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Clinical Itraconazole-Resistant Strains of Aspergillus fumigatus, Isolated Serially from a Lung Aspergilloma Patient with Pulmonary Tuberculosis, can be Detected with Real-Time PCR Method
Xu, Hui1,2; Chen, Wei1,2; Li, Lijuan1,2; Wan, Zhe1,2; Li, Ruoyu1,2; Liu, Wei1,2
关键词Aspergillus Fumigatus Itraconazole Resistance Real-time Pcr Cyp51a Mutation
刊名MYCOPATHOLOGIA
2010-03-01
DOI10.1007/s11046-009-9249-x
169期:3页:193-199
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Mycology
研究领域[WOS]Mycology
关键词[WOS]INVASIVE ASPERGILLOSIS ; CROSS-RESISTANCE ; DRUG-RESISTANCE ; CYP51A ; SUSCEPTIBILITY ; EPIDEMIOLOGY ; DIAGNOSIS ; MECHANISMS ; GENE
英文摘要

The invasive aspergillosis, which is commonly caused by Aspergillus fumigatus (A. fumigatus), has increased in recent years. Traditional methods for finding out antifungal resistant strains would take more than 2 weeks, which cannot satisfy the needs of rapid detection. In this study, a real-time PCR method for detection of the serial itraconazole-resistant strains of A. fumigatus isolated from a lung aspergilloma patient was developed. The results showed that the TacMAN-MGB probes, which were covered the loci Gly54, Leu98, Gly138, and Met220 of the enzyme CYP51A coded by the gene cyp51A, as well as the 34-bp tandem repeated sequence in the promoter region (-288 and -322 from the start codon) of this gene, could detect the serial itraconazole-resistant strains of A. fumigatus in our study. Besides, this method takes just 6 h to complete the whole detection.

语种英语
WOS记录号WOS:000274656900006
项目编号30500027 ; 107002 ; 200802026 ; 2008ZX10004-002
资助机构National Natural Science Foundation of China ; Key Project of Chinese Ministry of Education ; Ministry of Health of the People&prime ; s Republic of China ; Ministry of Science and Technology of the People&prime ; s Republic of China
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50210
专题北京大学第一临床医学院_皮肤性病科
北京大学第三临床医学院_药剂科
作者单位1.Peking Univ, Hosp 1, Dept Dermatol, Beijing 100034, Peoples R China
2.Peking Univ, Res Ctr Med Mycol, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Xu, Hui,Chen, Wei,Li, Lijuan,et al. Clinical Itraconazole-Resistant Strains of Aspergillus fumigatus, Isolated Serially from a Lung Aspergilloma Patient with Pulmonary Tuberculosis, can be Detected with Real-Time PCR Method[J]. MYCOPATHOLOGIA,2010,169(3):193-199.
APA Xu, Hui,Chen, Wei,Li, Lijuan,Wan, Zhe,Li, Ruoyu,&Liu, Wei.(2010).Clinical Itraconazole-Resistant Strains of Aspergillus fumigatus, Isolated Serially from a Lung Aspergilloma Patient with Pulmonary Tuberculosis, can be Detected with Real-Time PCR Method.MYCOPATHOLOGIA,169(3),193-199.
MLA Xu, Hui,et al."Clinical Itraconazole-Resistant Strains of Aspergillus fumigatus, Isolated Serially from a Lung Aspergilloma Patient with Pulmonary Tuberculosis, can be Detected with Real-Time PCR Method".MYCOPATHOLOGIA 169.3(2010):193-199.
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