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学科主题: 临床医学
题名:
Fetal Human Keratinocytes Produce Large Amounts of Antimicrobial Peptides: Involvement of Histone-Methylation Processes
作者: Gschwandtner, Maria1; Zhong, Shaomin1,2; Tschachler, Antonia1; Mlitz, Veronika1; Karner, Susanne1; Elbe-Buerger, Adelheid3; Mildner, Michael1
刊名: JOURNAL OF INVESTIGATIVE DERMATOLOGY
发表日期: 2014-08-01
DOI: 10.1038/jid.2014.165
卷: 134, 期:8, 页:2192-2201
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dermatology
研究领域[WOS]: Dermatology
关键词[WOS]: TOLL-LIKE RECEPTORS ; HUMAN SKIN ; AMNIOTIC-FLUID ; EPIDERMAL-KERATINOCYTES ; MAMMALIAN DEFENSINS ; PROTEIN PSORIASIN ; ESCHERICHIA-COLI ; VERNIX CASEOSA ; IMMUNITY ; ACTIVATION
英文摘要:

Antimicrobial peptides (AMPs), an important part of the innate immune system, are crucial for defense against invading microorganisms. Whereas AMPs have been extensively studied in adult skin, little is known about the impact of AMPs in the developing human skin. We therefore compared the expression and regulation of AMPs in fetal, neonatal, and adult keratinocytes (KCs) in vitro. The constitutive expression of human beta-defensin-2 (HBD-2), HBD-3, S100 protein family members, and cathelicidin was significantly higher in KCs from fetal skin than in KCs from postnatal skin. The capacity to further increase AMP production was comparable between prenatal and postnatal KCs. Analysis of skin equivalents (SEs) revealed a strong constitutive expression of S100 proteins in fetal but not in neonatal and adult SEs. The elevated AMP levels correlated with reduced H3K27me3 (tri-methyl-lysine 27 on histone H3) levels and increased expression of the histone demethylase JMJD3. Knockdown of JMJD3 in fetal KCs elevated H3K27me3 levels and significantly downregulated the expression of HBD-3, S100A7, S100A8, S100A9, and cathelicidin. Our data indicate a crucial contribution of histone modifications in the regulation of AMP expression in the skin during ontogeny. The elevated AMP expression in prenatal skin might represent an essential defense strategy of the unborn.

语种: 英语
所属项目编号: P19474-813 ; T545-B19
项目资助者: Austrian Science Fund
WOS记录号: WOS:000339126100020
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50196
Appears in Collections:北京大学第一临床医学院_皮肤性病科_期刊论文

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作者单位: 1.Med Univ Vienna, Dept Dermatol, Res Div Biol & Pathobiol Skin, A-1090 Vienna, Austria
2.Peking Univ, Dept Dermatol, Hosp 1, Beijing 100871, Peoples R China
3.Med Univ Vienna, Lab Cellular & Mol Immunobiol Skin, Div Immunol Allergy & Infect Dis, Dept Dermatol, A-1090 Vienna, Austria

Recommended Citation:
Gschwandtner, Maria,Zhong, Shaomin,Tschachler, Antonia,et al. Fetal Human Keratinocytes Produce Large Amounts of Antimicrobial Peptides: Involvement of Histone-Methylation Processes[J]. JOURNAL OF INVESTIGATIVE DERMATOLOGY,2014,134(8):2192-2201.
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