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学科主题: 药学
题名:
Relationship between peptide structure and antimicrobial activity as studied by de novo designed peptides
作者: Liang, Dehai1,2; Sun, Jianbo1,2; Xia, Yuqiong3; Li, Dong4; Du, Quan4
关键词: Antimicrobial peptide ; Membrane leakage ; Fusion ; Aggregation
刊名: BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
发表日期: 2014-12-01
DOI: 10.1016/j.bbamem.2014.08.018
卷: 1838, 期:12, 页:2985-2993
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: MEMBRANE-FUSION ; PHOSPHATIDYLETHANOLAMINE LIPOSOMES ; ESCHERICHIA-COLI ; MAGAININ 2 ; MECHANISM ; VESICLES ; BACTERIA ; LENGTH ; PHASE ; DESTABILIZATION
英文摘要:

As fundamental components in innate immunity, antimicrobial peptides (AMPS) hold great potentials in the treatment of persistent infections involving slow-growing or dormant bacteria in which, selective inhibition of prokaryotic bacteria in the context of eukaryotic cells is not only an essential requirement, but also a critical challenge in the development of antimicrobial peptides. To identify the sequence and structural properties critical for antimicrobial activity, a series of peptides varying in sequence, length, hydrophobicity/charge ratio, and secondary structure, were designed and synthesized. Their antimicrobial activities were then tested using Escherichia coli and HEK293 cells, together with several index activities against model membrane, including liposome leakage, fusion, and aggregation. While no evident correlation between the antimicrobial activity and the property of the peptides was observed, common activities against model membrane were nevertheless identified for the active antimicrobial peptides: mediating efficient membrane leakage, negligible membrane fusion and liposome aggregation. Therefore, in addition to identifying one highly active antimicrobial peptide, our study further sheds light on the design principle for these molecules. (C) 2014 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 21074005 ; 21174007
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000343847200002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50245
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Coll Chem & Mol Engn, Minist Educ, Beijing 100871, Peoples R China
2.Peking Univ, Coll Chem & Mol Engn, Key Lab Polymer Chem & Phys, Minist Educ, Beijing 100871, Peoples R China
3.Xidian Univ, Sch Life Sci & Technol, Xian 710126, Shaanxi, Peoples R China
4.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100871, Peoples R China

Recommended Citation:
Liang, Dehai,Sun, Jianbo,Xia, Yuqiong,et al. Relationship between peptide structure and antimicrobial activity as studied by de novo designed peptides[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES,2014,1838(12):2985-2993.
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