|Screening of differentially expressed genes in the hypothalamus of a rat neuropathic pain model following sciatic nerve injury|
|Tang Yi3,4; Chu Guang-yue5; He Hong-xuan5; Yu Cui-ping1; An Jian-xiong1; Guo Xiang-yang2|
|关键词||Subtractiveneuropathic Pain Chronic Constriction Injury Differentially Expressed Gene Subtractive Suppressive Hybridization|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
|关键词[WOS]||CYTOCHROME-C-OXIDASE ; THERMAL HYPERALGESIA ; SPINAL-CORD ; PHOSPHORYLATION ; GLUTAMATE ; PATHWAY ; MICE|
Background Neuropathic pain is induced by injury or disease of the nervous system. Most studies have so far focused only on a few known molecules and signaling pathways among neurons. However, all signal transmissions involved in neuropathic pain appear to be an integral system at different molecular levels. This study was designed to screen the differentially expressed genes of the hypothalamus in chronic constriction injury (CCI) rats and analyze their functions in developing neuropathic pain.
Methods Ten adult female Sprague-Dawley rats ((200 +/- 10) g) were used in experimental group and sham group (n=5 in each group). Mechanical allodynia tests were performed to ensure that the CCI rat model was constructed successfully. Total hypothalamus RNAs were isolated from each group. Forward suppression subtractive hybridization (SSH) library of rat hypothalamus was constructed and up-regulated cDNA clones at neuropathic pain states were obtained via suppressed subtractive hybridization technique and the functions of these genes were analyzed bioinformatically.
Results Mechanical allodynia tests showed that the experimental rats had a significantly reduced mechanical allodynia threshold 3 to 13 days after CCI vs; sham surgery rats (P <0.01), indicating that the model was successful. Forward SSH library of the rat hypothalamus was constructed successfully and 26 over-expressed expression sequence tags (ESTs) were obtained from these up-regulated cDNA clones.
Conclusion Twenty-six up-regulated genes, involved in the regulation of cell cycle and apoptosis, signal transduction, and neuroprotection, may play key roles in decreasing mechanical withdraw thresholds in CCI rats, which implicates a multidimensional and integrated molecular mechanism at gene level in developing neuropathic pain with the supraspinal contributions. Chin Med J 2009;122(23):2893-2897
|资助机构||Tsinghua-Yue-Yuen Medical Sciences Fund|
|作者单位||1.Chinese Acad Sci, Inst Zool, Beijing 100101, Peoples R China|
2.Tsinghua Univ, Yuquan Hosp, Dept Anesthesia & Pain, Beijing 100044, Peoples R China
3.Peking Univ, Hosp 3, Dept Anesthesiol, Beijing 100191, Peoples R China
4.Chinese Acad Med Sci, Peking Union Med Coll Hosp, Dept Anesthesiol, Beijing 100730, Peoples R China
5.Peking Union Med Coll, Beijing 100730, Peoples R China
|Tang Yi,Chu Guang-yue,He Hong-xuan,et al. Screening of differentially expressed genes in the hypothalamus of a rat neuropathic pain model following sciatic nerve injury[J]. CHINESE MEDICAL JOURNAL,2009,122(23):2893-2897.|
|APA||Tang Yi,Chu Guang-yue,He Hong-xuan,Yu Cui-ping,An Jian-xiong,&Guo Xiang-yang.(2009).Screening of differentially expressed genes in the hypothalamus of a rat neuropathic pain model following sciatic nerve injury.CHINESE MEDICAL JOURNAL,122(23),2893-2897.|
|MLA||Tang Yi,et al."Screening of differentially expressed genes in the hypothalamus of a rat neuropathic pain model following sciatic nerve injury".CHINESE MEDICAL JOURNAL 122.23(2009):2893-2897.|