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Erroneous HbA(1c) measurements in the presence of beta-thalassemia and common Chinese hemoglobin variants
Ji, Ling; Yu, Jing; Zhou, Yu; Xia, Yong; Xu, Anping; Li, Weining; Li, Lu
关键词Boronate Affinity Hplc Capillary Electrophoresis Hba(1c) Hemoglobin Variants Ion-exchange Hplc Beta-thalassemia
刊名CLINICAL CHEMISTRY AND LABORATORY MEDICINE
2015-08-01
DOI10.1515/cclm-2014-0598
53期:9页:1451-1458
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medical Laboratory Technology
研究领域[WOS]Medical Laboratory Technology
关键词[WOS]IFCC REFERENCE METHOD ; CAPILLARY-ELECTROPHORESIS ; A(1C) MEASUREMENT ; TRAITS ; ASSAY ; HB ; GLYCOHEMOGLOBIN ; DIAGNOSIS ; SEBIA ; HBA1C
英文摘要

Background: HbA(1c) is a widely used biomarker for the management of diabetes mellitus and can be quantified from blood samples by using various methods, including ion-exchange high-performance liquid chromatography (HPLC), boronate affinity HPLC, and capillary electrophoresis (CE). Here, we evaluated the accuracy of four separation methods for determination of HbA(1c) values in Chinese patients with different hemoglobin disorders.

Methods: Blood samples from normal patients, patients with beta-thalassemia, patients exhibiting heterozygosity, and patients exhibiting homozygosity were analyzed using ion-exchange HPLC (Variant II Turbo, Bio-Rad and Adams A(1c) HA-8160, Arkray, run in diabetes mode), boronate affinity HPLC (Ultra(2), Trinity Biotech), and CE (Capillarys 2 Flex Piercing, Sebia).

Results: Samples from patients with beta-thalassemia produced significant positive biases on the Variant II Turbo system compared to the other three systems. For heterozygous beta A/beta E patients, a good agreement was observed between Capillarys 2 Flex Piercing and Ultra(2) systems, while a significant negative bias was observed between HA-8160 and Capillarys 2 Flex Piercing systems and between Variant II Turbo and Capillarys 2 Flex Piercing systems. For homozygous (beta E/beta E) patients, a clear context without HbA, all systems except the Capillarys 2 Flex Piercing system yielded random HbA(1c) results. Only the Capillarys 2 Flex Piercing system could detect all hemoglobin variants tested.

Conclusions: beta-Thalassemia can cause errors in HbA(1c) determination using the Variant II Turbo system. HbE heterozygosity or HbE homozygosity also complicated HbA(1c) measurements. The Capillarys 2 Flex Piercing system detected all Hb variants and HbA(1c) in patients with beta-thalassemia and could provide measurements with high accuracy.

语种英语
WOS记录号WOS:000358583500028
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50178
专题北京大学深圳医院
北京大学第三临床医学院_感染疾病科
作者单位Peking Univ, Shenzhen Hosp, Clin Med Lab, Shenzhen 518036, Guangdong, Peoples R China
推荐引用方式
GB/T 7714
Ji, Ling,Yu, Jing,Zhou, Yu,et al. Erroneous HbA(1c) measurements in the presence of beta-thalassemia and common Chinese hemoglobin variants[J]. CLINICAL CHEMISTRY AND LABORATORY MEDICINE,2015,53(9):1451-1458.
APA Ji, Ling.,Yu, Jing.,Zhou, Yu.,Xia, Yong.,Xu, Anping.,...&Li, Lu.(2015).Erroneous HbA(1c) measurements in the presence of beta-thalassemia and common Chinese hemoglobin variants.CLINICAL CHEMISTRY AND LABORATORY MEDICINE,53(9),1451-1458.
MLA Ji, Ling,et al."Erroneous HbA(1c) measurements in the presence of beta-thalassemia and common Chinese hemoglobin variants".CLINICAL CHEMISTRY AND LABORATORY MEDICINE 53.9(2015):1451-1458.
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