IR@PKUHSC  > 北京大学第一临床医学院
学科主题临床医学
Effects of Tissue Factor, PAR-2 and MMP-9 Expression on Human Breast Cancer Cell Line MCF-7 Invasion
Lin, Zeng-Mao; Zhao, Jian-Xin; Duan, Xue-Ning; Zhang, Lan-Bo; Ye, Jing-Ming; Xu, Ling; Liu, Yin-Hua
关键词Breast Cancer Tissue Factor Protease Activated Receptor-2 Matrix Metalloproteinase-9
刊名ASIAN PACIFIC JOURNAL OF CANCER PREVENTION
2014
DOI10.7314/APJCP.2014.15.2.643
15期:2页:643-646
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]FACTOR VIIA ; PKC-ALPHA ; MIGRATION ; SW620 ; PROLIFERATION ; INVOLVEMENT ; PROGRESSION ; ACTIVATION ; SURVIVAL ; PATHWAY
英文摘要

Objective: This study aimed to explore the expression of tissue factor (TF), protease activated receptor-2 (PAR-2), and matrix metalloproteinase-9 (MMP-9) in the MCF-7 breast cancer cell line and influence on invasiveness. Methods: Stable MCF-7 cells transfected with TF cDNA and with TF ShRNA were established. TF, PAR-2, and MMP-9 protein expression was analyzed using indirect immunofluorescence and invasiveness was evaluated using a cell invasion test. Effects of an exogenous PAR-2 agonist were also examined. Results: TF protein expression significantly differed between the TF cDNA and TF ShRNA groups. MMP-9 protein expression was significantly correlated with TF protein expression, but PAR-2 protein expression was unaffected. The PAR-2 agonist significantly enhanced MMP-9 expression and slightly increased TF and PAR-2 expression in the TF ShRNA group, but did not significantly affect protein expression in MCF-7 cells transfected with TF cDNA. TF and MMP-9 expression was positively correlated with the invasiveness of tumor cells. Conclusion: TF, PAR-2, and MMP-9 affect invasiveness of MCF-7 cells. TF may increase MMP-9 expression by activating PAR-2.

语种英语
WOS记录号WOS:000332517500018
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50243
专题北京大学第一临床医学院
作者单位Peking Univ, Hosp 1, Breast Dis Ctr, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Lin, Zeng-Mao,Zhao, Jian-Xin,Duan, Xue-Ning,et al. Effects of Tissue Factor, PAR-2 and MMP-9 Expression on Human Breast Cancer Cell Line MCF-7 Invasion[J]. ASIAN PACIFIC JOURNAL OF CANCER PREVENTION,2014,15(2):643-646.
APA Lin, Zeng-Mao.,Zhao, Jian-Xin.,Duan, Xue-Ning.,Zhang, Lan-Bo.,Ye, Jing-Ming.,...&Liu, Yin-Hua.(2014).Effects of Tissue Factor, PAR-2 and MMP-9 Expression on Human Breast Cancer Cell Line MCF-7 Invasion.ASIAN PACIFIC JOURNAL OF CANCER PREVENTION,15(2),643-646.
MLA Lin, Zeng-Mao,et al."Effects of Tissue Factor, PAR-2 and MMP-9 Expression on Human Breast Cancer Cell Line MCF-7 Invasion".ASIAN PACIFIC JOURNAL OF CANCER PREVENTION 15.2(2014):643-646.
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