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学科主题: 基础医学
题名:
Knockdown of hTERT Alters Biophysical Properties of K562 Cells Resulting in Decreased Migration Rate In Vitro
作者: Chen, Xiaopeng3; Zhang, Yingyu1,2; Xu, Xiaofeng4; Wang, Xianwei1,5; Wang, Xifu1; Yuan, Guihong6; Sun, Dagong6; Ka, Weibo6; He, Dongqi7; Wen, Zongyao1; Yao, Weijuan1
关键词: Telomerase ; Knockdown ; K562 ; Biophysics ; Migration
刊名: CELL BIOCHEMISTRY AND BIOPHYSICS
发表日期: 2011-12-01
DOI: 10.1007/s12013-011-9242-0
卷: 61, 期:3, 页:595-603
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
关键词[WOS]: MURINE ERYTHROLEUKEMIA-CELLS ; CHRONIC MYELOID-LEUKEMIA ; TELOMERASE ACTIVITY ; TRAIL GENE ; CANCER ; INHIBITION ; LINE ; VIVO ; DIFFERENTIATION ; OVEREXPRESSION
英文摘要:

It has been shown that 90% of tumors, including hematological malignant tumors and leukemia, have much higher levels of telomerase expression than normal cells. To investigate the effect of telomerase on leukemia cells, we transfected K562, a human erythroleukemia cell line with an antisense-hTERT (human telomerase reverse transcriptase) cDNA vector, and examined the biological and biophysical properties of the stably transfected cells (referred to as KAT). Un-transfected cells (K562) and cells transfected with the empty vector (referred to as KC) were used as controls. Cell growth curve and (3)H-TdR test showed that the growth rate and DNA synthesis of KAT decreased compared with those of K562 and KC cells. Apoptosis and cell cycle distribution in KAT cells under normal culture condition were similar to those of K562 and KC cells, but changed after serum deprivation. KAT cells had significantly different biophysical characteristics from K562 and KC in terms of cell electrophoresis, membrane fluidity, membrane fluidity, and viscoelasticity. Furthermore, the transendothelial migration rate of KAT was much lower than those of K562 and KC cells. Confocal microscopy showed that KAT cells had higher F-actin content, suggesting the reorganization of cytoskeleton. Flow cytometry analysis revealed a lowered intracellular calcium concentration and CD71 expression, explaining the high F-actin content in KAT cells. In conclusion, we found that the knockdown of hTERT in K562 cells changed their cytoskeleton and biophysical features, and reduced the cell migration.

语种: 英语
所属项目编号: 10572007 ; 30770532
项目资助者: National Natural Science Foundations of China
WOS记录号: WOS:000297163300013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50250
Appears in Collections:基础医学院_北京大学血液流变中心_期刊论文

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作者单位: 1.Peking Univ, Hemorheol Ctr, Hlth Sci Ctr, Beijing 100191, Peoples R China
2.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China
3.Chengde Med Coll, Chengde 067000, Peoples R China
4.Capital Med Univ, Inst Clin Lab, Beijing TongRen Hosp, Beijing 100730, Peoples R China
5.Peking Univ, Dept Cardiol, Shougang Hosp, Beijing 100041, Peoples R China
6.Peking Univ, Dept Med Phys, Hlth Sci Ctr, Beijing 100191, Peoples R China
7.Peking Univ, Dept Biomath & Biostat, Hlth Sci Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Chen, Xiaopeng,Zhang, Yingyu,Xu, Xiaofeng,et al. Knockdown of hTERT Alters Biophysical Properties of K562 Cells Resulting in Decreased Migration Rate In Vitro[J]. CELL BIOCHEMISTRY AND BIOPHYSICS,2011,61(3):595-603.
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