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学科主题临床医学
Overexpression of p42.3 promotes cell growth and tumorigenicity in hepatocellular carcinoma
Sun, Wei1; Dong, Wei-Wei2; Mao, Lin-Lin3; Li, Wen-Mei1; Cui, Jian-Tao1; Xing, Rui1; Lu, You-Yong1
关键词P42.3 Hepatocellular Carcinoma Hepg2 Overexpression Tumorigenicity
刊名WORLD JOURNAL OF GASTROENTEROLOGY
2013-05-21
DOI10.3748/wjg.v19.i19.2913
19期:19页:2913-2920
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]MITOTIC SPINDLE CHECKPOINT ; CDC25 PHOSPHATASES ; GASTRIC-CANCER ; DIFFERENTIAL EXPRESSION ; CYCLE CONTROL ; MAD2 ; MITOSIS ; PROTEIN ; IDENTIFICATION ; EPIDEMIOLOGY
英文摘要

AIM: To investigate the association of p42.3 expression with clinicopathological characteristics and the biological function of p42.3 in human hepatocellular carcinoma (HCC).

METHODS: We used reverse transcription-polymerase chain reaction (RT-PCR), quantitative real-time RT-PCR and western blotting to detect p42.3 mRNA and protein expression in hepatic cell lines. We examined primary HCC samples and matched adjacent normal tissue by immunohistochemistry to investigate the correlation between p42.3 expression and clinicopathological features. HepG2 cells were transfected with a pIRES2-EGFP-p42.3 expression vector to examine the function of the p42.3 gene. Transfected cells were analyzed for their viability and malignant transformation abilities by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, colony formation assay, and tumorigenicity assay in nude mice.

RESULTS: p42.3 is differentially expressed in primary HCC tumors and cell lines. Approximately 69.6% (96/138) of cells were p42.3-positive in hepatic tumor tissues, while 30.7% (35/114) were p42.3-positive in tumor-adjacent normal tissues. Clinicopathological characteristics of the HCC specimens revealed a significant correlation between p42.3 expression and tumor differentiation (P = 0.031). However, p42.3 positivity was not related to tumor tumor-node-metastasis classification, hepatitis B virus status, or hepatoma type. Regarding p42.3 overexpression in stably transfected HepG2 cells, we discovered significant enhancement of cancer cell growth and colony formation in vitro, and significantly enhanced tumorigenicity in nude mice. Western blot analysis of cell cycle proteins revealed that enhanced p42.3 levels promote upregulation of proliferating cell nuclear antigen, cyclin B1 and mitotic arrest deficient 2.

CONCLUSION: p42.3 promotes tumorigenicity and tumor growth in HCC and may be a potential target for future clinical cancer therapeutics. (C) 2013 Baishideng. All rights reserved.

语种英语
WOS记录号WOS:000319334100007
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50261
专题北京大学临床肿瘤学院_分子肿瘤学研究室
作者单位1.Peking Univ, Mol Oncol Lab, Key Lab Carcinogenesis & Translat Res, Minist Educ,Canc Hosp Inst, Beijing 100142, Peoples R China
2.Gen Hosp Chinese Peoples Liberat Army, Dept Med Oncol, Beijing 100853, Peoples R China
3.Xuzhou Med Coll, Jiangsu Key Lab Biol Canc Therapy, Xuzhou 221002, Jiangsu, Peoples R China
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GB/T 7714
Sun, Wei,Dong, Wei-Wei,Mao, Lin-Lin,et al. Overexpression of p42.3 promotes cell growth and tumorigenicity in hepatocellular carcinoma[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2013,19(19):2913-2920.
APA Sun, Wei.,Dong, Wei-Wei.,Mao, Lin-Lin.,Li, Wen-Mei.,Cui, Jian-Tao.,...&Lu, You-Yong.(2013).Overexpression of p42.3 promotes cell growth and tumorigenicity in hepatocellular carcinoma.WORLD JOURNAL OF GASTROENTEROLOGY,19(19),2913-2920.
MLA Sun, Wei,et al."Overexpression of p42.3 promotes cell growth and tumorigenicity in hepatocellular carcinoma".WORLD JOURNAL OF GASTROENTEROLOGY 19.19(2013):2913-2920.
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