IR@PKUHSC  > 北京大学基础医学院
学科主题基础医学
Intermedin(1-53) attenuates vascular smooth muscle cell calcification by inhibiting endoplasmic reticulum stress via cyclic adenosine monophosphate/protein kinase A pathway
Zhu, Yi1,2; Chang, Jin-Rui1,2; Duan, Xiao-Hui2; Zhang, Bao-Hong3; Teng, Xu2; Zhou, Ye-Bo2; Liu, Yue4; Yu, Yan-Rong5; Tang, Chao-Shu1,2; Qi, Yong-Fen2,5
关键词Intermedin(1-53) Vascular Calcification Endoplasmic Reticulum Stress Apoptosis
刊名EXPERIMENTAL BIOLOGY AND MEDICINE
2013-10-01
DOI10.1177/1535370213502619
238期:10页:1136-1146
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, Research & Experimental
研究领域[WOS]Research & Experimental Medicine
关键词[WOS]SIGNALING PATHWAY ; ADRENOMEDULLIN ; APOPTOSIS ; PEPTIDE ; PROTEIN ; INJURY ; MECHANISMS ; RECEPTOR ; RATS
英文摘要

We previously reported that endoplasmic reticulum (ER) stress-mediated apoptosis participated in vascular calcification. Importantly, a novel paracrine/autocrine peptide intermedin(1-53) (IMD1-53) in the vasculature inhibited vascular calcification in rats. But the mechanisms needed to be fully elucidated. Vascular smooth muscle cells (VSMCs) calcification was induced by CaCl2 and beta-glycerophosphate. Tunicamycin (Tm) or dithiothreitol (DTT) was used to induce ER stress. We found that IMD1-53 (10(-7) mol/L) treatment significantly alleviated the protein expression of ER stress hallmarks activating transcription factor 4 (ATF4), ATF6, glucose-regulated protein 78 (GRP78) and GRP94 induced by Tm or DTT. ER stress occurred in early and late calcification of VSMCs but was inhibited by IMD1-53. These inhibitory effects of IMD1-53 were abolished by treatment with the protein kinase A (PKA) inhibitor H89. Pretreatment with IMD1-53 decreased the number of apoptotic VSMCs and downregulated protein expression of cleaved caspase 12 and C/EBP homologous protein (CHOP) in calcified VSMCs. Concurrently, IMD1-53 restored the loss of VSMC lineage markers and ameliorated calcium deposition and alkaline phosphatase activity in calcified VSMCs as well. The observation was further verified by Alizarin Red S staining, which showed that IMD1-53 reduced positive red nodules among calcified VSMCs. In conclusion, IMD1-53 attenuated VSMC calcification by inhibiting ER stress through cAMP/PKA signalling.

语种英语
WOS记录号WOS:000324914200006
项目编号81270407 ; 81170082 ; 81100229 ; 20110001110012
资助机构National Natural Science Foundation of China ; Ministry of Education Foundation for Doctoral Tutor ; Leading Academic Discipline Project of Beijing Education Bureau
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50274
专题北京大学基础医学院
作者单位1.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Sch Basic Med Sci, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Key Lab Mol Cardiovasc Sci, Minist Educ, Beijing 100191, Peoples R China
3.Capital Inst Pediat, Beijing 100020, Peoples R China
4.Harbin Med Univ, Dept Cardiol, Affiliated Hosp 1, Harbin 150001, Peoples R China
5.Peking Univ, Lab Cardiovasc Bioact Mol, Sch Basic Med Sci, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Zhu, Yi,Chang, Jin-Rui,Duan, Xiao-Hui,et al. Intermedin(1-53) attenuates vascular smooth muscle cell calcification by inhibiting endoplasmic reticulum stress via cyclic adenosine monophosphate/protein kinase A pathway[J]. EXPERIMENTAL BIOLOGY AND MEDICINE,2013,238(10):1136-1146.
APA Zhu, Yi.,Chang, Jin-Rui.,Duan, Xiao-Hui.,Zhang, Bao-Hong.,Teng, Xu.,...&Qi, Yong-Fen.(2013).Intermedin(1-53) attenuates vascular smooth muscle cell calcification by inhibiting endoplasmic reticulum stress via cyclic adenosine monophosphate/protein kinase A pathway.EXPERIMENTAL BIOLOGY AND MEDICINE,238(10),1136-1146.
MLA Zhu, Yi,et al."Intermedin(1-53) attenuates vascular smooth muscle cell calcification by inhibiting endoplasmic reticulum stress via cyclic adenosine monophosphate/protein kinase A pathway".EXPERIMENTAL BIOLOGY AND MEDICINE 238.10(2013):1136-1146.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Intermedin(1-53) att(950KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhu, Yi]的文章
[Chang, Jin-Rui]的文章
[Duan, Xiao-Hui]的文章
百度学术
百度学术中相似的文章
[Zhu, Yi]的文章
[Chang, Jin-Rui]的文章
[Duan, Xiao-Hui]的文章
必应学术
必应学术中相似的文章
[Zhu, Yi]的文章
[Chang, Jin-Rui]的文章
[Duan, Xiao-Hui]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Intermedin(1-53) attenuates vascular smooth muscle cell calcification by inhibiting endoplasmic reticulum stress via cyclic adenosine monophosphate_protein kinase A pathway.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。