys 2 Flex Piercing and Ultra(2) systems, while a significant negative bias was observed between HA-8160 and Capillarys 2 Flex Piercing systems and between Variant II Turbo and Capillarys 2 Flex Piercing systems. For homozygous (beta E/beta E) patients, a clear context without HbA, all systems except the Capillarys 2 Flex Piercing system yielded random HbA(1c) results. Only the Capillarys 2 Flex Piercing system could detect all hemoglobin variants tested.

Conclusions: beta-Thalassemia can cause errors in HbA(1c) determination using the Variant II Turbo system. HbE heterozygosity or HbE homozygosity also complicated HbA(1c) measurements. The Capillarys 2 Flex Piercing system detected all Hb variants and HbA(1c) in patients with beta-thalassemia and could provide measurements with high accuracy.

语种: 英语
WOS记录号: WOS:000358583500028
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50178
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: Peking Univ, Shenzhen Hosp, Clin Med Lab, Shenzhen 518036, Guangdong, Peoples R China

Recommended Citation:
Ji, Ling,Yu, Jing,Zhou, Yu,et al. Erroneous HbA(1c) measurements in the presence of beta-thalassemia and common Chinese hemoglobin variants[J]. CLINICAL CHEMISTRY AND LABORATORY MEDICINE,2015,53(9):1451-1458.
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