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学科主题: 临床医学
题名:
Penicillium marneffei SKN7, a Novel Gene, could Complement the Hypersensitivity of S. cerevisiae skn7 Disruptant Strain to Oxidative Stress
作者: Cao, Cunwei; Liu, Wei; Li, Ruoyu
关键词: Penicillium marneffei ; SKN7 ; Oxidative stress
刊名: MYCOPATHOLOGIA
发表日期: 2009-07-01
DOI: 10.1007/s11046-009-9192-x
卷: 168, 期:1, 页:23-30
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Mycology
研究领域[WOS]: Mycology
关键词[WOS]: PENICILLIUM-MARNEFFEI INFECTION ; SKN7 RESPONSE REGULATOR ; SACCHAROMYCES-CEREVISIAE ; CRYPTOCOCCUS-NEOFORMANS ; TRANSFORMATION ; EXPRESSION
英文摘要:

Penicillium marneffei is an intracellular fungal pathogen. Being resistant to the reactive oxygen species (ROS) produced by phagocytes is a key step to the surviving of P. marneffei within the phagocytes, as well as its invasion to the host. SKN7, a transcription factor, contributes to the oxidative stress response in yeasts. We cloned the SKN7 ortholog in P. marneffei and compared its transcription on both mycelial and yeast phase, identified its function by complement the S. cerevisiae skn7 disruptant strain. The result showed the full sequence of SKN7 gene was about 2.5 kb, open reading frame extended to 1,845 bp and encoded a putative protein of 614 amino acids. Comparative analysis of the nucleotide sequence of genomic DNA and cDNA confirmed the presence of four introns and two highly conserved HSF-DNA-bind and REC domain. The deduced amino acid sequence was homologous to SKN7 from other fungi. Further, P. marneffei cDNA can partly complement S. cerevisiae skn7 disruptant strain, which was not viable in the presence of 2.5 mM H(2)O(2). The expression level of SKN7 on the two phases, however, had no difference. These results indicated that P. marneffei SKN7 could response to oxidative stress and it was not a phase-specific gene.

语种: 英语
所属项目编号: 7072081
项目资助者: Specialized Research Fund for the Doctoral Program of Higher Education of China ; Beijing Municipal Natural Science Foundation
WOS记录号: WOS:000266926700003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50214
Appears in Collections:北京大学第一临床医学院_皮肤性病科_期刊论文

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作者单位: Peking Univ, Dept Dermatol, Res Ctr Med Mycol, Hosp 1, Beijing 100034, Peoples R China

Recommended Citation:
Cao, Cunwei,Liu, Wei,Li, Ruoyu. Penicillium marneffei SKN7, a Novel Gene, could Complement the Hypersensitivity of S. cerevisiae skn7 Disruptant Strain to Oxidative Stress[J]. MYCOPATHOLOGIA,2009,168(1):23-30.
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